Characterization of candidate genes related to the high aluminum tolerant mechanisms in a wild plant, Andropogon virginicus
| Project/Area Number |
23580092
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Okayama University |
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Principal Investigator |
EZAKI Bunichi 岡山大学, 資源植物科学研究所, 准教授 (90243500)
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| Co-Investigator(Kenkyū-buntansha) |
SONODA Syouji 岡山大学, 資源植物科学研究所, 准教授 (00325127)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 植物栄養代謝 / イネ科有用野生植物 / メリケンカルカヤ / AvSAMS1遺伝子 / AvABCG1 transporter 遺伝子 / アルミニウムストレス / ストレス耐性機構 / エピジェネティックス / ストレス応答機構 / SAMS遺伝子 / ABC transporter 遺伝子 / epigenetics / 遺伝子発現調節 / イネ科野生植物 / 金属ストレス / 酸化ストレス / 耐性遺伝子 / ストレス誘導性遺伝子 / 遺伝子発現 / 形質転換植物 |
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| Research Abstract |
To clarify high Al tolerance mechanism in a poaceae wild plant, Andropogon virginicus L., its two candidate genes, S-adenosyl methionine synthase (AvSAMS1) and ABC transporter (AvABCG1), were characterized. The constructed two Arabidopsis transgenic lines expressing these two genes showed multiple tolerance phenotypes for Al, Cu, diamide or Zn. Microarray analysis and other experiments suggested that Al stress causes a dynamic change in DNA methylation status and that the AvSAMS1 gene is related to an epigenetic control of gene-expression under Al stress. It was also suggested that the AvABCG1transporter functions for an accumulation of toxic Al and Pb in root and/or leaf, rather than a transportation of these metals from root to shoot.
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Report
(4 results)
Research Products
(33 results)
