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Comparison of functions on intestinal microbes between strains of Bifidobacterium and Ruminococcus.

Research Project

Project/Area Number 23580097
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionHokkaido University

Principal Investigator

ASANO Kozo  北海道大学, 大学院・農学研究院, 教授 (50312393)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
KeywordsHigh Fat / Health Effect / Intestinal Bacteria / Ruminococcus / Bifidobacterium / DFAIII / FOS / Yacon / Fruct Oligosaccharide (FOS) / Difructose Anhydride III (DFAIII) / 腸内細菌
Research Abstract

In-digestible saccharides are important to control microbiota in colon. Fructo-oligosaccharide (FOS) is a typical in-digestible oligosaccharide, and it increase cells of Bifidobacterium species. We made process to make different in-digestible oligosaccharide which is DFAIII (di-fructose anhydride III) with using microbial enzyme effectively. DFAIII enhanced growth of Ruminococcus productus AHU1760 which was isolated from human feces, but not growth of Bifidobacterium strains because Bifidobacterium strains can’t degrade DFAIII. In this study we compared health effect of DFAIII + Ruminococcus productus AHU1760 vs. FOS + Bifidobacterium breve JCM1192T. Both combination were symbiotic. Yacon which is FOS rich natural vegetable were used as comparison with reagent FOS. Rat used were SD male 5 weeks with normal feed and high fat feed were used. Rats were kept for 30 days and analyzed pH, organic acid compositions, microbiota in cecum with using HPLC, DNA PCR-DGGE.
Both symbiotic showed clear pH decrease in cecal contents and feces which indicated abundant intestinal fermentation by abundant growth of microbes. Differences were observed in high fat conditions. Symbiotic by DFAIII and Ruminococcus productus AHU1760 continued abundant intestinal fermentations and kept intestinal pH in lower condition but not symbiotic by FOS and Bifidobacterium breve JCM1192T. Those results were supported DNA PCR-DGGE analysis. Ruminococcus productus AHU1760 showed better health effect than Bifidobacterium breve JCM1192T. Yacon died gave faster change of intestinal microbiota and pH which shoeing better health effect than reagent FOS.

Report

(3 results)
  • 2013 Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (18 results)

All 2013 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (17 results)

  • [Journal Article] Comparison of yacon (Smallanthus sonchifolius) tuber to commercialized fructo-oligosaccharides (FOS) in terms of physiology, fermentation products, and intestinal microbial communities in rats2013

    • Author(s)
      Utami, N. W. A., Sone, T., Tanaka, M., Nakatsu, C. H., Saito, A. and Asano, K.
    • Journal Title

      Biosci. Microbiota, Food and Health

      Volume: 32 Pages: 167-178

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Oligosaccharide (DFAIII) production from dahlia tuber with actinomycete Nonomuraea sp2013

    • Author(s)
      Nanami Takano1, Sri Pudjirahatri, Midori Ohtani1, Keisuke Sugimoto, Teruo Sone1, Michiko Tanaka1, Kozo Asano
    • Organizer
      Yang scientists seminar
    • Place of Presentation
      Osaka
    • Year and Date
      2013-08-07
    • Related Report
      2013 Final Research Report
  • [Presentation] オリゴ糖DFAIIIとRuminococcus productus AHU1760 のシンバイオティクス効果について2013

    • Author(s)
      藤井早苗、ヴェロニカ・バウア、川並かおる、曽根輝夫、田中みち子、浅野行蔵
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      仙台市
    • Year and Date
      2013-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] オリゴ糖DFA IIIとRuminococcus productus AHU1760のシンバイオティクス効果について2013

    • Author(s)
      藤井早苗, ヴェロニカ・バウア, 川並かおる, 曽根輝雄, 田中みち子, 浅野行蔵
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      仙台市
    • Year and Date
      2013-03-27
    • Related Report
      2012 Research-status Report
  • [Presentation] 異なったオリゴ糖摂取による腸内環境の相違2012

    • Author(s)
      バルア・ヴェロニカ・ラファエル、藤井早苗、川並かおる、曾根輝雄、田中みち子、浅野行蔵
    • Organizer
      日本栄養改善学会北海道支部会
    • Place of Presentation
      札幌市
    • Year and Date
      2012-12-05
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Feeding Yacon Tubercontaining Oligofructose Increases Intestinal Fermentation and Alters Microbial Community Structure in Rats2012

    • Author(s)
      Ni Wayan Arya Utami, Michiko Tanaka, Teruo Sone, Cindy Nakatsu, Kozo Asano
    • Organizer
      The 4th International Conference for Biosciences and Biotechnology (ICBB)
    • Place of Presentation
      インドネア国バリ島
    • Year and Date
      2012-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Feeding Yacon Tuber-containing Oligofructose Increases Intestinal Fermentation and Alters Microbial Community Structure in Rats2012

    • Author(s)
      Ni Wayan Arya Utami, Michiko Tanaka, Teruo Sone, Cindy Nakatsu, Kozo Asano
    • Organizer
      The 4th International Conference for Biosciences and Biotechnology (ICBB)
    • Place of Presentation
      インドネア国バリ島
    • Year and Date
      2012-09-20
    • Related Report
      2012 Research-status Report
  • [Presentation] DFAIIIとRuminococcus productus AHU1760 の投与による腸内環境への影響2012

    • Author(s)
      藤井早苗、田中みち子、曾根輝雄、中津シンディ、浅野行蔵
    • Organizer
      食品科学工学会大会第59回大会
    • Place of Presentation
      札幌市
    • Year and Date
      2012-08-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Effects of yacon tuber-containing oligofructose on intestinal short chain fatty acids and microbial ecology in rats2012

    • Author(s)
      Utami Ni Wayan Arya, Tanaka Michiko, Sone Teruo, Nakatsu Cindy H, Asano Kozo
    • Organizer
      日本食品科学工学会第59回大会
    • Place of Presentation
      札幌市
    • Year and Date
      2012-08-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Effects of yacon tuber-containing oligofructose on intestinal short chain fatty acids and microbial ecology in rats.2012

    • Author(s)
      Utami Ni Wayan Arya, Tanaka Michiko, Sone Teruo, Nakatsu Cindy H, Asano Kozo
    • Organizer
      日本食品科学工学会第59回大会
    • Place of Presentation
      札幌市
    • Year and Date
      2012-08-30
    • Related Report
      2012 Research-status Report
  • [Presentation] DFAIIIとRuminococcus productus AHU1760の投与による腸内環境への影響2012

    • Author(s)
      藤井早苗, 田中みち子, 曾根輝雄, 中津シンデイ, 浅野行蔵
    • Organizer
      食品科学工学会大会第59回大会
    • Place of Presentation
      札幌市
    • Year and Date
      2012-08-30
    • Related Report
      2012 Research-status Report
  • [Presentation] Feeding yacon tuber increases intestinal fermentation and alters microbial community structure in rats2012

    • Author(s)
      Utami, N.A., Tanaka, M., Sone, T., Nakatsu, C.H., Asano, K
    • Organizer
      14th International Society of Microbial Ecology
    • Place of Presentation
      デンマーク国コペンハーゲン市
    • Year and Date
      2012-08-22
    • Related Report
      2013 Final Research Report
  • [Presentation] Feeding yacon tuber increases intestinal fermentation and alters microbial community structure in rats2012

    • Author(s)
      Utami, NA, Tanaka, M, Sone, T, Nakatsu, C. H., Asano, K
    • Organizer
      14th International Society of Microbial Ecology.
    • Place of Presentation
      デンマーク国コペンハーゲン市
    • Year and Date
      2012-08-22
    • Related Report
      2012 Research-status Report
  • [Presentation] Yacon increased intestinal fermentation and changed microbial communities in rats2012

    • Author(s)
      Utami NA, Tanaka, M., Sone, T., Nakatsu, C.H., Asano, K.
    • Organizer
      日本腸内細菌学会第16回大会
    • Place of Presentation
      神戸市
    • Year and Date
      2012-06-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Yacon increased intestinal fermentation and changed microbial communities in rats.2012

    • Author(s)
      Utami NA, Tanaka, M., Sone, T, Nakatsu, C. H., Asano, K.
    • Organizer
      日本腸内細菌学会第16回大会
    • Place of Presentation
      神戸市
    • Year and Date
      2012-06-13
    • Related Report
      2012 Research-status Report
  • [Presentation] DFAIII とRuminococcus productus AHU1760 の投与による腸内環境への影響

    • Author(s)
      藤井早苗,田中みち子,曾根輝雄,中津シンディ,浅野行蔵
    • Organizer
      (社)日本食品科学工学会 北海道支部大会
    • Place of Presentation
      藤女子大学(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] Feeding rats Yacon powder increases intestinal fermentation and alters microbial community profiles

    • Author(s)
      Ni Wayan Arya Utami,田中みち子,曾根輝雄,中津シンディ,浅野行蔵
    • Organizer
      (社)日本食品科学工学会 北海道支部大会
    • Place of Presentation
      藤女子大学(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] Feeding rats yacon powder increases intestinal fermentation and alters microbial community profiles

    • Author(s)
      Arya UTAMI Ni Wayan, Michiko Tanaka, Teruo Sone, Cindy Nakatsu, Kozo Asano
    • Organizer
      (社)日本農芸化学会 2012年大会
    • Place of Presentation
      京都女子大(京都府)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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