Construction of gene expression system using virulent phages.

• Project/Area Number: 23580100
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Tohoku University
• Principal Investigator: KANEKO JUN 東北大学, (連合)農学研究科(研究院), 准教授 (30221188)
• Co-Investigator(Renkei-kenkyūsha): KIMURA Keitaro (独)農業・食品産業技術総合研究機構, 食品総合研究所, 主任研究員 (20353980)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 納豆菌 virulent phages / 感染補助因子 / 遺伝子発現 / 宿主認識 / 納豆菌ファージ / 形質転換系

Research Abstract

Some Bacillus subtilis natto phages carry gamma-poly glutamate hydrolase gene on their genome, and this gene is strongly expressed during phage lytic cycle. To apply this system for the foreign gene expression system, we determined complete genome sequence of phage phiNIT1 and analyzed the expression system of gamma-PGA hydrolase gene.
As a result, we found that not only phage encoded elements but also both of cis-elements and trans-elements in the bacterium were required for the gene expression. In addition, we identified phage proteins involving in specific recognition of host cell surface.

Research Products

• [Journal Article] AxyR, an AraC Family Transcriptional Activator of the Xylanase 3 Gene, Requires Xylo-Oligosaccharides as a Cofactor for DNA Binding in <i>Paenibacillus</i> sp. Strain W-61 (2012)
  • Author(s): M. Fukuda, S. Yoshida, H. Itoh, Y. Kato, S. Watanabe, Y. Itoh, Y. Kamio, J. Kaneko
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 5 Pages: 1051-1054
  • DOI: 10.1271/bbb.120165
  • NAID: 10030753385
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] AxyR, an AraA family transcriptional activator of the xylanase 3 gene, requires xylo-oligosaccharides as a cofactor for DNA binding in Paenibacillus sp. Strain W-61 (2012)
  • Author(s): Fukuda, M., Yoshida, S., Itoh, H., Kato, Y., Watanabe, S., Itoh, Y., Kami, Y., Kaneko, J.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 76 Pages: 1051-1054
  • Peer Reviewed
• [Journal Article] Crystal structure of the octameric pore of staphylococcal γ-hemolysin reveals the β-barrel pore formation mechanism by two components (2011)
  • Author(s): Keitaro Yamashita, Yuka Kawai, Yoshikazu Tanaka, Nagisa Hirano, Jun Kaneko, Noriko Tomita, Makoto Ohta, Yoshiyuki Kamio, Min Yao, Isao Tanaka
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 108 Issue: 42 Pages: 17314-17319
  • DOI: 10.1073/pnas.1110402108
  • Peer Reviewed
• [Journal Article] Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis (2011)
  • Author(s): T.-H. Do, Y. Suzuki, N. Abe, J. Kaneko, Y. Itoh and K. Kimura
  • Journal Title: Appl. Environ. Microbiol Volume: 77 Issue: 23 Pages: 8249-8258
  • DOI: 10.1128/aem.05827-11
  • Peer Reviewed
• [Presentation] ファージ尾部状バクテリオシンを利用したファージ宿主認識の研究 (2014)
  • Author(s): 樋口大祐、鈴木敦斗、木村啓太郎、阿部直樹、金子淳
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 川崎市明治大学農学部
• [Presentation] 納豆菌ファージにおける菌体外ポリマー分解酵素遺伝子の存在とその転写制御機構の解析 (2012)
  • Author(s): 鈴木敦斗、尾崎達郎、木村啓太郎、金子淳
  • Organizer: 第4回ファージ研究会
  • Place of Presentation: 前橋市群馬大学刀城会館
• [Presentation] 納豆菌ファージにおける菌体外ポリマー分解酵素遺伝子の 存在とその転写制御機構の解析 (2012)
  • Author(s): 鈴木敦斗、尾崎達郎、木村啓太郎、金子淳
  • Organizer: 第４回ファージ研究会
  • Place of Presentation: 前橋市 群馬大学 刀城会館
• [Presentation] ファージ尾部状バクテリオシンを利用したファージ宿主認識の研究
  • Author(s): 樋口大祐、鈴木敦斗、木村啓太郎、阿部 直樹、金子 淳
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学生田キャンパス