| Project/Area Number |
23580101
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Nihon University (2013)
Tohoku University (2011-2012) |
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Principal Investigator |
KAWAI Yasushi 日本大学, 生物資源科学部, 准教授 (00261496)
|
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Tadao 東北大学, 農学研究科, 教授 (00118358)
西村 順子 東北大学, (連合)農学研究科(研究院), その他 (10241556)
|
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIMURA Junko 東北大学, 農学研究科, 技術専門職員 (10241556)
|
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
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| Keywords | バクテリオシン / 抗菌ペプチド / 環状構造 / 乳酸菌 / ガセリ菌 |
|---|
| Research Abstract |
A biosynthesis genes for gassericin A, which was the cyclic antibacterial peptide (bacteriocin) linked at N- and C-termini consisting of 58 amino acid residue, were identified, and gene expression analysis clarified that the leader sequence was important to production of gassericin A not the cyclization. In addition, it was suggested gassericin A was linked at N- and C-terminal amino acids by an ABC-transporter and then secreted to outside the producer cells after cleavage of the leader peptide by a putative novel proteinase.
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