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Arsenic release from paddy soil by a dissimilatory arsenate-reducing bacterium Geobacter sp. OR-1

Research Project

Project/Area Number 23580103
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionChiba University

Principal Investigator

AMACHI Seigo  千葉大学, 園芸学研究科, 准教授 (80323393)

Co-Investigator(Renkei-kenkyūsha) YAMAGUCHI Noriko  (独)農業環境技術研究所, 土壌環境研究領域, 主任研究員 (80345090)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords環境微生物 / ヒ素 / 水田土壌 / 溶出 / Geobacter / ヒ酸還元 / ArrA / XANES / 鉄還元 / XAFS / 水田 / 汚染 / 微生物 / Anaeromyxobacter / ヒ素汚染
Research Abstract

Previously, we have succeeded to isolate a novel arsenate-reducing bacterium Geobacter sp. OR-1 from Japanese paddy soil. In this study, we isolated a new arsenate-reducing bacterium Anaeromyxobacter sp. PSR-1 from arsenic-polluted soil. When these bacterial strains were inoculated into sterile paddy soil, arsenic release was reproduced. XANES analysis revealed that these strains were able to reduce arsenate adsorbed on soil solid phase. In addition, the strains could release arsenic adsorbed on ferrihydrite. We also determined draft genome sequences of these strains. Preliminary draft genome analysis of strain OR-1 identified two arsenic islands containing multiple arsA and arsD genes, and possible dissimilatory arsenate-reductase genes (arrAB) were also found.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (28 results)

All 2014 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (17 results) Book (2 results)

  • [Journal Article] Microbiology of inorganic arsenic : From metabolism to bioremediation2014

    • Author(s)
      S. Yamamura and S. Amachi
    • Journal Title

      J. Biosci. Bioeng

      Volume: (in press) Issue: 1 Pages: 1-9

    • DOI

      10.1016/j.jbiosc.2013.12.011

    • NAID

      110009838761

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of soil microorganisms on arsenite oxidation in paddy soils under oxic conditions2014

    • Author(s)
      D. T. Dong, N. Yamaguchi, T. Makino and S. Amachi
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 印刷中

    • NAID

      110009907896

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microbiology of inorganic arsenic: From metabolism to bioremediation2014

    • Author(s)
      S. Yamamura and S. Amachi
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 印刷中

    • NAID

      110009838761

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arsenic release from soil by a novel arsenate-reducing bacterium Anaeromyxobacter sp2013

    • Author(s)
      K. Kudo, N. Yamaguchi, T. Makino, T. Ohtsuka, K. Kimura. D. T. Dong and S. Amachi
    • Journal Title

      PSR-1, Appl. Environ. Microbiol

      Volume: 79 Pages: 4635-4642

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arsenic dissolution from Japanese paddy soil by a dissimilatory arsenate-reducing bacterium Geobacter sp2013

    • Author(s)
      T. Ohtsuka, N. Yamaguchi, T. Makino, K. Sakurai, K. Kimura, K. Kudo, E. Homma, D.T. Dong and S. Amachi
    • Journal Title

      OR-1, Environ. Sci. Technol

      Volume: 47 Pages: 6263-6271

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arsenic dissolution from Japanese paddy soil by a dissimilatory arsenate-reducing bacterium Geobacter sp. OR-12013

    • Author(s)
      T. Ohtsuka, N. Yamaguchi, T. Makino, K. Sakurai, K. Kimura, K. Kudo, E. Homma, D.T. Dong and S. Amachi
    • Journal Title

      Environmental Science and Technology

      Volume: 47 Issue: 12 Pages: 6263-6271

    • DOI

      10.1021/es400231x

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arsenic release from soil by a novel arsenate-reducing bacterium Anaeromyxobacter sp. PSR-12013

    • Author(s)
      K. Kudo, N. Yamaguchi, T. Makino, T. Ohtsuka, K. Kimura. D. T. Dong and S. Amachi
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 79 Issue: 15 Pages: 4635-4642

    • DOI

      10.1128/aem.00693-13

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arsenic release from flooded paddy soils is influenced by speciation, Eh, pH, and iron dissolution2011

    • Author(s)
      N. Yamaguchi, T. Nakamura, D. Dong, Y. Takahashi, S. Amachi and T. Makino
    • Journal Title

      Chemosphere

      Volume: 83 Pages: 925-932

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of soil microorganisms on arsenite oxidation in paddy soils under oxic conditions

    • Author(s)
      D. T. Dong, N. Yamaguchi, T. Makino and S. Amachi
    • Journal Title

      Soil Sci. Plant Nutr

      Volume: (in press)

    • NAID

      110009907896

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 異化的ヒ酸還元細菌Geobacter sp. OR-1株のドラフトゲノム解析2013

    • Author(s)
      江原彩香、天知誠吾
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2013-11-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Arsenic dissolution from Japanese paddy soil by a dissimilatory arsenate-reducing bacterium Geobacter sp2013

    • Author(s)
      S. Amachi, D. T. Dong, and N. Yamaguchi
    • Organizer
      OR-1, Goldschmidt 2013
    • Place of Presentation
      Florence, Italy
    • Year and Date
      2013-08-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 新規ヒ酸還元細菌の分離:ヒ素の還元溶出へ与えるインパクト2013

    • Author(s)
      天知誠吾
    • Organizer
      第1回Metal(loid) Biogeochemistry勉強会
    • Place of Presentation
      千葉大学
    • Year and Date
      2013-06-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Arsenic dissolution from Japanese paddy soil by a dissimilatory arsenate-reducing bacterium Geobacter sp. OR-12013

    • Author(s)
      S. Amachi, D. T. Dong, and N. Yamaguchi
    • Organizer
      Goldschmidt2013
    • Place of Presentation
      Florence, Italy
    • Related Report
      2013 Annual Research Report
  • [Presentation] 異化的ヒ酸還元細菌Geobacter sp. OR-1株のドラフトゲノム解析2013

    • Author(s)
      江原彩香、天知誠吾
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島大学、鹿児島
    • Related Report
      2013 Annual Research Report
  • [Presentation] 国内土壌より分離された亜ヒ酸酸化細菌KGO-5株の生理的特質2013

    • Author(s)
      董 旦、天知誠吾
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島大学、鹿児島
    • Related Report
      2013 Annual Research Report
  • [Presentation] 異化的ヒ酸還元能を有するAnaeromyxobacter sp. PSR-1株の性質2012

    • Author(s)
      工藤桂太郎、天知誠吾
    • Organizer
      第18回ヒ素シンポジウム
    • Place of Presentation
      宮日ホール
    • Year and Date
      2012-11-24
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒ酸還元能を有する鉄還元細菌Geobacter sp. OR-1株の生理的特質2012

    • Author(s)
      本間瑛理、天知誠吾
    • Organizer
      第18回ヒ素シンポジウム
    • Place of Presentation
      宮日ホール
    • Year and Date
      2012-11-24
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒ素呼吸能を持つ新規Geobacter属細菌による水田土壌からのヒ素の溶出2012

    • Author(s)
      天知誠吾
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋技術科学大学
    • Year and Date
      2012-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒ酸還元能を有する鉄還元細菌Geobacter sp. OR-1株の生理的特質2012

    • Author(s)
      本間瑛理、天知誠吾
    • Organizer
      第18回ヒ素シンポジウム
    • Place of Presentation
      宮崎市・宮日ホール
    • Related Report
      2012 Research-status Report
  • [Presentation] 異化的ヒ酸還元能を有するAnaeromyxobacter sp. PSR-1株の性質2012

    • Author(s)
      工藤桂太郎、天知誠吾
    • Organizer
      第18回ヒ素シンポジウム
    • Place of Presentation
      宮崎市・宮日ホール
    • Related Report
      2012 Research-status Report
  • [Presentation] 国内土壌より分離された亜ヒ酸酸化細菌KGO-5株2012

    • Author(s)
      董 旦、天知誠吾
    • Organizer
      第18回ヒ素シンポジウム
    • Place of Presentation
      宮崎市・宮日ホール
    • Related Report
      2012 Research-status Report
  • [Presentation] 水田土壌におけるヒ素の酸化および吸着に及ぼす微生物の影響2012

    • Author(s)
      董典涛、山口紀子、牧野知之、天知誠吾
    • Organizer
      第18回ヒ素シンポジウム
    • Place of Presentation
      宮崎市・宮日ホール
    • Related Report
      2012 Research-status Report
  • [Presentation] 国内水田土壌からのヒ素の溶出に及ぼす微生物の影響2011

    • Author(s)
      天知誠吾、中村崇志、大塚俊彦、櫻井和宏、木村建太、工藤桂太郎、牧野知之、山口紀子
    • Organizer
      第17回ヒ素シンポジウム
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2011-11-19
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] 国内水田土壌からの異化的ヒ酸還元細菌の分離2011

    • Author(s)
      工藤桂太郎、天知誠吾、本間瑛理
    • Organizer
      第27回日本微生物生態学会大会
    • Place of Presentation
      京都大学
    • Year and Date
      2011-10-08
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] Microbial influences on arsenic release from Japanese paddy soils2011

    • Author(s)
      S. Amachi, S. Nakamura, T. Ohtsuka, K. Kimura, K. Sakurai, K. Kudo, T. Makino and N. Yamaguchi
    • Organizer
      IUMS2011 Sapporo
    • Place of Presentation
      Sapporo
    • Year and Date
      2011-09-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Microbial influences on arsenic release from Japanese paddy soils2011

    • Author(s)
      S. Amachi, S. Nakamura, T. Ohtsuka, K. Kimura, K. Sakurai, K. Kudo, T. Makino and N. Yamaguchi
    • Organizer
      IUMS2011 Sapporo
    • Place of Presentation
      札幌国際会議場
    • Related Report
      2011 Research-status Report
  • [Book] 地球を救うメタルバイオロジー微生物と金属資源のはなし2014

    • Author(s)
      山村茂樹、天知誠吾
    • Publisher
      成山堂書店
    • Related Report
      2013 Final Research Report
  • [Book] 地球を救うメタルバイオロジー2014

    • Author(s)
      山村茂樹、天知誠吾
    • Total Pages
      160
    • Publisher
      成山堂書店
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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