Mechanism of selective transport in the retrograde traffic in the Golgi apparatus

• Project/Area Number: 23580105
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: NODA YOICHI 東京大学, 農学生命科学研究科, 助教 (90282699)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 微生物機能 / 小胞輸送 / ゴルジ体 / ER / 出芽酵母 / 蛋白質局在 / 局在 / 脂質 / 膜蛋白質

Research Abstract

It is an essential step for cells that newly synthesized proteins are correctly targeted to the organelles where they function. We performed genetical and biochemical experiments to reveal the mechanisms for selective membrane transport between ER-Golgi using Saccharomyces cerevisiae, an excellent model eukaryotic cell. We identified a new cargo for the Erv41-Erv46 complex and found an ER-Golgi recycling membrane protein whose precise function was previously unknown as a new candidate of a cargo receptor protein.

Research Products

• [Journal Article] Distinct adaptor proteins assist exit of Kre2-family proteins from the yeast ER (2014)
  • Author(s): Yoichi Noda, Takehiro Hara, Minako Ishii, and Koji Yoda
  • Journal Title: Biology Open Volume: 3 Issue: 3 Pages: 209-224
  • DOI: 10.1242/bio.20146312
  • Peer Reviewed
• [Journal Article] Molecular Mechanisms of the Localization of Membrane Proteins in the Yeast Golgi Compartments (2013)
  • Author(s): Yoichi Noda and Koji Yoda
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 3 Pages: 435-445
  • DOI: 10.1271/bbb.120982
  • NAID: 10031164760
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] 出芽酵母細胞壁β-1,6-グルカン合成の謎を解く (2013)
  • Author(s): 野田陽一,依田幸司
  • Journal Title: 化学と生物 Volume: Vol. 51, No. 6 Pages: 383-388
  • NAID: 10031177625
• [Journal Article] 出芽酵母細胞壁β-1,6-グルカン合成の謎を解く (2013)
  • Author(s): 野田陽一，依田幸司
  • Journal Title: 化学と生物 Volume: 51 Pages: 383-388
  • NAID: 10031177625
  • Peer Reviewed
• [Journal Article] Action of multiple endoplasmic reticulum chaperon-like proteins is required for proper folding and polarized localization of Kre6 protein essential in yeast cell wallβ-1, 6-glucan synthesis (2012)
  • Author(s): Tomokazu Kurita, Yoichi Noda and Koji Yoda.
  • Journal Title: Journal of Biological Chemistry Volume: 287(21) Issue: 21 Pages: 17415-17424
  • DOI: 10.1074/jbc.m111.321018
  • Peer Reviewed
• [Journal Article] Kre6 protein essential for yeast cell-wallβ-1, 6-glucan synthesis accumulates at sites of polarized growth (2011)
  • Author(s): Tomokazu Kurita, Yoichi Noda, Tomoko Takagi, Masako Osumi, and Koji Yoda.
  • Journal Title: Journal of Biological Chemistry Volume: 286(9) Issue: 9 Pages: 7429-7438
  • DOI: 10.1074/jbc.m110.174060
  • Peer Reviewed
• [Presentation] 出芽酵母小胞体からの膜タンパク質の選択的輸送機構の解析 (2014)
  • Author(s): 藤井聖也,野田陽一,依田幸司
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 東京
• [Presentation] 出芽酵母細胞壁成分β-1,6-グルカン合成関連タンパク質Kre9の解析 (2014)
  • Author(s): 林田光弘,野田陽一,依田幸司
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 東京
• [Presentation] 出芽酵母の糖鎖修飾に関わる膜蛋白質のオルガネラ局在化機構 (2013)
  • Author(s): 野田陽一,藤井聖也,依田幸司
  • Organizer: 酵母細胞研究会第184回例会
  • Place of Presentation: 東京
  • Year and Date: 2013-07-12
  • Invited
• [Presentation] The Effect of multiple disruption of genes encoding yeast COPII vesicle proteins on the organelle localization of membrane proteins (2013)
  • Author(s): 藤井聖也,野田陽一,依田幸司
  • Organizer: 第13回東京大学生命科学シンポジウム
  • Year and Date: 2013-06-08
• [Presentation] Analysis of the Kre9 protein involved in the biosynthesis of the cell wallβ-1,6-glucan in Saccharomyces cerevisiae (2013)
  • Author(s): 林田光弘,野田陽一,依田幸司
  • Organizer: 第13回東京大学生命科学シンポジウム
  • Year and Date: 2013-06-08
• [Presentation] 出芽酵母COPII小胞膜タンパク質遺伝子の多重欠損株におけるゴルジ体膜タンパク質の局在解析 (2013)
  • Author(s): 藤井聖也,野田陽一,依田幸司
  • Organizer: 2013年度日本農芸化学会大会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2013-03-25
• [Presentation] Distinct Adaptor Proteins Assist ER Exit of Yeast Kre2-family and related Membrane Proteins Involved in Protein Mannosylation (2013)
  • Author(s): Yoichi Noda and Koji Yoda
  • Organizer: American Society for Cell Biology Annual Meeting 2013
  • Place of Presentation: Neworleans, USA
• [Presentation] 出芽酵母におけるマンノース転移酵素のオルガネラ局在化機構 (2013)
  • Author(s): 野田陽一
  • Organizer: 第86回生化学会大会
  • Place of Presentation: 横浜
  • Invited
• [Presentation] 出芽酵母細胞壁成分β-1,6-glucan合成関連蛋白質Kre9の解析 (2013)
  • Author(s): 林田光弘,野田陽一,依田幸司
  • Organizer: 酵母遺伝学フォーラム第46回研究報告会
  • Place of Presentation: 仙台
• [Presentation] 出芽酵母細胞壁β-1,6-glucanの合成に関わる遺伝子KRE5, KRE6の解析 (2013)
  • Author(s): 小西俊彦,野田陽一,依田幸司
  • Organizer: 酵母遺伝学フォーラム第46回研究報告会
  • Place of Presentation: 仙台
• [Presentation] 出芽酵母COPII小胞膜タンパク質遺伝子の多重欠損株におけるゴルジ体膜タンパク質の局在解析 (2013)
  • Author(s): 藤井聖也,,野田陽一,依田幸司
  • Organizer: 酵母遺伝学フォーラム第46回研究報告会
  • Place of Presentation: 仙台
• [Presentation] Distinct Adaptor Proteins Assist ER Exit of Yeast Kre2-family and related Proteins Involved in Protein Mannosylation (2013)
  • Author(s): Yoichi Noda and Koji Yoda
  • Organizer: 26th International Conference on Yeast Genetics and Molecular Biology
  • Place of Presentation: Frankfurt/M. Germany
• [Presentation] ER membrane protein Keg1 is essential for yeast cell-wallβ-1,6-glucan synthesis through folding and functional localization of Kre6 and Skn1 (2013)
  • Author(s): Koji Yoda and Yoichi Noda
  • Organizer: 30th INTERNATIONAL SPECIALIZED SYMPOSIUM ON YEAST (ISSY2013)"Cell Surface and Organelles in Yeasts : from Basics to Applications"
  • Place of Presentation: Stara Lesna, Slovakia
• [Presentation] Distinct Adaptor Proteins Assist ER Exit of Yeast Kre2-family proteins to localize in the Golgi (2013)
  • Author(s): Yoichi Noda and Koji Yoda
  • Organizer: 30th INTERNATIONAL SPECIALIZED SYMPOSIUM ON YEAST (ISSY2013)"Cell Surface and Organelles in Yeasts : from Basics to Applications"
  • Place of Presentation: Stara Lesna, Slovakia
• [Presentation] ER membrane protein Keg1 is essential for yeast cell-wall β-1,6-glucan synthesis through folding and functional localization of Kre6 and Skn1 (2013)
  • Author(s): Koji Yoda and Yoichi Noda
  • Organizer: 30th INTERNATIONAL SPECIALIZED SYMPOSIUM ON YEAST (ISSY2013)"Cell Surface and Organelles in Yeasts: from Basics to Applications"
  • Place of Presentation: Stara Lesna, Slovakia
• [Presentation] Distinct Adaptor Proteins Assist ER Exit of Yeast Kre2-family proteins to localize in the Golgi (2013)
  • Author(s): Yoichi Noda and Koji Yoda
  • Organizer: 30th INTERNATIONAL SPECIALIZED SYMPOSIUM ON YEAST (ISSY2013)"Cell Surface and Organelles in Yeasts: from Basics to Applications"
  • Place of Presentation: Stara Lesna, Slovakia
• [Presentation] 出芽酵母細胞壁成分 β-1,6-glucan 合成関連蛋白質Kre9の解析 (2013)
  • Author(s): 林田光弘，野田陽一，依田幸司
  • Organizer: 酵母遺伝学フォーラム第46回研究報告会
  • Place of Presentation: 仙台
• [Presentation] 出芽酵母細胞壁 β-1,6-glucanの合成に関わる遺伝子KRE5, KRE6の解析 (2013)
  • Author(s): 小西俊彦，野田陽一，依田幸司
  • Organizer: 酵母遺伝学フォーラム第46回研究報告会
  • Place of Presentation: 仙台
• [Presentation] 出芽酵母N糖鎖のマンノースリン酸付加に必要なMnn4蛋白のゴルジ体局在はSvp26とMnn6蛋白質に依存する (2012)
  • Author(s): 野田陽一,依田幸司
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡マリンメッセ(福岡県)
  • Year and Date: 2012-12-16
• [Presentation] 出芽酵母N糖鎖のマンノースリン酸の付加に関与するMnn4蛋白のゴルジ体局在はSvp26とMnn6蛋白質に依存する (2012)
  • Author(s): 野田陽一,依田幸司
  • Organizer: 第45回酵母遺伝学フォーラム研究報告会
  • Place of Presentation: 京都大学宇治キャンパス(京都府)
  • Year and Date: 2012-09-06
• [Presentation] A Novel localization mechanism of yeast mannosyltransferases in the Golgi (2012)
  • Author(s): Yoichi Noda and Koji Yoda
  • Organizer: V INTERNATIONAL CONFERENCE ON MOLECULAR MECHANISMS OF FUNGAL CELL WALL BIOGENESIS
  • Place of Presentation: Primosten, Hotel Zora, Croatia
  • Year and Date: 2012-06-06
• [Presentation] 出芽酵母の細胞壁多糖、β-1,6-グルカンの合成に必須のKre6が機能を発揮するにはERの複数のシャペロン様タンパク質の働きが必要である (2012)
  • Author(s): 栗田朋和,野田陽一,依田幸司
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学(京都府)
  • Year and Date: 2012-03-25
• [Presentation] 出芽酵母ゴルジ体マンノース転移酵素の小胞体搬出を促進する蛋白質の機能解析 (2011)
  • Author(s): 野田陽一,石井美奈子,依田幸司
  • Organizer: 日本生化学会2011年度大会
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2011-09-22
• [Presentation] 出芽酵母Svp26蛋白質はゴルジ体マンノース転移酵素Ktr1, Kre2の小胞体搬出アダプターとして機能する (2011)
  • Author(s): 野田陽一,依田幸司
  • Organizer: 酵母遺伝学フォーラム第44回研究会
  • Place of Presentation: 九州大学医学部(福岡県)
  • Year and Date: 2011-09-05
• [Presentation] β-1,6-グルカン合成に必須のKre6の折り畳みと搬出にはERのシャペロン様タンパク質が必要である (2011)
  • Author(s): 栗田朋和,野田陽一,依田幸司
  • Organizer: 酵母遺伝学フォーラム第44回研究会
  • Place of Presentation: 九州大学医学部(福岡県)
  • Year and Date: 2011-09-05
• [Presentation] β-1,6-グルカン合成に必須のKre６の折り畳みと搬出にはERのシャペロン様タンパク質が必要である (2011)
  • Author(s): 栗田朋和，野田陽一，依田幸司
  • Organizer: 酵母遺伝学フォーラム第44回研究会
  • Place of Presentation: 九州大学医学部(福岡県)
• [Presentation] 出芽酵母N糖鎖のマンノースリン酸の付加に関与するMnn4蛋白のゴルジ体局在はSvp26とMnn6蛋白質に依存する
  • Author(s): 野田陽一，依田幸司
  • Organizer: 第45回酵母遺伝学フォーラム研究報告会
  • Place of Presentation: 京都大学宇治キャンパス（京都府）
• [Presentation] 出芽酵母N糖鎖のマンノースリン酸付加に必要なMnn4蛋白のゴルジ体局在はSvp26とMnn6蛋白質に依存する
  • Author(s): 野田陽一，依田幸司
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡マリンメッセ（福岡県）
• [Presentation] 出芽酵母COPII小胞膜タンパク質遺伝子の多重欠損株におけるゴルジ体膜タンパク質の局在解析
  • Author(s): 藤井聖也，野田陽一，依田幸司
  • Organizer: 2013年度日本農芸化学会大会
  • Place of Presentation: 東北大学（宮城県）
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/molbiotech/ttp://www.bt.a.u-tokyo.ac.jp/_kougaku/01/main200.html