| Project/Area Number |
23580106
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Nagaoka University of Technology |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | D-アミノ酸オキシダーゼ / 耐熱性酵素 / 好熱菌 / Rubrobacter xylanophilus / D-アミノ酸 / 生体触媒 / フラビン酵素 / オキシダーゼ / DAO |
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| Research Abstract |
D-Amino acid oxidase (DAO) is a biotechnologically important enzyme that can be used for various applications, such as the determination of D-amino acids and the production of pharmaceutical intermediates. Therefore, a highly stable DAO has been desired.
In this study, we identified a DAO homologous gene (RxDAO) in the thermophilic bacterium Rubrobacter xylanophilus, and showed that the gene product produced in E. coli has a much higher stability than known DAOs. In addition, we identified DAO homologous genes in another thermophilic bacterium and also in a thermophilic fungus, showing the possibility of the additional existence of other highly stable DAOs. In conclusion, we successfully identified a novel, highly stable DAO useful for practical applications in various industrial fields.
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