Expression level of methanol-inducible peroxisomal proteins is affected by oxygen conditions and mitochondrial respiratory pathway function in the methylotrophic yeast
| Project/Area Number |
23580109
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Gifu University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | メチロトローフ酵母 / 酸素認識 / アルコールオキシダーゼ / 呼吸鎖 / ペルオキシソーム / methylotrophic yeast / oxygen / alcohol oxidase / Candida boidinii / peroxisome / Pichia pastoris / oxygen recognition / CYC1 / gene expression |
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| Research Abstract |
Oxygen is required by methylotrophic yeast for the AOD-catalyzed reactions within peroxisomes for methanol metabolism in addition to its role as the terminal electron acceptor for the respiratory chain in the mitochondria. Therefore, the yeast cells must have a mechanism for controlling the methanol metabolism, which responds to oxygen levels, in order to balance the oxygen needs between these two organelles. In this study we showed that methanol-inducible peroxisomal proteins were induced only under aerobic conditions, and those gene expression was repressed by inhibition of the mitochondrial respiratory chain. In the respiratory deficient mutant strains, their induction was at very low levels despite the presence of oxygen. Taken together, these facts indicate that the yeast can sense oxygen conditions, and that mitochondrial respiratory function may have a profound effect on induction of methanol-inducible gene expression of peroxisomal proteins.
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Report
(4 results)
Research Products
(18 results)
