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Expression level of methanol-inducible peroxisomal proteins is affected by oxygen conditions and mitochondrial respiratory pathway function in the methylotrophic yeast

Research Project

Project/Area Number 23580109
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionGifu University

Principal Investigator

NAKAGAWA Tomoyuki  岐阜大学, 応用生物科学部, 教授 (70318179)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsメチロトローフ酵母 / 酸素認識 / アルコールオキシダーゼ / 呼吸鎖 / ペルオキシソーム / methylotrophic yeast / oxygen / alcohol oxidase / Candida boidinii / peroxisome / Pichia pastoris / oxygen recognition / CYC1 / gene expression
Research Abstract

Oxygen is required by methylotrophic yeast for the AOD-catalyzed reactions within peroxisomes for methanol metabolism in addition to its role as the terminal electron acceptor for the respiratory chain in the mitochondria. Therefore, the yeast cells must have a mechanism for controlling the methanol metabolism, which responds to oxygen levels, in order to balance the oxygen needs between these two organelles. In this study we showed that methanol-inducible peroxisomal proteins were induced only under aerobic conditions, and those gene expression was repressed by inhibition of the mitochondrial respiratory chain. In the respiratory deficient mutant strains, their induction was at very low levels despite the presence of oxygen. Taken together, these facts indicate that the yeast can sense oxygen conditions, and that mitochondrial respiratory function may have a profound effect on induction of methanol-inducible gene expression of peroxisomal proteins.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (18 results)

All 2014 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (15 results)

  • [Journal Article] Expression level of methanol-inducible peroxisomal proteins and peroxisome morphology are affected by oxygen conditions and mitochondrial respiratory pathway function in the methylotrophic yeastCandida boidinii.2013

    • Author(s)
      S. Fujimura, H. Yurimoto, S. Kurimoto, Y.Matsufuji, T. Ito, T. Hayakawa, N. Tomizuka, Y. Sakai, and T. Nakagawa
    • Journal Title

      FEMS Yeast Res

      Volume: 13 Issue: 4 Pages: 359-366

    • DOI

      10.1111/1567-1364.12040

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 酵母のメタノール代謝制御の分子メカニズムの解明とその応用2012

    • Author(s)
      中川智行
    • Journal Title

      生物工学会誌

      Volume: 90 Pages: 72-77

    • NAID

      110009419608

    • URL

      https://www.sbj.or.jp/wp-content/uploads/file/sbj/9002/9002_saito.pdf

    • Related Report
      2013 Final Research Report
  • [Journal Article] 酵母のメタノール代謝制御の分子メカニズムの解明とその応用(総説)2012

    • Author(s)
      中川智行
    • Journal Title

      生物工学会誌

      Volume: 90 Pages: 72-77

    • Related Report
      2011 Research-status Report
  • [Presentation] メチロトローフ酵母Pichia methanolicaの様々な炭素源による生育特性とAODアイソザイムの発現誘導2014

    • Author(s)
      若山敬嗣、早川享志、中川智行
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学川崎
    • Year and Date
      2014-03-30
    • Related Report
      2013 Final Research Report
  • [Presentation] メチロトローフ酵母Pichia methanolicaの様々な炭素源による生育特性とAODアイソザイムの発現誘導2014

    • Author(s)
      若山 敬嗣,早川 享志,中川 智行
    • Organizer
      日本農芸化学会 2014年度大会
    • Place of Presentation
      明治大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] メチロトローフ酵母Pichia pastoris AOX1の酸素応答におけるCyc1pの役割2013

    • Author(s)
      岡田一真、栗本将太、早川享志、中川智行
    • Organizer
      日本農芸化学会中部支部168回例会
    • Place of Presentation
      名古屋大学名古屋
    • Year and Date
      2013-10-12
    • Related Report
      2013 Final Research Report
  • [Presentation] メチロトローフ酵母Pichia methanolicaの異種遺伝子発現系への応用を目指した新たな炭素源の探索とAODアイソザイムの発現特性の解析2013

    • Author(s)
      若山敬嗣、早川享志、中川智行
    • Organizer
      日本農芸化学会中部支部168回例会
    • Place of Presentation
      名古屋大学名古屋
    • Year and Date
      2013-10-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Oxygen conditions and mitochondrial respiratory function influence expression level of methanol-inducible peroxisomal proteins and peroxisome morphology in the methylotrophic yeast. BioMicroWorld 2013: 5th International Conference on Environmental2013

    • Author(s)
      Nakagawa T, Fujimura S, Yurimoto H, Sakai Y and Hayakawa T.
    • Organizer
      Industrial and Applied Microbiology
    • Place of Presentation
      マドリードスペイン
    • Year and Date
      2013-10-03
    • Related Report
      2013 Final Research Report
  • [Presentation] メチロトローフ酵母Pichia pastorisシトクロムcの発現制御とメタノール代謝における役割2013

    • Author(s)
      岡田一真、栗本将太、早川享志、中川智行
    • Organizer
      日本農芸化学会2013年大会
    • Place of Presentation
      東北大学仙台
    • Year and Date
      2013-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] メチロトローフ酵母Pichia pastorisシトクロムcの発現制御とメタノール代謝における役割2013

    • Author(s)
      岡田 一真、栗本 将太、早川 享志、中川 智行
    • Organizer
      日本農芸化学会 2013年度大会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Regulation of methanol metabolism in the methylotrophic yeast2011

    • Author(s)
      Nakagawa T.
    • Organizer
      2011 KSBB Fall Meeting & International Symposium
    • Place of Presentation
      仁川大韓民国
    • Year and Date
      2011-10-06
    • Related Report
      2013 Final Research Report
  • [Presentation] 酵母のメタノール代謝制御の分子メカニズムの解明とその応用2011

    • Author(s)
      中川智行
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学東京
    • Year and Date
      2011-09-26
    • Related Report
      2013 Final Research Report
  • [Presentation] メチロトローフ酵母Pichia pastoris AOX1の酸素応答におけるシトクロムcの役割2011

    • Author(s)
      栗本将太、早川享志、中川智行
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学東京
    • Year and Date
      2011-09-26
    • Related Report
      2013 Final Research Report
  • [Presentation] メチロトローフ酵母Pichia pastoris AOX1の酸素応答におけるシトクロムcの役割2011

    • Author(s)
      栗本将太、早川享志、中川智行
    • Organizer
      第63回 日本生物工学会 大会
    • Place of Presentation
      東京農工大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 酵母のメタノール代謝制御の分子メカニズムの解明とその応用2011

    • Author(s)
      中川智行
    • Organizer
      第63回 日本生物工学会 大会(招待講演)
    • Place of Presentation
      東京農工大学
    • Related Report
      2011 Research-status Report
  • [Presentation] Regulation of methanol metabolism in yeast2011

    • Author(s)
      Tomoyuki Nakagawa
    • Organizer
      2011 KSBB Fall Meeting&International Symposium
    • Place of Presentation
      Songdo Convensia, Incheon, Korea
    • Related Report
      2011 Research-status Report
  • [Presentation] メチロトローフ酵母Pichia pastoris AOX1の酸素応答におけるCyc1pの役割

    • Author(s)
      岡田 一真,栗本 将太,早川 享志,中川 智行
    • Organizer
      日本農芸化学会 中部支部 第168回 例会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] メチロトローフ酵母Pichia methanolicaの異種遺伝子発現系への応用を目指した新たな炭素源の探索とAODアイソザイムの発現特性の解析

    • Author(s)
      若山 敬嗣,早川 享志,中川 智行
    • Organizer
      日本農芸化学会 中部支部 第168回 例会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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