| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
A part of reactions in the archaeal mevalonate pathways were unidentified. Thus we performed the conversion assay of radio-labeled substrates by cell-free extract from archaea and functional characterization of archaeal homologs of known mevalonate pathway-related enzymes. As the result, we discovered the classical mevalonate pathway, which is rare in archaea, from an archaeon of genus Sulfolobus, and a novel modified pathway, which passes through 3-phosphomevalonate, from an archaeon of genus Thermoplasma.
We also performed X-ray crystal structural studies on type 2 isopentenyl diphosphate isomerase and diphosphomevalonate decarboxylase from archaea of genus Sulfolobus. The obtained structures and the results of various analyses showed novel mechanisms of catalysis, regulation of activity, and thermostabilization.
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