Generic technology for halophilic bacteria using genomic data from next genearation sequencer
| Project/Area Number |
23580117
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | University of the Ryukyus |
|---|
Principal Investigator |
MATSUI Toru 琉球大学, 熱帯生物圏研究センター, 准教授 (90372812)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
|---|
| Keywords | 好塩性細菌 / オキシゲナーゼ / シデロフォア / P450 / 炭化水素 / 次世代シーケンサー |
|---|
| Research Abstract |
Generic technology for moderately halophilic bacteria was examined using isolates from various area of Tunisia in order to apply for bioremediation under salinity conditions. Screening conditions for the variety of halophiles, genome analysis using next generation sequencer, plasmid vector construction with transformation, gene disruptant library construction, and collection of petroleum related compounds degradative genes were examined. Construction of petroleum degradation bacteria working under a salinity condition using the above materials is under progress.
|
Report
(4 results)
Research Products
(9 results)
