Generic technology for halophilic bacteria using genomic data from next genearation sequencer

• Project/Area Number: 23580117
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: University of the Ryukyus
• Principal Investigator: MATSUI Toru 琉球大学, 熱帯生物圏研究センター, 准教授 (90372812)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 好塩性細菌 / オキシゲナーゼ / シデロフォア / P450 / 炭化水素 / 次世代シーケンサー

Research Abstract

Generic technology for moderately halophilic bacteria was examined using isolates from various area of Tunisia in order to apply for bioremediation under salinity conditions. Screening conditions for the variety of halophiles, genome analysis using next generation sequencer, plasmid vector construction with transformation, gene disruptant library construction, and collection of petroleum related compounds degradative genes were examined. Construction of petroleum degradation bacteria working under a salinity condition using the above materials is under progress.

Research Products

• [Journal Article] High cell density cultivation of Pseudomonas putida strain HKT554 and its application for optically active sulfoxide production (2013)
  • Author(s): S. H. Ramadhan, T. Matsui, K. Nakano, H. Minami
  • Journal Title: Appl. Microbiol. Biotechnol Volume: 97 Pages: 1903-1907
  • Peer Reviewed
• [Journal Article] High cell density cultivation of Pseudomonas putida strain HKT554 and its application for optically active sulfoxide production. (2013)
  • Author(s): Said Hamad Ramadhan, Toru Matsui, Kazuma Nakano, Hirofumi Minami
  • Journal Title: Applied Microbiology and Biotechnology Volume: 97 Issue: 5 Pages: 1903-1907
  • DOI: 10.1007/s00253-012-4445-x
  • Peer Reviewed
• [Presentation] Halophilic bacteria isolated from Tunisia and Okinawa.,Tunisia-Japan Symposium on Society (2013)
  • Author(s): T.Matsui, T.Uezu, L. El Bassi, N. Nugara, H. Abdennaceur, H. Isoda
  • Organizer: Science and Technology
  • Place of Presentation: Hammamet, Tunisia
• [Presentation] E.coli-Halomonas sp.シャトルベクターの構築とその性質 (2013)
  • Author(s): 松井徹, Nilushi Nugara,東江菜都美,上江洲敏子,磯田博子
  • Organizer: 生物工学会
  • Place of Presentation: 広島
• [Presentation] E.coli-Halomonas sp. シャトルベクターの構築とその性質 (2013)
  • Author(s): 松井 徹, Nilushi Nugara 東江菜都美, 上江洲敏子, 磯田博子
  • Organizer: 日本生物工学会
  • Place of Presentation: 広島国際会議場
• [Presentation] Halophilic bacteria isolated from Tunisia and Okinawa. (2013)
  • Author(s): T.Matsui, T.Uezu, L. El Bassi, N. Nugara, H. Abdennaceur, H. Isoda
  • Organizer: Tunisia-Japan Symposium on Society, Science and Technology
  • Place of Presentation: Hammamet, Tunisia
• [Presentation] チュニジア環境試料からの好塩性細菌分離と454GSJrによるドラフトゲノム解析 (2012)
  • Author(s): 松井徹、青山洋昭、斎藤星耕、新里尚也、松崎吾朗、磯田博子、Hassen Abd-Ennasser、Nilushi Nugara
  • Organizer: 農芸化学会
  • Place of Presentation: 京都
• [Presentation] チュニジア環境試料からの好塩性細菌分離と454GSJrによるドラフトゲノム解析
  • Author(s): 松井 徹
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都女子大学
• [Presentation] High Cell Density Cultivation of Microorganisms
  • Author(s): 松井 徹
  • Organizer: Tunisia-Japan Symposium on Society, Science and Technology
  • Place of Presentation: "Le Royal" Hotel-HAMMAMETHammamet, Tunisia