Elucidation of the molecular mechanism of lipase super-induction and creation of high stereo-selective lipase.
| Project/Area Number |
23580119
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Chuo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
AKANUMA Genki 中央大学, 理工学部, 助教 (30580063)
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| Co-Investigator(Renkei-kenkyūsha) |
USHIO Kazutoshi 新居浜工業高等専門学校, 生物応用化学科, 教授 (30203508)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | 脂質代謝 / リパーゼ / リパーゼ超誘導剤 / 高級アルコール / 界面活性タンパク質 / ポリエステル / プロテオミクス / タンパク質工学 / リパーゼ・スーパーインデューサー / 結晶化 / ポリエステルの蓄積 / プロテオーム解析 / 脂質 / 発現制御 / シグナル伝達 / 生体機能利用 |
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| Research Abstract |
Stable and high stereo-selective lipase has attracted attention from the viewpoint of industrial usage. As compared with the case where it was olive growing, the addition of stearyl alcohol resulted in enhanced several tens folds of thermo-stable and high stereo-selective lipase activity. Not only lipase but also surface active protein (EliA) was strongly induced in a large amount and secreted. From our experimental results, We can propose a model of lipase super-production system as follows: uptake of a small amount of lipase super-inducer, induction of EliA and secretion, emulsification of stearyl alcohol by EliA, uptake of a large amount of lipase super-inducer, and strong induction of lipase and secretion. It has become possible to obtain a large amount of high stereo-selective lipase by application of the mechanism.
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Report
(4 results)
Research Products
(66 results)
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[Journal Article] Single mutations introduced in the essential ribosomal proteins L3 and S10 cause sporulation defect in Bacillus subtilis2013
Author(s)
Genki Akanuma, Shota Suzuki, Koichi Yano, Hideaki Nanamiya, Yousuke Natori, Eri Namba, Kazuya Watanabe, Kazumi Tagami, Takuya Takeda, Yuka Iizuka, Ako Kobayashi, Morio Ishizuka, Hirofumi Yoshikawa, and Fujio Kawamura
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Journal Title
J. Gen. Appl. Microbiol
Volume: 59(2)
Pages: 105-117
NAID
Related Report
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[Journal Article] Single mutations introduced in the essential ribosomal proteins L3 and S10 cause a sporulation defect in <i>Bacillus subtilis</i>2013
Author(s)
Genki Akanuma, Shota Suzuki, Koichi Yano, Hideaki Nanamiya, Yousuke Natori, Eri Namba, Kazuya Watanabe, Kazumi Tagami, Takuya Takeda, Yuka Iizuka, Ako Kobayashi, Morio Ishizuka, Hirofumi Yoshikawa, and Fujio Kawamura
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Journal Title
The Journal of General and Applied Microbiology
Volume: 59
Issue: 2
Pages: 105-117
DOI
NAID
ISSN
0022-1260, 1349-8037
Related Report
Peer Reviewed
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[Journal Article] Single mutations introduced in the essential ribosomal proteins L3 and S10 cause sporulation defect in Bacillus subtilis2013
Author(s)
Genki Akanuma, S. Suzuki, K. Yano, H. Nanamiya, Y. Natori, E. Namba, K. Watanabe, K. Tagami, T. Takeda, Y. Iizuka, A. Kobayashi, Morio Ishizuka, H. Yoshikawa, and F. Kawamura
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Journal Title
J. Gen. Appl. Microbiol.
Volume: 未定
NAID
Related Report
Peer Reviewed
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[Presentation] Role of lipase super-inducers in co-super-production system of lipase and surface-active protein from Pseudomonas-like bacteria2012
Author(s)
Ishizuka, M., Ishibashi, H., Miyagawa, T., Osawa, R., Sekiya, M., Katagiri, R., Yoshizawa, R., Sato, N., Yamaguchi, M., Akanuma, G., and Ushio, K.
Organizer
World Congress on Oleo Science (WCOS 2012) & 29th ISF Congress–JOCS / AOCS / KOCS / ISF Joint Meeting
Place of Presentation
Sasebo, Japan
Related Report
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[Presentation] Molecular mechanism of co-super-production system of lipase and surface-active protein from Pseudomonas-like bacteria. FEBS Journal, 279 (sup.1), 348, 20122012
Author(s)
M. Ishizuka, H. Ishibashi, T. Miyagawa, R. Osawa, M. Sekiya, R. Katagiri, R. Yoshizawa, N. Sato, M. Yamaguchi, G. Akanuma and K. Ushio
Organizer
Special Issue of 22nd IUBMB & 37th FEBS Congress
Place of Presentation
Seville, Spain
Related Report
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[Presentation] Molecular mechanism of co-super-production system of lipase and surface-active protein from pseudomonas-like bacteria2012
Author(s)
M. Ishizuka, H. Ishibashi, T. Miyagawa, R. Osawa, M. Sekiya, R. Katagiri, R. Yoshizawa, N. Sato, M. Yamaguchi, G. Akanuma, and K. Ushio
Organizer
22nd IUBMB & 37th FEBS Congress
Place of Presentation
Seville, Spain
Related Report
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[Presentation] Role of lipase super-inducers in co-super-production system of lipase and surface-active protein from Pseudomonas-like bacteria2012
Author(s)
Ishizuka, M., Ishibashi, H., Miyagawa, T., Osawa, R., Sekiya, M., Katagiri, R., Yoshizawa, R., Sato, N., Yamaguchi, M., Akanuma, G., and Ushio, K.
Organizer
World Congress on Oleo Science (WCOS 2012) & 29th ISF Congress, JOCS/AOCS/KOCS/ISF Joint Meeting
Place of Presentation
Sasebo, Japan
Related Report
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[Presentation] Co-super- production system of lipase and surface- active protein from Pseudomonas-like bacteria. Cell Signaling Networks Conference (CSN2011) : 13th IUBMB Conference2011
Author(s)
Ishizuka, M., Ishibashi, H., Miyagawa, T., Akanuma, G., and Ushio, K.
Organizer
1st PABMB Conference and 3rd Meetings of the Signal Transduction Branch & Oxidative Stress Branches of SMB
Place of Presentation
Merida, Yucatan, Mexico
Related Report
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[Presentation] Co-super-production system of lipase and surface-active protein from Pseudomonas-like bacteria2011
Author(s)
Ishizuka, M., Ishibashi, H., Miyagawa, T., Akanuma, G., and Ushio, K.
Organizer
Cell Signaling Networks Conference (CSN2011)
Place of Presentation
Merida, Mexico
Related Report
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