| Project/Area Number |
23580119
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Chuo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
AKANUMA Genki 中央大学, 理工学部, 助教 (30580063)
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| Co-Investigator(Renkei-kenkyūsha) |
USHIO Kazutoshi 新居浜工業高等専門学校, 生物応用化学科, 教授 (30203508)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | 脂質代謝 / リパーゼ / リパーゼ超誘導剤 / 高級アルコール / 界面活性タンパク質 / ポリエステル / プロテオミクス / タンパク質工学 / リパーゼ・スーパーインデューサー / 結晶化 / ポリエステルの蓄積 / プロテオーム解析 / 脂質 / 発現制御 / シグナル伝達 / 生体機能利用 |
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| Research Abstract |
Stable and high stereo-selective lipase has attracted attention from the viewpoint of industrial usage. As compared with the case where it was olive growing, the addition of stearyl alcohol resulted in enhanced several tens folds of thermo-stable and high stereo-selective lipase activity. Not only lipase but also surface active protein (EliA) was strongly induced in a large amount and secreted. From our experimental results, We can propose a model of lipase super-production system as follows: uptake of a small amount of lipase super-inducer, induction of EliA and secretion, emulsification of stearyl alcohol by EliA, uptake of a large amount of lipase super-inducer, and strong induction of lipase and secretion. It has become possible to obtain a large amount of high stereo-selective lipase by application of the mechanism.
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