Construction of cell-free anammox reaction system
| Project/Area Number |
23580123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Sojo University |
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Principal Investigator |
FUJII Takao 崇城大学, 生物生命学部, 教授 (80165331)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIYAMA Takashi 崇城大学, 生物生命学部, 准教授 (00425331)
HIRA Daisuke 崇城大学, 生物生命学部, 助教 (00569890)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Keywords | 嫌気性アンモニア酸化 / 脱窒 / アナモックス / 亜硝酸還元酵素 / ヘムタンパク質 / ヒドラジン合成酵素 / ヒドロキシルアミン酸化還元酵素 / ヒドラジン / ヒドラジン酸化酵素 / anammox / ヒドラジン生合成 / ヒドラジン合成 / へムタンパク質 |
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| Research Abstract |
This study was performed to construct cell-free anaerobic reaction system by reference to the previously described model for mechanism of anammox. The following important findings for construction of the system were obtained. (1) Hydrazine was synthesized from NO and NH4+ catalyzed by hydrazine synthase with a heterodimer heme protein, NaxLS. (2) An intermediate, NO, was synthesized by Cu-type nitrite reductase (Cu-Nir) in strain KSU-1 although cytochrome cd1 Nir has only been known in other anammox bacteria. (3) A multiheme protein, hydroxylamine oxidoreductase (HAO), catalyzed inter-conversion of NO and hydroxylamine.
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Report
(4 results)
Research Products
(44 results)
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[Presentation] Hydrazine synthase from an Anammox Bacterium Strain KSU-12013
Author(s)
Hira, D., Okubo, H., Ichikawa, S., Furukawa, K., and Fujii, T.
Organizer
Second International Anammox Symposium
Place of Presentation
COEX, Seoul, Korea
Related Report
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[Patent(Industrial Property Rights)] ヒドラジン合成法2013
Inventor(s)
藤井隆夫、平大輔、大久保寛幹
Industrial Property Rights Holder
学校法人君が淵学園、藤井隆夫、 平大輔
Industrial Property Rights Type
特許
Industrial Property Number
2013-237127
Filing Date
2013-11-15
Related Report
Overseas
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