| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
We have constructed a pseudo-bacterial cell model using streptavidin coated fluorescent beads in conjugation with biotinylated capsular polysaccharides (CPS) purified from Lactococcus cells. The CPS-labeled beads were added to macrophage-like cell line J774.1 and cells that engulfed CPS-labeled beads were analyzed by flow cytometry. Ruthenium red-binding factor specific to the CPS of Lactococcus lactis C59 was purified and found to be RNAs, in which ribosome RNAs were the main components. By scanning electron microscopy, there were particle-like structures on the cell surface of C59 but no lesions from which RNAs were eluted. Particle-like structures were not observed on the surface of strain C63, a CPS-deficient C59 congenic strain. The result of next-generation sequencing showed that C59 and C63 possessed the identical eps gene cluster.
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