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Development a novel method to analyze blood vessel occurrence in metanephroi and its network construction

Research Project

Project/Area Number 23580129
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied biochemistry
Research InstitutionUniversity of Tsukuba

Principal Investigator

WANG Pichao  筑波大学, 生命環境系, 教授 (80261775)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsangiogenesis / vasculogenesis / 後腎 / 発生期 / 墨鋳型法 / 血管形成 / 血管鋳型 / 発生期後腎 / 血管新生 / 気液相培養 / 血管網構築 / 拍動流 / セラミックス多孔性担体 / 鋳型血管観察法 / 腎臓発生血管網 / 血管鋳型剤 / 低温徐凝固 / 手動式超微量駆動装置 / 保温ホルダー / 走査型電子顕微鏡
Research Abstract

How blood vessel originated in metanephros is a question discussed for a long time. Angiogenesis and vasculogenesis are two main hypotheses. The former is related to blood vessel invasion, and the latter differentiation of progenitors from mesenchymal cells. However, which one is prerequisite to the very early stage has not been clarified. We developed a method to elucidate temporal angiogenesis originated from dorsal aorta and established a method to constitute the blood vessel network by applying the angiogenesis.
Using resin combined with ink-template method to inject into umbilical cord, we succeeded in the identification of occurrence time of angiogenesis. In vivo transplantation subsequent to in vitro culture, we succeeded in generation of a full blood vessel network in kidney. These techniques are useful to both kidney and other organs.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (33 results)

All 2014 2013 2012 Other

All Presentation (30 results) (of which Invited: 1 results) Book (2 results) Remarks (1 results)

  • [Presentation] セラミックス製担体培養による発生期腎臓血管ネットワークの形成2014

    • Author(s)
      西村裕介、王碧昭
    • Organizer
      第13回日本再医療学会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] セラミックス製担体培養による発生期腎臓血管ケットワークの形成2014

    • Author(s)
      西村裕介、王碧昭
    • Organizer
      第13回日本再生医療学会
    • Place of Presentation
      京都、国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] 背側大動脈による腎血管形成の解明2013

    • Author(s)
      西村裕介、王碧昭
    • Organizer
      筑波技術研究会
    • Place of Presentation
      筑波
    • Year and Date
      2013-03-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 発生期腎臓におけるangiogenesisの観察法2013

    • Author(s)
      西村裕介、王碧昭
    • Organizer
      BMNM2013(日本顕微鏡学会バイオメディカルニューマイクロスコープ分科会
    • Place of Presentation
      東京
    • Year and Date
      2013-03-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Angiogenesis in developing kidney and transplanted kidney2013

    • Author(s)
      Yusuke Nishimura, PiChao Wang
    • Organizer
      2013 ASCB (American Society of Cell Biology) Annual Meeting
    • Place of Presentation
      New Orleans, U.S.A.
    • Related Report
      2013 Final Research Report
  • [Presentation] 拍動流培養装置を用いた発生期腎臓血管形成の検証2013

    • Author(s)
      西村裕介、王碧昭
    • Organizer
      第51回日本人工臓器学会、第5回国際人工臓器学術大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] 発生期腎血管形成の解析と血管構築の試み2013

    • Author(s)
      西村裕介、王碧昭
    • Organizer
      日本動物細胞工学会
    • Place of Presentation
      福井
    • Related Report
      2013 Final Research Report
  • [Presentation] 発生期腎血管形成の解析及び血管構築の試み2013

    • Author(s)
      西村裕介、王碧昭
    • Organizer
      日本動物細胞工学会
    • Place of Presentation
      福井、ホテルフジタ福井
    • Related Report
      2013 Annual Research Report
  • [Presentation] 拍動流培養装置を用いた発生期腎臓血管形成の検証2013

    • Author(s)
      西村裕介、王碧昭
    • Organizer
      第51会日本人工臓器学会・第5回国際人工臓器学術大会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] Angiogenesis in developing kidney and transplanted kidney2013

    • Author(s)
      Y. Nishimura, H.Hsu, PC. Wang
    • Organizer
      American Society of Cell Biology Annual Meeting
    • Place of Presentation
      Convention Center, New Orleans, Louisiana, U.S.A.
    • Related Report
      2013 Annual Research Report
  • [Presentation] Elucidation of dorsal artery invasion to metanephros2012

    • Author(s)
      Yusuke Nishimura, PiChao Wang
    • Organizer
      細胞アッセイ研究会
    • Place of Presentation
      東京
    • Year and Date
      2012-12-10
    • Related Report
      2013 Final Research Report
  • [Presentation] 発生期腎臓におけるangiogenesisの時空間的形成の解明2012

    • Author(s)
      西村裕介、王碧昭
    • Organizer
      第21回日本次世代人工腎臓研究会
    • Place of Presentation
      東京
    • Year and Date
      2012-09-29
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス胎児期においける腎血管の血管鋳型法開発2012

    • Author(s)
      西村裕介、王碧昭
    • Organizer
      筑波技術研究会
    • Place of Presentation
      つくば
    • Year and Date
      2012-03-02
    • Related Report
      2013 Final Research Report
  • [Presentation] Generation of main blood vessels in the developing kidney2012

    • Author(s)
      Yusuke Nishimura, PiChao Wang
    • Organizer
      YABEC (Young Asia of Bio-Engineering Conference)2012
    • Place of Presentation
      Tokushima, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] 発生期講じんにおける血管新生の解明2012

    • Author(s)
      西村裕介、王碧昭
    • Organizer
      第64回日本生物工学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] 時空間的形成による腎血管の解明2012

    • Author(s)
      王碧昭、西村裕介
    • Organizer
      第44回日本化学工学会
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report
  • [Presentation] Elucidation of angiogenesis of blood vessel during kidney development2012

    • Author(s)
      Yusuke Nishimura, PiChao Wang
    • Organizer
      The 5^<th> International Biotechnology Symposium and Exhibition
    • Place of Presentation
      Kaegu, Korea
    • Related Report
      2013 Final Research Report
  • [Presentation] 発生期腎血管形成におけるangiogenesisとvasculogenesisの解明2012

    • Author(s)
      西村裕介、王碧昭
    • Organizer
      ナノバイオメディカル学会
    • Place of Presentation
      つくば
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス発生期腎血管鋳型の観察2012

    • Author(s)
      西村裕介、王碧昭
    • Organizer
      第57回日本透析医学会
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス胎仔期における腎血管の血管鋳型法2012

    • Author(s)
      西村裕介、王 碧昭
    • Organizer
      第57回日本透析医学会
    • Place of Presentation
      札幌、札幌市教育文化会館
    • Related Report
      2012 Research-status Report
  • [Presentation] 発生腎血管形成におけるAngiogenesisとVasculogenesisの解明2012

    • Author(s)
      西村裕介、王 碧昭
    • Organizer
      ナノ・バイオメディカル学会
    • Place of Presentation
      つくば、産業技術総合研究所
    • Related Report
      2012 Research-status Report
  • [Presentation] Elucidation of angiogenesis of blood vessel during kidney development2012

    • Author(s)
      Yusuke Nishimura, PiChao Wang
    • Organizer
      The 15th International Biotechnology Symposium and Exhibition (IBS2012)
    • Place of Presentation
      Daegu, Korea,  Daegu Exhibition & Convention Center
    • Related Report
      2012 Research-status Report
  • [Presentation] 時空間的形成による腎血管の解明2012

    • Author(s)
      王 碧昭、西村裕介
    • Organizer
      第44回日本化学工学会
    • Place of Presentation
      仙台 東北大学工学部
    • Related Report
      2012 Research-status Report
  • [Presentation] 発生期後腎における血管新生の解明2012

    • Author(s)
      西村裕介、王 碧昭
    • Organizer
      第64回日本生物工学会
    • Place of Presentation
      神戸、国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] Generation of main blood vessels in the developing kidney2012

    • Author(s)
      Yusuke Nishimura, PiChao Wang
    • Organizer
      YABEC 2012 (Young Asian of Bio-Engineering Committee 2012)
    • Place of Presentation
      Tokushima, Tokushima University
    • Related Report
      2012 Research-status Report
  • [Presentation] 発生期腎臓におけるangiogenesisの時空間的形成の解明

    • Author(s)
      西村裕介、王 碧昭
    • Organizer
      第21回日本次世代人工腎臓研究会
    • Place of Presentation
      東京 大手町サンケイプラザ
    • Related Report
      2012 Research-status Report
  • [Presentation] The elucidation of dosal artery invasion to metanephros

    • Author(s)
      Yusuke Nishimura, PiChao Wang
    • Organizer
      細胞アッセイ研究会
    • Place of Presentation
      東京、東京大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 発生期腎臓におけるangiogenesisの観察法

    • Author(s)
      西村裕介、王 碧昭
    • Organizer
      BMNM2013(日本顕微鏡学会バイオメディカルニュ-マイイクロスコ-プ分科会)
    • Place of Presentation
      東京 帝京大学板橋キャンパス医学部
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 背側大動脈による腎血管形成の解明

    • Author(s)
      西村裕介、王 碧昭
    • Organizer
      つくば技術懇話会
    • Place of Presentation
      つくば 筑波大学
    • Related Report
      2012 Research-status Report
  • [Presentation] マウス胎児期における腎血管の血管鋳型法開発

    • Author(s)
      西村裕介 王 碧昭
    • Organizer
      筑波技術研究会
    • Place of Presentation
      つくば独法産業技術総合研究所
    • Related Report
      2011 Research-status Report
  • [Book] 動物細胞培養2014

    • Author(s)
      王碧昭、他多数
    • Total Pages
      700
    • Publisher
      技術情報協会
    • Related Report
      2013 Final Research Report
  • [Book] 動物細胞培養2014

    • Author(s)
      王碧昭 他多数(総数120名)
    • Total Pages
      700
    • Publisher
      技術情報協会
    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.agbi.tsukuba.ac.jp/~cogitate/index.html

    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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