| Project/Area Number |
23580131
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KANO Kenji 京都大学, 大学院農学研究科, 教授 (10152828)
HIGUCHI Yoshiki 兵庫県立大学, 大学院生命理学研究科, 教 授 (90183574)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | バイオ燃料電池 / マルチ銅オキシダーゼ / ラッカーゼ / 酵素電極 / 機能改変 |
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| Research Abstract |
For the development of the platinum-substitute anodic enzyme catalysts for bio-fuel cell, I performed the in silico screening to find new multi-copper oxidases and the mutations against the common enzymes to improve their oxygen reduction activity. In the screening for the new multi-copper oxidases, I found two laccase genes from an anaerobic bacterium and an insect. The heterologous expression systems for the laccase genes were successfully constructed, and the recombinant laccase from anaerobe was found to have unreported properties so far. Furthermore, the improvements of the bacterial common enzymes in the oxygen-reduction activity and the electrode response were achieved by protein engineering.
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