| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
A novel strategy preparing raw materials for pharmaceuticals such as shikimic acid for Tamiflu was established applying koji-mold catalyst to yerba mate tea. Chlorogenic acid (CGA) hydrolase (CGase) was potently induced in mycelia such as Aspergillus niger when grown on yerba mate extract. Heat treatment of mycelia at 40˚C for 30 min destroyed polyphenol oxidase but CGase was highly resistant and survived during the heat treatment.Thus, the heated mycelia were found useful as a kind of immobilized catalyst of CGase. Some model reaction using immobilized heated mycelia as CGase was studied. This is the most outstanding point in this study and this method can be accepted for industrial production of QA and caffeic acid (CA). QA can be converted to shikimate by two enzyme systems of acetic acid bacteria, as has already been established in the previous study. Simultaneous isolation of QA, CGA, and CA was established by a simple ion exchange column chromatography.
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