| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Research Abstract |
In order to search for novel human N-myristoylated proteins that can be applied for biomarkers, the susceptibility of human cDNA clones in human cDNA resources to protein N-myristoylation was evaluated by metabolic labeling of proteins expressed using an insect cell-free protein synthesis system. For this analysis, 178 cDNA clones with an N-terminal Met-Gly motif were selected as potential candidates from ~6,300 Kazusa ORFeome project (KOP) human cDNA clones. The susceptibility of these cDNA clones to protein N-myristoylation was evaluated by metabolic labeling experiments both in an insect cell-free protein synthesis system and in transfected mammalian cells. As a result, the products of 35 out of ~6,300 cDNA clones were found to be novel human N-myristoylated proteins. These novel N-myristoylated proteins contain not only physiologically important proteins but also many disease-associated proteins that can be applied for biomarkers.
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