| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Carbohydrate-recognition mechanisms of calcium-dependent lectins, which play important roles in molecular recognition systems in organisms, were analyzed, and a novel artificial lectin with a designed binding-specificity was produced. In addition, site-directed mutational and three-dimensional structural analyses of the hemolytic lectin CEL-III revealed that it forms heptamers in target cell membrane with extensive conformational changes, forming the transmembrane pores that lead to hemolysis or cell death. These results may provide valuable information on various pore-forming toxin proteins widely distributed in the nature.
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