Carbohydrate-recognition and transmembrane ion-channel-formation mechanisms of calcium-dependent lectins
Project/Area Number |
23580137
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied biochemistry
|
Research Institution | Nagasaki University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
UNNO Hideaki 長崎大学, 大学院工学研究科, 助教 (10452872)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | レクチン / 糖鎖 / 部位特異的変異 / X線結晶構造解析 / 蛋白質工学 / デンドリマー / 部位特異的変異体 / カルシウム結合蛋白質 |
Research Abstract |
Carbohydrate-recognition mechanisms of calcium-dependent lectins, which play important roles in molecular recognition systems in organisms, were analyzed, and a novel artificial lectin with a designed binding-specificity was produced. In addition, site-directed mutational and three-dimensional structural analyses of the hemolytic lectin CEL-III revealed that it forms heptamers in target cell membrane with extensive conformational changes, forming the transmembrane pores that lead to hemolysis or cell death. These results may provide valuable information on various pore-forming toxin proteins widely distributed in the nature.
|
Report
(4 results)
Research Products
(72 results)