| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
In this research, a feedback regulatory mechanism of auxin biosynthesis has been studied by transcription analysis of auxin biosynthetic genes and metabolic analysis in auxin excess and deficient condition of Arabidopsis seedlings. Auxin excess and deficient conditions were produced by feeding of synthetic auxin and auxin biosynthesis inhibitors to wild type Arabidopsis and by using genetic mutants in auxin biosynthesis. As a result, auxin biosynthesis has been demonstrated to be regulated at the step of YUCCA, a rate-limiting step of auxin biosynthesis, through the negative feedback manner. This suggests that auxin biosynthesis as well as conjugation and degradation contributes to auxin homeostasis. We consider that our results may be useful for agricultural technology such as promotion of rooting and stable fruiting.
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