The analysis of auxin biosynthetic pathway using metabolic intermediates
Project/Area Number |
23580144
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied biochemistry
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Research Institution | Yokohama City University |
Principal Investigator |
SUZUKI Masashi 横浜市立大学, 付置研究所, その他 (30342801)
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Co-Investigator(Renkei-kenkyūsha) |
SHIMADA Yukihisa 横浜市立大学, 木原生物学研究所, 教授 (30300875)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | インドールピルビン酸 / オーキシン / 生合成 / 阻害剤 / フィードバック / YUCCA / シロイヌナズナ / 遺伝子発現 / 代謝中間体 / TAA |
Research Abstract |
In this research, a feedback regulatory mechanism of auxin biosynthesis has been studied by transcription analysis of auxin biosynthetic genes and metabolic analysis in auxin excess and deficient condition of Arabidopsis seedlings. Auxin excess and deficient conditions were produced by feeding of synthetic auxin and auxin biosynthesis inhibitors to wild type Arabidopsis and by using genetic mutants in auxin biosynthesis. As a result, auxin biosynthesis has been demonstrated to be regulated at the step of YUCCA, a rate-limiting step of auxin biosynthesis, through the negative feedback manner. This suggests that auxin biosynthesis as well as conjugation and degradation contributes to auxin homeostasis. We consider that our results may be useful for agricultural technology such as promotion of rooting and stable fruiting.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Analysis of a putative auxin biosynthesis inhibitor, indole-3-oxoethylphophonic acid, in Arabidopsis2014
Author(s)
Ishida Y., Hayashi K., Soeno K., Asami T., Nakamura S., Suzuki M., Nakamura A. and Shimada Y.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 78
Issue: 1
Pages: 67-70
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] オーキシンのシグナルを抑制するシロイヌナズナDof型転写因子の解析2013
Author(s)
石田遥介, 中村郁子, 鈴木優志, 筧雄介, 橋本恵, 近藤陽一, 松井南, 豊岡公徳, 林謙一郎, 浅見忠男, 嶋田幸久
Organizer
第54回日本植物生理学会年会
Place of Presentation
岡山市
Related Report
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[Presentation] Characterization of a Dof-type transcription factor, which plays a negative role in auxin signaling in Arabidopsis root elongation2012
Author(s)
Ishida Y, Nakamura A, Suzuki M, Kakei Y, Kondou Y, Matsui M, Toyooka K, Hayashi K, Asami T, Shimada Y.
Organizer
AUXIN2012
Place of Presentation
Hawaii, USA
Related Report
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[Presentation] Characterization of a Dof-type transcription factor, which plays a negative role in auxin signaling in Arabidopsis root elongation2012
Author(s)
Yosuke Ishida, Ayako Nakamura, Masashi Suzuki, Yusuke Kakei, Youichi Kondou, Minami Matsui, Kiminori Toyooka, Kenichiro Hayashi, Tadao Asami and Yukihisa Shimada
Organizer
AUXIN 2012
Place of Presentation
Hawaii, USA
Related Report
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