Functional analysis of novel terpene cyclases that resemble transporter

Research Project

Project/Area Number	23580153
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Bioproduction chemistry/Bioorganic chemistry
Research Institution	Meiji University
Principal Investigator	KUSHIRO Tetsuo 明治大学, 農学部, 准教授 (80373299)
Project Period (FY)	2011 – 2013
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000) Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	メロテルペノイド / 糸状菌 / テルペン環化酵素 / ポリケタイド / 生合成
Research Abstract	Pyripyropene, produced by a fungus Aspergillus fumigatus, is a meroterpenoid that is expected to be developed as a cholesterol lowering drug. Among the pyripyropene biosynthetic gene cluster, a novel terpene cyclase was identified that resembled more like a transporter. In this study, detailed analysis of its active site residue was carried out. Besides, a second meroterpenoid biosynthetic gene cluster was identified from A. fumigatus as well as from a fungus Fusarium graminearum, which causes Fusarium head blight. The PKS among the second gene cluster in A. fumigatus was shown to produce TAL.