Application of a new HPLC-ESR system for water soluble components of foods and vegetables
Project/Area Number |
23580166
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Food science
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Research Institution | Kyoto Institute of Technology |
Principal Investigator |
TAJIMA Kunihiko 京都工芸繊維大学, 工芸科学研究科, 教授 (50163457)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
|
Keywords | スーパーオキシドラジカル / 抗酸化活性 / 青果物水溶性成分 / HPLC-ESR分析法 / スーパーオキソドラジカル / 青果物 / 抗酸化物質 / HPLC-ESR装置 / 食品成分 / HPLC / ESR |
Research Abstract |
Recently, flow-injection ESR systems for the quantitative spin-trapping ESR measurements have been developed to estimate the second order rate constants (ks) of the reaction between hydroxyl radical and scavengers. The flow-ESR technique is employed for development of a new HPLC-ESR system for the post-column screening of superoxide radical scavengers. This system was composed of a flow UV-irradiation cell for photo-excitation of riboflavin (Rf) to generate superoxide radical in the presence of an appropriate electron donor such as EDTA,. The HPLC- ESR analysis was performed for water soluble components derived from 20 vegetables, such as onion, spinach, cabbage, root radish, etc.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Ability of Water-Soluble Biosubstances to Eliminate Hydroxyl and Superoxide Radicals Examined by Spin-Trapping ESR Measurements: Two-Dimensional Presentation of Antioxidative Ability2013
Author(s)
Nakajima, A., Masuda, Y., Matsuda, F., Tajima, K., Sameshima, H., Ikenoue, T.
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Journal Title
Bioscience Biotechnology and Bochemistry,
Volume: 77
Pages: 324-331
NAID
Related Report
Peer Reviewed
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[Journal Article] Abilities of Antioxidants to Eliminate the Peroxyl Radical Derived from 2,2'-Azobis(2-Amidinopropane) Dihydrochloride (AAPH)2013
Author(s)
Nakajima, A., Masuda, Y., Matsuda, F., Tajima, K., Sameshima, H., Ikenoue, T.
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Journal Title
Applied Magnetic Resonance,
Volume: 44
Issue: 9
Pages: 997-1005
DOI
Related Report
Peer Reviewed
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[Journal Article] Evaluation of the Antioxidant Abilities of Water-Soluble Biosubstances Using the ORAC-ESR Assay in Combination with the Hydroxyl and Superoxide Radical-Eliminating Abilities2013
Author(s)
Nakajima, A., Masuda, Y., Matsuda, F., Tajima, K., Sameshima, H., Ikenoue, T.
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Journal Title
Applied Magnetic Resonance,
Volume: 44
Issue: 12
Pages: 1419-1430
DOI
Related Report
Peer Reviewed
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[Journal Article] Application of Flow-ESR Techniques for Mechanistic Study of Biological Radical Reactions : HPLC-ESR Spin-Trapping System for Post-Column Evaluation of the Superoxide Radical Scavenging Activity of Column Eluates2011
Author(s)
Kawai, K., Sakurai, Y., Komatsu. R., Morimoto, C., Nakajima, A., Kanaori, K., Tajima, K.
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Journal Title
Applied Magnetic Resonance
Volume: Vol. 40, No. 4
Pages: 449-458
Related Report
Peer Reviewed
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[Book] 抗酸化食品研究2011
Author(s)
田嶋邦彦、川井清史、加藤寛之、櫻井康博、金折賢二
Total Pages
197
Publisher
光琳
Related Report