Prevention of chronic inflammation targeting angiopoietin-like protein 2

• Project/Area Number: 23580174
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: University of Miyazaki
• Principal Investigator: NISHIYAMA Kazuo 宮崎大学, 農学部, 准教授 (40164610)
• Co-Investigator(Renkei-kenkyūsha): YAMASAKI Masao 宮崎大学, 農学部, 准教授 (80381060) ; SAKAKIBARA Yoichi 宮崎大学, 農学部, 教授 (90295197)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 慢性炎症 / アンジオポエチン様因子２ / β1インテグリン / α-リポ酸 / 脂肪細胞 / リポ酸 / インスリン / β1-インテグリン / アンジオポエチン様タンパク質２ / 膀胱がん細胞 / インテグリンβ1 / アンジオポイエチン様因子２ / がん / がん細胞

Research Abstract

Recently chronic inflammation is regarded as causes of various diseases, such as diabetes, atherosclerosis and cancer. Angiopoietin-like protein 2 (ANGPTL2), a novel pro-inflammatory adipokine, has been reported to have important roles in the chronic inflammation and pathogenesis of various diseases. ANGPTL2 induces inflammatory signals via its receptor, integrin. In this study, we investigated the effect of alpha-lipoic acid (LA) on the expression of beta1-integrin in a human bladder cancer cell line and ANGPTL2 expression in 3T3-L1 murine adipocyte cell line. LA inhibited both beta1-integrin and ANGPTL2 expressions in a dose dependent manner, suggesting that LA might reduce chronic inflammation by inhibiting ANGPTL2 signaling pathway.

Research Products

• [Journal Article] Identification of a novel flavonoid glycoside sulfotransferase in Arabidopsis thaliana. (2014)
  • Author(s): Hashiguchi, T., Sakakibara, Y, Shimohira, T., Kurogi, K, Yamasaki, M., Nishiyama, K., Akashi, R., Liu, M. ・C., Suiko, M.
  • Journal Title: The Journal of Biochemistry Volume: 155 Issue: 2 Pages: 91-97
  • DOI: 10.1093/jb/mvt102
  • Peer Reviewed / Open Access
• [Journal Article] Carnosol, rosemary ingredient, induces apoptosis in adult T-cell leukemia/lymphoma cells via glutathione depletion; proteomic approach using fluorescent two-dimensional differential gel electrophoresis (2014)
  • Author(s): Yo-ichi Ishida, Masao Yamasaki, Chizuko Yukizaki, Kazuo Nishiyama, Hirohito Tsubouchi, Akihiko Okayama, Hiroaki Kataoka
  • Journal Title: Human Cell Volume: 27(2) Issue: 2 Pages: 68-77
  • DOI: 10.1007/s13577-013-0083-6
  • Peer Reviewed
• [Journal Article] α-Lipoic acid suppresses migration and invasion via downregulation of cell surface β1-integrin expression in bladder cancer cells (2014)
  • Author(s): Masao Yamasaki, Masahiro Iwase, Kazuo Kawano, Yoichi Sakakibara, Masahito Suiko, Masahiro Ikeda, Kazuo Nishiyama
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 54 Issue: 1 Pages: 18-25
  • DOI: 10.3164/jcbn.13-57
  • NAID: 130004466739
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Induction of Apoptotic Cell Death in HL-60 Cells by Jacaranda Seed Oil Derived Fatty Acids (2013)
  • Author(s): Masao Yamasaki, Chihiro Motonaga, Marino Yokoyama, Aya Ikezaki, Tomoka Kakihara, Rintaro Hayasegawa, Kaede Yamasaki, Masanobu Sakono, Yoichi Sakakibara, Masahito Suiko, Kazuo Nishiyama
  • Journal Title: Journal of Oleo Science Volume: 62 Issue: 11 Pages: 925-932
  • DOI: 10.5650/jos.62.925
  • NAID: 130004436004
  • ISSN: 1345-8957, 1347-3352
  • Peer Reviewed
• [Journal Article] Genistein induces apoptotic cell death associated with inhibition of the NF-B pathway in adult T-cell leukemia cells (2013)
  • Author(s): Masao Yamasaki, Yoshihiro Mine, Misato Nishimura, Satoshi Fujita, Yoichi Sakakibara, Masahito Suiko, Kazuhiro Morishita and Kazuo Nishiyama
  • Journal Title: Cell Biology International Volume: 37 Issue: 7 Pages: 742-747
  • DOI: 10.1002/cbin.10101
  • Peer Reviewed
• [Journal Article] Jacaric Acid is Rapidly Metabolized to Conjugated Linoleic Acid in Rats (2013)
  • Author(s): Ryo Kijima, Taro Honma, Junya Ito, Masao Yamasaki, Aya Ikezaki, Chihiro Motonaga, Kazuo Nishiyama, and Tsuyoshi Tsuduki
  • Journal Title: Journal of Oleo Science Volume: 62 Issue: 5 Pages: 305-312
  • DOI: 10.5650/jos.62.305
  • NAID: 130004436045
  • ISSN: 1345-8957, 1347-3352
  • Peer Reviewed
• [Journal Article] Antioxidant Activities and Anti-Cancer Cell Proliferation Properties of Natsuhaze (Vaccinium oldhamii Miq.), Shashanbo (V. bracteatum Thunb.) and Blueberry Cultivars (2013)
  • Author(s): Hirotoshi Tsuda, Hisato Kunitake, Ryoko Kawasaki-Takaki, Kazuo Nishiyama, Masao Yamasaki, Haruki Komatsu and Chizuko Yukizaki
  • Journal Title: Plants Volume: 2(1) Issue: 1 Pages: 57-71
  • DOI: 10.3390/plants2010057
  • Peer Reviewed
• [Journal Article] Conjugated Linoleic Acids Inhibit Hypoxia Inducible Factor-1α Stabilization under Hypoxic Condition in Human Hepatocellular Carcinoma Cells (2012)
  • Author(s): Yamasaki M , Nagatomo T, Matsuyama T, Ikeho Y, Kato E, Nishiyama K, Sakakibara Y, Suiko M, Nishiyama K.
  • Journal Title: Journal of Oleo Science Volume: 61 Issue: 9 Pages: 491-496
  • DOI: 10.5650/jos.61.491
  • NAID: 130004435980
  • ISSN: 1345-8957, 1347-3352
  • Peer Reviewed
• [Journal Article] Jacaric Acid, a Linolenic Acid Isomer with a Conjugated Triene System, Reduces Stearoyl-CoA Desaturase Expression in Liver of Mice (2012)
  • Author(s): Shinohara, N., Ito, J.,Tsuzuki, T., Honma, T., Kijima, R., Sugawara, S., Arai, T., Yamasaki, M., Ikezaki, A., Yokoyama, M. Nakagawa, K., Miyazawa, T., Ikeda, I
  • Journal Title: Journal of Oleo Science Volume: 61 Issue: 8 Pages: 433-441
  • DOI: 10.5650/jos.61.433
  • NAID: 130004435972
  • ISSN: 1345-8957, 1347-3352
  • Peer Reviewed
• [Journal Article] Selective Inhibition by Apocynin of the Proliferation and Adhesion to Fibronectin of v-H-<i>ras</i>-Transformed 3Y1 Cells (2012)
  • Author(s): Yamasaki M, Iwase M, Kawano K, Sakakibara Y, Suiko M, Nishiyama K.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 6 Pages: 1177-1181
  • DOI: 10.1271/bbb.120061
  • NAID: 10030818269
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Jacaric acid, a linolenic acid isomer with a conjugated triene system, has a strong antitumor effect in vitro and in vivo (2012)
  • Author(s): Shinohara, N., Tsuduki T., Ito, J., Honma, T., Kijima, R., Sugawara, S., Arai, T., Yamasaki, M., Ikezaki, A., Yokoyama, M., Nishiyama, K., Nakagawa, K., Miyazawa, T., Ikeda, I.
  • Journal Title: Biochim. Biophys. Acta Volume: 1821 Issue: 7 Pages: 980
  • DOI: 10.1016/j.bbalip.2012.04.001
  • Peer Reviewed
• [Journal Article] Effect of Highly Lipolyzed Goat Cheese on HL-60 Human Leukemia Cells : Antiproliferative Activity and Induction of Apoptotic DNA Damage (2012)
  • Author(s): Yasuda, S., Kuwata, H., Kawamoto, K., Shirakawa, J., Atobe, S., Hoshi, Y., Yamasaki, M., Nishiyama, K., Tachibana,H., Yamada, K., Kobayashi,H., Igoshi, K.
  • Journal Title: J Dairy Sci Volume: 95(5) Pages: 2248-2260
  • Peer Reviewed
• [Journal Article] Alpha lipoic acid selectively inhibits proliferation and adhesion to fibronectin of v-H-ras-transformed 3Y1 cells (2012)
  • Author(s): Yamasaki M, Iwase M, Kawano K, Sakakibara Y, Suiko M, Nishiyama K.
  • Journal Title: J Clin Biochem Nutr Volume: 50(3) Pages: 234-240
  • NAID: 130004879270
  • Peer Reviewed
• [Journal Article] Effect of Highly Lipolyzed Goat Cheese on HL-60 Human Leukemia Cells:Antiproliferative Activity and Induction of Apoptotic DNA Damage. (2012)
  • Author(s): Tachibana, H. et al.
  • Journal Title: J. Dairy Sci. Volume: 95 Issue: 5 Pages: 2248-2260
  • DOI: 10.3168/jds.2011-4153
  • Peer Reviewed
• [Journal Article] Selective inhibition by apocynin of the proliferation and adhesion to fibronectin of v-H-ras-transformed 3Y1 cells. (2012)
  • Author(s): Yamasaki M, Iwase M, Kawano K, Sakakibara Y, Suiko M, Nishiyama K.
  • Journal Title: Biosci Biotechnol Biochem Volume: 76 Pages: 1177-1181
  • NAID: 10030818269
  • Peer Reviewed
• [Journal Article] jacaric acid, a linolenic acid isomer with a conjugated triene system, reduces stearoyl-CoA desaturase expression in liver of mice. (2012)
  • Author(s): Shinohara N, Ito J, Tsuduki T, Honma T, Kijima R, Sugawara S, Arai T, Yamasaki M, Ikezaki A, Yokoyama M, Nishiyama K, Nakagawa K, Miyazawa T, Ikeda I
  • Journal Title: J Oleo Sci. Volume: 61 Pages: 433-441
  • NAID: 130004435972
  • Peer Reviewed
• [Journal Article] Conjugated linoleic acids inhibit hypoxia inducible factor-1α stabilization under hypoxic condition in human hepatocellular carcinoma cells. (2012)
  • Author(s): Yamasaki M, Nagatomo T, Matsuyama T, Ikeho Y, Kato E, Nishiyama K, Sakakibara Y, Suiko M, Nishiyama K.
  • Journal Title: J Oleo Sci. Volume: 61 Pages: 491-506
  • NAID: 130004435980
  • Peer Reviewed
• [Journal Article] Efficient Approach for Simultaneous Estimation of the Multiple Health-Promoting Effects of Foods (2011)
  • Author(s): Nagahama K, Eto N, Yamamori K, Nishiyama K, Sakakibara Y, Iwata T, Uchida A, Yoshihara I, Suiko M.
  • Journal Title: J Agric Food Chem Volume: 59(16) Issue: 16 Pages: 8575-8588
  • DOI: 10.1021/jf201836g
  • Peer Reviewed
• [Journal Article] Enzymatic Sulfation of Tocopherols and Tocopherol Metabolites by Human Cytosolic Sulfotransferases (2011)
  • Author(s): Hashiguchi, T, et.al.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 10 Pages: 1951-1956
  • DOI: 10.1271/bbb.110352
  • NAID: 10029872846
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Enzymatic Sulfation of Tocopherols and Tocopherol Metabolites by Human Cytosolic Sulfotransferases (2011)
  • Author(s): Hashiguchi T, Kurogi K, Sakakibara Y, Yamasaki M, Nishiyama K, Yasuda S, Liu MC, Suiko M
  • Journal Title: Biosci Biotechnol Biochem Volume: 75 Pages: 1951-1956
  • NAID: 10029872846
  • Peer Reviewed
• [Journal Article] An Efficient Approach for Simultaneous Estimation of the Multiple Health-Promoting Effects of Foods (2011)
  • Author(s): Nagahama K, Eto N, Yamamori K, Nishiyama K, Sakakibara Y, Iwata T, Uchida A, Yoshihara I, Suiko M.
  • Journal Title: J Agric Food Chem Volume: 59 Pages: 8575-8588
  • Peer Reviewed
• [Journal Article] サンプル選別と拡張重み更新型SOMを統合したたんぱく質発現量からの食品機能性推定法 (2011)
  • Author(s): 山森一人、吉原郁夫、江藤望、西山和夫
  • Journal Title: 情報処理学会論文誌数理モデル化と応用 Volume: 4 Pages: 69-80
  • NAID: 40019257199
  • Peer Reviewed
• [Journal Article] The importance of 1,2-dithiolane structure in alpha-lipoic acid for the downregulation of cell surfaceβ1-integrin expression of human bladder cancer cells
  • Author(s): Masao Yamasaki, Shozen Soda, Masahiro Iwase, Yoichi Sakakibara, Masahito Suiko, Kazuo Nishiyama
  • Journal Title: Biosci Biotechnol Biochem Volume: (in press)
  • Peer Reviewed
• [Presentation] 脂肪組織を標的としたγ-リノレン酸の抗炎症作用 (2013)
  • Author(s): 中村崇弘、山崎正夫、西山和夫
  • Organizer: 平成25年度日本食品科学工学会西日本支部および日本栄養・食糧学会九州・沖縄支部合同大会
  • Place of Presentation: 福岡
  • Year and Date: 2013-10-19
• [Presentation] ブルーベリー葉抽出物が脂肪細胞の機能に及ぼす影響 (2013)
  • Author(s): 松山勇介、甲斐孝憲、亀長浩蔵、松浦靖、柚木崎千鶴子、西山和夫、山崎正夫
  • Organizer: 平成25年度日本食品科学工学会西日本支部および日本栄養・食糧学会九州・沖縄支部合同大会
  • Place of Presentation: 福岡
  • Year and Date: 2013-10-19
• [Presentation] CordycepinによるLPS誘導性NO産生抑制効果 (2013)
  • Author(s): 今村健太、浅井桃子、菅本和寛、松本朋子、山崎有美、亀井一郎、服部貴博、岸本正興、新坂誠司、西山和夫、山崎正夫
  • Organizer: 第481回日本農芸化学会関西・中四国・西日本支部合同大会
  • Place of Presentation: 広島
  • Year and Date: 2013-09-06
• [Presentation] 細胞接着分子integrinを標的としたα-リポ酸のガン細胞の浸潤・転移抑制 (2013)
  • Author(s): 早田昌然、岩瀬将弘、榊原陽一、水光正仁、山崎正夫、西山和夫
  • Organizer: 2013年度生物機能研究会
  • Place of Presentation: 熊本
  • Year and Date: 2013-07-20
• [Presentation] α-リポ酸によるアンジオポエチン様タンパク質2の産生抑制作用 (2013)
  • Author(s): 久米田翔子、山崎正夫、西山和夫
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台
  • Year and Date: 2013-03-25
• [Presentation] ブルーベリー葉抽出物が脂肪細胞の機能に及ぼす影響 (2013)
  • Author(s): 松山勇介、甲斐孝憲、亀長浩蔵、竹下正彦、菊池幸治、松浦靖、柚木崎千鶴子、西山和夫、山崎正夫
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台
  • Year and Date: 2013-03-25
• [Presentation] 細胞接着分子integrinを標的としたα-リポ酸のガン細胞の浸潤・転移抑制 (2013)
  • Author(s): 早田昌然、岩瀬将弘、榊原陽一、水光正仁、山崎正夫、西山和夫
  • Organizer: 生物機能研究会
  • Place of Presentation: 熊本市
• [Presentation] CordycepinによるLPS誘導性NO産生抑制効果 (2013)
  • Author(s): 今村健太、浅井桃子、菅本和寛、松本朋子、山崎有美、亀井一郎、服部貴博、岸本正興、新坂誠司、西山和夫、山崎正夫
  • Organizer: 第481回日本農芸化学会関西・中四国・西日本支部合同大会
  • Place of Presentation: 広島市
• [Presentation] ブルーベリー葉抽出物が脂肪細胞の機能に及ぼす影響 (2013)
  • Author(s): 松山勇介、甲斐孝憲、亀長浩蔵、松浦靖、柚木崎千鶴子、西山和夫、山崎正夫
  • Organizer: 平成25年度日本食品科学工学会西日本支部および日本栄養・食糧学会九州・沖縄支部合同大会
  • Place of Presentation: 福岡市
• [Presentation] 脂肪組織を標的としたγ-リノレン酸の抗炎症作用 (2013)
  • Author(s): 中村崇弘、山崎正夫、西山和夫
  • Organizer: 平成25年度日本食品科学工学会西日本支部および日本栄養・食糧学会九州・沖縄支部合同大会
  • Place of Presentation: 福岡市
• [Presentation] α-リポ酸のβ1-integrinを標的とした膀胱ガン細胞の浸潤、転移抑制作用 (2012)
  • Author(s): 岩瀬将弘、河野和生、山崎正夫、西山和夫
  • Organizer: 第7回αリポ酸研究会
  • Place of Presentation: 京都
  • Year and Date: 2012-10-19
• [Presentation] 冬虫夏草(Crdyceps militaris)の抗炎症作用 (2012)
  • Author(s): 今村健太、浅井桃子、菅本和寛、松本明子、山崎有美、亀井一郎、服部貴博、岸本正興、新坂誠司、西山和夫、山崎正夫
  • Organizer: 平成24年度日本農芸化学会西日本支部および日本栄養・食糧学会九州・沖縄支部合同大会
  • Place of Presentation: 鹿児島
  • Year and Date: 2012-09-29
• [Presentation] アラキドン酸、γ-リノレン酸による抗炎症作用と作用機序に関する研究 (2012)
  • Author(s): 中村崇弘、山崎正夫、西山和夫
  • Organizer: 平成24年度日本農芸化学会西日本支部および日本栄養・食糧学会九州・沖縄支部合同大会
  • Place of Presentation: 鹿児島
  • Year and Date: 2012-09-29
• [Presentation] α-リポ酸の細胞接着因子integrinを標的とした膀胱ガン転移抑制機構 (2012)
  • Author(s): 岩瀬将弘、河野和生、山崎正夫、西山和夫
  • Organizer: 平成24年度日本農芸化学会西日本支部および日本栄養・食糧学会九州・沖縄支部合同大会
  • Place of Presentation: 鹿児島
  • Year and Date: 2012-09-29
• [Presentation] 脂肪酸による一酸化窒素及びMCP-1産生抑制作用 (2012)
  • Author(s): 中村崇弘、山崎正夫、西山和夫
  • Organizer: 第66回日本栄養・食糧学会大会
  • Place of Presentation: 仙台
  • Year and Date: 2012-05-19
• [Presentation] ブルーベリー葉抽出物による脂肪細胞の機能制御と炎症予防 (2011)
  • Author(s): 中村崇弘、梁井綾香、平原秀秋、中原徳昭、山崎正夫、西山和夫
  • Organizer: フードサイエンスフォーラム第20回記念学術集会
  • Place of Presentation: 宮崎
  • Year and Date: 2011-09-17
• [Presentation] ブルーベリー葉抽出物による脂肪細胞の機能制御と炎症予防 (2011)
  • Author(s): 中村崇弘、梁井綾香、平原秀秋、中原徳昭、山崎正夫、西山和夫
  • Organizer: 平成23年度日本栄養・食糧学会九州・沖縄支部および日本食品科学工学会西日本支部合同大会
  • Place of Presentation: 佐賀
  • Year and Date: 2011-09-04
• [Presentation] α-リポ酸によるv-Ha-ras形質転換細胞のβ1-integrinの発現抑制 (2011)
  • Author(s): 岩瀬将弘、河野和生、山崎正夫、水光正仁、榊原陽一、西山和夫
  • Organizer: 2011年度生物機能研究会
  • Place of Presentation: 福岡
  • Year and Date: 2011-07-02
• [Presentation] α-リポ酸のアポトーシス誘導機構解明 (2011)
  • Author(s): 久米田翔子、有薗裕規、西本健太郎、北岡翔太、川邉暁子、山崎正夫、榊原陽一、水光正仁、西山和夫
  • Organizer: 第65回日本栄養・食糧学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-14
• [Presentation] 肝ガン細胞における共役リノール酸の致死作用
  • Author(s): 野見山将太、山崎正夫、岡本威明、須田泰司、榊原陽一、水光正仁、西山和夫
  • Organizer: 平成23年度日本農芸化学会西日本支部・中四国支部合同大会
  • Place of Presentation: 宮崎
• [Presentation] Genistein inhibits the growth of human T-cell leukemia virus type 1-infected cell line Hut102
  • Author(s): Y. Mine, M. Nishimura, Y. Sakakibara, M. Suiko, K. Morishita, M. Yamasaki, and K. Nishiyama
  • Organizer: 2011 International Conference on Food Factors
  • Place of Presentation: Taipei
• [Presentation] 低酸素下における共役リノール酸の抗ガン作用機構の解明
  • Author(s): 長友琢、松山哲也、池保有理、加藤恵美子、西山慧、山崎正夫、西山和夫
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
• [Book] 生物機能実験法 (2012)
  • Author(s): 西山和夫、岩瀬将弘、山崎正夫
  • Total Pages: 13
  • Publisher: がん悪性形質のレドックス制御機能評価法
• [Book] 食と健康を支援する 第2集 (2012)
  • Author(s): 永濱清子、江藤望、山森一人、西山和夫、榊原陽一、吉原郁夫、水光正仁
  • Total Pages: 18
  • Publisher: 生物機能研究所