| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Although anthocyanin glycosides are major forms distributed in many plant foods and promised as chemopreventive source, molecular mechanisms remain unclear. This study aims to identify the molecular targets of bioactive anthocyanins using chemical biology methodologies. The results showed that bioactive anthocyanins had defined potential of affinity and binding to MAP kinases in the order of MEK > ERK > B-Raf without ATP-competitive manner. Therefore, bioactive anthocyanins suppressed the activation of these MAP kinase to prevent cell transformation and inflammation in mouse paw edema induced by carcinogens. On the other hand, the specific and inhibitory effects were weaker than that by the specific inhibitors of MAP kinases. These findings provide the molecular evidence for understanding the chemopreventive effects of bioactive anthocyanins.
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