Novel anti-infection Drug-delivery strategy by using ovotransferrin and its domains

• Project/Area Number: 23580177
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Kagoshima University
• Principal Investigator: IBRAHIM HISHAM RADWAN 鹿児島大学, 農学部, 教授 (90274836)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 食品生化学 / Drug-delivery / Anti-infection / Transferrin receptor / Ovotransferrin / Antimicrobial / anti-infection / Transferrin-receptor / Drug Delivery / Transferrin Receptor / Iron-binding / Pathogens / Protein Delivery / Anti-infective Drug

Research Abstract

The results demonstrate that ovotransferrin (OTf) binds different insoluble sulfa antibiotics and for complex in which these hydrophobic antibiotics become totally water soluble. The antibiotics-off complexes showed potent antimicrobial activity against several pathogens or either Gram-positive or Gram-negative strains. Strikingly, the complexes were able to reach and kill pathogens intracellularly infecting human colon cells (HCT-116), and the mechanism was found to be mediated through endocytosis via transferring receptor found at the surface of human cells. The finding herald a fascinating opportunity with potential application of OTf as drug carrier for the treatment of infectious diseases.

Research Products

• [Journal Article] Ovotransferrin as novel antibiotic-targeting molecule with potent antimicrobial action (2014)
  • Author(s): Ibrahim H.R.; Tatsumoto S.; Ono H.; Raspoet R.; Van Immerseel F.
  • Journal Title: Intl. J. Mol. Sci Volume: (in press)
  • Peer Reviewed
• [Journal Article] Ovotransferrin as novel antibiotic-targeting molecule with potent antimicrobial action (2014)
  • Author(s): Ibrahim H.R.; Tatsumoto S.; Ono H.; Raspoet R.; Van Immerseel F.
  • Journal Title: International Journal of Molecular Science Volume: in press
  • Peer Reviewed
• [Journal Article] Expression of ovotransferrin enhances tolerance of yeast cells toward oxidative stress (2013)
  • Author(s): Ibrahim H.R.; Hozono A.; Fukami M.; Shaban M.A.; Miyata T.
  • Journal Title: J. Agri. Food Chem Volume: 61 (26)
  • Peer Reviewed
• [Journal Article] Expression of ovotransferrin enhances tolerance of yeast cells toward oxidative stress (2013)
  • Author(s): Ibrahim H.R.; Hozono A.; Fukami M.; Shaban M.A.; Miyata T.
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 61 (26) Issue: 26 Pages: 6358-6365
  • DOI: 10.1021/jf401152e
  • Peer Reviewed
• [Journal Article] Expression of ovotransferrin enhances tolerance of yeast cells toward oxidative stress (2013)
  • Author(s): (3) Ibrahim H.R.; Hozono A.; Fukami M.
  • Journal Title: Journal of Agricultural Food Chemistry Volume: In Press
  • Peer Reviewed
• [Journal Article] Ovotransferrin as novel antibiotic-targeting molecule with potent antimicrobial action (2013)
  • Author(s): (4) Ibrahim H.R.; Ono H.; Tatsumoto S.; Raspoet R.; Van Immerseel F.
  • Journal Title: International Journal Molecular Science Volume: In Press
  • Peer Reviewed
• [Journal Article] Anti-infective drugs : why should we pay attention? (2012)
  • Author(s): Ibrahim, H.R.
  • Journal Title: Outlook on Developing Drugs. Open Access Pages: 1-2
• [Journal Article] Effect of polymorphism in egg white lysozyme on muramidase and antibacterial activities as well as hatchability in the Japanese quail (Coturnix japonica) (2012)
  • Author(s): Myint, S.L., Kinoshita, K., Shimogiri, T., Ibrahim, H.R., Tsusaki, T., Tanoue, T., Kawabe, K., Maeda, Y. and Okamoto, S.
  • Journal Title: J. Anim. Sci. (JAS) Volume: 90 (6) Pages: 1747-1755
  • Peer Reviewed
• [Journal Article] Anti-infective drugs: why should we pay attention? (2012)
  • Author(s): (1) Ibrahim H.R.
  • Journal Title: Developing Drugs Volume: Open Access Pages: 1-2
  • Peer Reviewed
• [Journal Article] Effect of polymorphism in egg white lysozyme on muramidase and antibacterial activities as well as hatchability in the Japanese quail (Coturnix japonica) (2012)
  • Author(s): (2) Myint S.L.; Kinoshita K.; Shimogiri T.; Ibrahim H.R.; Tsusaki T.; Tanoue T.; Kawabe K.; Maeda Y.; Okamoto S.
  • Journal Title: Journal of Animal Science Volume: 90 (6) Issue: 6 Pages: 1747-1755
  • DOI: 10.2527/jas.2011-4501
  • Peer Reviewed
• [Journal Article] Human lysozyme possesses novel antimicrobial peptides within its N-terminal domain that target bacterial respiration (2011)
  • Author(s): Ibrahim, H.R., Imazato, K. and Ono, H.
  • Journal Title: J. Agri. Food Chem Volume: 59 (18) Pages: 10336-10345
  • Peer Reviewed
• [Journal Article] Potent antimicrobial action of triclosan-lysozyme complex against skin pathogens mediated through drug-targeted delivery mechanism (2011)
  • Author(s): Hoq, M.I. and Ibrahim, H.R.
  • Journal Title: Eur. J. Pharm. Sci Volume: 42 Pages: 130-137
  • Peer Reviewed
• [Journal Article] Potent antimicrobial action of triclosan-lysozyme complex against skin pathogens mediated through drug-targeted delivery mechanism (2011)
  • Author(s): Hoq, M.I. and Ibrahim, H.R.
  • Journal Title: European Journal of Pharmaceutical Science Volume: 42 Issue: 1-2 Pages: 130-137
  • DOI: 10.1016/j.ejps.2010.11.002
  • Peer Reviewed
• [Journal Article] Human lysozyme possesses novel antimicrobial peptides within its N-terminal domain that target bacterial respiration (2011)
  • Author(s): Ibrahim, H.R.; Imazato, K. and Ono, H.
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 59 (18) Issue: 18 Pages: 10336-10345
  • DOI: 10.1021/jf2020396
  • Peer Reviewed
• [Presentation] ecoding the Functional Roles of Polypeptides in Egg Albumen : New Therapeutic Hope (2013)
  • Author(s): Ibrahim, H.R.
  • Organizer: in the"International Workshop on MDR efflux pumps and Salmonella Infection"
  • Place of Presentation: Ghent, Belgium
  • Invited
• [Presentation] Novel Therapeutic Potential of Egg Ovotransferrin (2013)
  • Author(s): Ibrahim, H.R.
  • Organizer: in the 104^<th> AOCS Conference, Montreal
  • Place of Presentation: Quebec, Canada
  • Invited
• [Presentation] Novel Antibiotics Drug-delivery Strategy by Utilizing Lysozyme as Targeting Molecule (2013)
  • Author(s): Ibrahim, H.R.; Ono, H.; Kubo, T.
  • Organizer: in the 5^<th> International Symposium on Antimicrobial Resistance in Animals and the Environment (ARAE 2013)
  • Place of Presentation: Ghent, Belgium
  • Invited
• [Presentation] Unique Antimicrobial Peptides from Lysozyme with Potent Anti-inflammatory Activities (2013)
  • Author(s): Hamasaki, K. and Ibrahim, H.R.
  • Organizer: in the The 5^<th> International Symposium on Antimicrobial Resistance in Animals and the Environment (ARAE 2013)
  • Place of Presentation: Aula, Ghent(Poster)
• [Presentation] Novel Bioptides from Lysozyme with Therapeutic Potential for Infectious and Inflammatory Diseases (2013)
  • Author(s): Ibrahim, H.R.
  • Organizer: in the 14^<th> International Conference of Functional Foods and Bioactive Compounds in the Management of Chronic Inflammation
  • Place of Presentation: University of California Los Angeles (UCLA), Los Angeles, USA)(Oral)
• [Presentation] Novel Therapeutic Potential of Egg Ovotransferrin (2013)
  • Author(s): Ibrahim, H.R.
  • Organizer: 104th AOCS Conference
  • Place of Presentation: Palace de Congres de Montreal, Canada
  • Invited
• [Presentation] Novel Antibiotics Drug-delivery Strategy by Utilizing Lysozyme as Targeting Molecule (2013)
  • Author(s): Ibrahim, H.R.
  • Organizer: 5th International Symposium on Antimicrobial Resistance in Animals and the Environment (ARAE 2013)
  • Place of Presentation: AULA, Ghent, Belgium
  • Invited
• [Presentation] Novel Bioptides from Lysozyme with Therapeutic Potential for Infectious and Inflammatory Diseases (2013)
  • Author(s): Ibrahim, H.R.
  • Organizer: 14th International Conference of Functional Foods and Bioactive Compounds in the Management of Chronic Inflammation
  • Place of Presentation: UCLA, Los Angeles, USA
  • Invited
• [Presentation] Decoding the Functional Roles of Polypeptides in Egg Albumen: New Therapeutic Hope (2013)
  • Author(s): Ibrahim H.R.
  • Organizer: Workshop on multi drug resistance (MDR) efflux pumps and Salmonella
  • Place of Presentation: Ghent, Belgium
  • Invited
• [Presentation] Potential Therapeutic Bio-peptides from Beneath the Shell of Egg (2012)
  • Author(s): Ibrahim, H.R.
  • Organizer: in the 3^<rd> BanffEgg 2012 Forum"Innovations in Egg Utilization Research and Development
  • Place of Presentation: Banff, Alberta, Canada
  • Invited
• [Presentation] Therapeutic Potential of Novel Bioactive Peptides from Honey (2012)
  • Author(s): Ibrahim, H.R.
  • Organizer: in the 103^<rd> AOCS Conference
  • Place of Presentation: Long Beach, California, USA
  • Invited
• [Presentation] 感染症と戦う卵白由来の機能性ペプチド (2012)
  • Author(s): イブラヒムヒッシャム
  • Organizer: "機能性食品用ペプチド"研究会
  • Place of Presentation: 大阪国際会議場、大阪
  • Invited
• [Presentation] Therapeutic Potential of Novel Bioactive Peptides from Honey (2012)
  • Author(s): Ibrahim H.R.
  • Organizer: 103rd AOCS Annual Meeting
  • Place of Presentation: Long Beach, Los Angeles, USA
  • Invited
• [Presentation] Potential Therapeutic Biopeptides from Beneath the Shell of Egg (2012)
  • Author(s): Ibrahim, H.R.
  • Organizer: Banff Egg Forum 2012(招待講演)
  • Place of Presentation: Banff, Alberta, Canada
• [Presentation] Ovotransferrin and its Peptides Confer in vivo Resistance to Oxidative Stress (2011)
  • Author(s): Ibrahim, H.R.
  • Organizer: in the 102^<nd> AOCS Conference- "Health Aspects of Food Proteins and Peptides" Symposium
  • Place of Presentation: Cincinnati, Ohio, USA
  • Invited
• [Presentation] 治療薬として卵白ペプチドの新しいポテンシャル (2011)
  • Author(s): イブラヒムヒッシャム
  • Organizer: "食品の流通システムと食品に関する最新の科学"研究会
  • Place of Presentation: 日本大学、藤沢、日本
  • Invited
• [Presentation] 治療薬として卵白ペプチドの新しいポテンシャル (2011)
  • Author(s): イブラヒム ヒッシャム
  • Organizer: 食品の流通システム・食品加工実習所シンポジウム(招待講演)
  • Place of Presentation: 日本大学-日本
• [Remarks]
  • URL: http://chem.agri.kagoshima-u.ac.jp/~hishamri/