| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
GlcN may have a potential not only to induce the osteoblastic cell differentiation especially at middle-late stages, but also to suppress the osteoclastic cell differentiation, thereby possibly increasing bone matrix deposition and decreasing bone resorption, and eventually modulate bone metabolism in OA.
Next, we searched the target genes in chondrocyte cell line by incubating in the presence of GlcN. RT-PCR analyses revealed that GlcN significantly increased the sirtuin (SIRT) 1 mRNA expression levels. Furthermore, western blot analyses revealed that GlcN significantly increased the protein level of SIRT1. Moreover, GlcN did not essentially affect the SIRT1 expression levels in osteoblastic cells. GlcN is able to upregulate the mRNA and protein levels of SIRT1 in chondrocytes, thereby possibly exhibiting the protective action on OA.
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