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Construction of trichothecene detection system and screening system for detoxifying microorganisms

Research Project

Project/Area Number 23580185
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionToyo University

Principal Investigator

ANDO Naoko  東洋大学, 理工学部, 准教授 (70360485)

Co-Investigator(Kenkyū-buntansha) MINEGISHI Hiroaki  東洋大学, バイオ・ナノエレクトロニクス研究センター, 研究助手 (30440019)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsカビ毒 / トリコテセン / 検出系 / 解毒分解 / スクリーニング / 食の安全 / 高感度検出系の構築 / 解毒微生物 / マイコトキシン / 解毒・分解
Research Abstract

Trichothecenes are mycotoxins which are produced by Fusarium and other genera. They contaminate important crops, and can cause serious health problems in humans and livestock. In this research, we found 22 trichothecene resistance genes in Saccharomyces cerevisiae. We constructed multiple gene deletion mutants, and among them, the triple mutant which deleted pdr5, erg6, and rpb4, showed the highest sensitivity toward various trichothecenes. We constructed an effective detective system using disc diffusion test, which allows us to detect deoxynivalenol contaminated with the provisional level in wheat and wheat flower. Next, we screened for microoganisms which can detoxify T-2 toxin, deoxynivalenol and 4-acetylnivalenol. We collected ~10,000 microorganisms mainly from soil, and we found that there were more than 10% microorganisms can deacetylate T-2 toxin and 4-acetyl nivalenol, which resulted in reducing toxicity. However, no organisms can detoxify deoxynivalenol.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (30 results)

All 2014 2013 2012 Other

All Journal Article (7 results) (of which Peer Reviewed: 5 results) Presentation (23 results)

  • [Journal Article] Development of a highly sensitive yeast bioassay for trichothecene detection2013

    • Author(s)
      TANAKA, A., YAMANE, Y., KOMIYA, Y., YAMAUCHI, K., SUGIYAMA, T., ECHIGO, A., USAMI, R., YOSHIDA, Y, ABE, F., MINEGISI, H. and TAKAHASHI-ANDO, N.
    • Journal Title

      JSM Mycotoxins

      Volume: 63 Issue: 2 Pages: 161-170

    • DOI

      10.2520/myco.63.161

    • NAID

      130003384017

    • ISSN
      0285-1466, 1881-0128
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 天然毒素耐性を担う酵母遺伝子の探索と応用利用(2)2013

    • Author(s)
      安藤直子
    • Journal Title

      工業技術

      Volume: 第35号

    • Related Report
      2013 Final Research Report
  • [Journal Article] Functional screening for resistance genes against trichothecenes in the library of <i>Saccharomyces cerevisiae</i> deletion mutants2013

    • Author(s)
      Naoko TAKAHASHI-ANDO, Akira TANAKA, Yohsuke SEKIMOTO, Kohta YAMAUCHI, Akinobu ECHIGO, Ron USAMI, Fumiyoshi ABE, Hiroaki MINEGISHI
    • Journal Title

      JSM Mycotoxins

      Volume: 63 Issue: 1 Pages: 9-15

    • DOI

      10.2520/myco.63.9

    • NAID

      10031161942

    • ISSN
      0285-1466, 1881-0128
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional screening for resistance genes against trichothecenes in the library of Saccharomyces cerevisiae deletion mutants.2013

    • Author(s)
      1. TAKAHASHI-ANDO, N., TANAKA, A., SEKIMOTO, Y., YAMAUCHI, K., ECHIGO, A., USAMI, R., ABE, F. and MINEGISHI, H.
    • Journal Title

      Mycotoxins

      Volume: 63 Pages: 9-15

    • NAID

      10031161942

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Production of 3-acetylnivalenol by transgenic <i>Fusarium graminearum</i> expressing <i>Tri13</i> of type A trichothecene-producer: participation of the encoded cytochrome P450 monooxygenase in type B trichothecene biosynthesis2012

    • Author(s)
      Kazuyuki MAEDA, Takeshi TOKAI, Hinayo ICHIKAWA, Naoko TAKAHASHI-ANDO, Nobuo OGURA, Katsuyoshi YONEYAMA, Minoru YOSHIDA, Makoto KIMURA
    • Journal Title

      JSM Mycotoxins

      Volume: 62 Issue: 2 Pages: 83-90

    • DOI

      10.2520/myco.62.83

    • NAID

      10031026572

    • ISSN
      0285-1466, 1881-0128
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization ofβ-cyclodextrin glucanotransferase from Bacillus sp. strain 149-12012

    • Author(s)
      Ryoichi KUMAZAKI, Hiroyuki MINEGISHI, Akinobu ECHIGO, Yasuhiro SHIMANE, Naoko TAKAHASHI-ANDO, Ron USAMI
    • Journal Title

      J. Jpn Soc. Extromophiles

      Volume: 11 Pages: 26-32

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 天然毒素耐性を担う酵母遺伝子の探索と応用利用2012

    • Author(s)
      安藤直子
    • Journal Title

      工業技術

      Volume: 第35号 Pages: 32-37

    • Related Report
      2013 Final Research Report
  • [Presentation] 新規非天然型トリコテセンの同定と毒性評価2014

    • Author(s)
      鎌田賢太朗他
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県)
    • Year and Date
      2014-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] トリコテセンC-4位アセチル化酵素TRI7の発現と性状解析2014

    • Author(s)
      田中彰他
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県)
    • Year and Date
      2014-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 新規非天然型トリコテセンの同定と毒性評価2014

    • Author(s)
      鎌田賢太朗、田中彰、多久島遼、相川俊一、吉田泰彦、安藤直子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      明治大学生田キャンパス (神奈川県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] トリコテセンC-4位アセチル化酵素TRI7の発現と性状解析2014

    • Author(s)
      田中彰、杉山智樹、吉田泰彦、安藤直子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      明治大学生田キャンパス (神奈川県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 放線菌由来抗がん性物質Collismycin Aの作用標的同定2014

    • Author(s)
      井上堯、川谷誠、室井誠、二村友史、青野晴美、浦本昌和、本郷やよい、越野広雪、五十嵐康弘、安藤直子、長田裕之
    • Organizer
      日本農芸化学会
    • Place of Presentation
      明治大学生田キャンパス (神奈川県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 多重遺伝子破壊酵母によるトリコテセン簡易検出系の構築2013

    • Author(s)
      田中彰他
    • Organizer
      日本マイコトキシン学会第73回学術講演会
    • Place of Presentation
      大阪府立大学(大阪)
    • Year and Date
      2013-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] トリコテセン自己耐性遺伝子Tri101のHEK293細胞への導入とC-3位アセチル化体の毒性評価2013

    • Author(s)
      多久島遼他
    • Organizer
      日本マイコトキシン学会第73回学術講演会
    • Place of Presentation
      大阪府立大学(大阪)
    • Year and Date
      2013-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 異なるFusarium属菌の遺伝子破壊株とフィーディングを用いた非天然型トリコテセンの創成2013

    • Author(s)
      鎌田賢太朗他
    • Organizer
      日本マイコトキシン学会第73回学術講演会
    • Place of Presentation
      大阪府立大学(大阪)
    • Year and Date
      2013-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] トリコテセンC-4位アセチル化酵素TRI7の性状解析2013

    • Author(s)
      杉山智樹他
    • Organizer
      日本マイコトキシン学会第73回学術講演会
    • Place of Presentation
      大阪府立大学(大阪)
    • Year and Date
      2013-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 出芽酵母の遺伝子多重破壊株によるトリコテセン検出系の構築2013

    • Author(s)
      杉山智樹他
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2013-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of a target for collismycin A, a cytotoxic microbial product, by proteomic profiling.2013

    • Author(s)
      KAWATANI, M., INOUE, G., MUROI, M., FUTAMURA, Y., AONO, H., HONGO, Y., KOSHINO, H., IGARASHI, Y, TAKAHASHI-ANDO, N., OSADA, H.
    • Organizer
      AACR Molecular Targets and Cancer Therapeutics
    • Place of Presentation
      Hynes Convention Center (Boston)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 酵母の遺伝子多重破壊株によるトリコテセン検出系の構築2013

    • Author(s)
      杉山智樹、山根由美、田中彰、小宮洋平、越後輝敦、宇佐美論、峯岸宏明、安藤直子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      仙台 東北大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 出芽酵母の遺伝子多重破壊株によるトリコテセン感受性の解析2012

    • Author(s)
      山根由美他
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学(京都)
    • Year and Date
      2012-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Screening for resistant genes against trichothecenes and magnetic particles in the library of Saccharomyces cerevisiae deletion mutants2012

    • Author(s)
      Yohei KOMIYA,et al.
    • Organizer
      BNERC and IJAA Joint International Symposium on Advanced Science and Nanotechnology
    • Place of Presentation
      Toyo University, Hakusan campus (Tokyo)
    • Related Report
      2013 Final Research Report
  • [Presentation] Construction of screening system for T-2 toxin detoxifying microorganisms and characterization of crude detoxifying enzymes2012

    • Author(s)
      Masatoshi KOBAYASHI, et al.
    • Organizer
      BNERC and IJAA Joint International Symposium on Advanced Science and Nanotechnology
    • Place of Presentation
      Toyo University, Hakusan campus (Tokyo)
    • Related Report
      2013 Final Research Report
  • [Presentation] Screening for resistant genes against trichothecenes and magnetic particles in the library of Saccharomyces cerevisiae deletion mutants.2012

    • Author(s)
      KOMIYA, Y., TANAKA, A., YAMANE Y., SYGIYAMA, T., ECHIGO, A., USAMI, R., MINEGHISHI H. and TAKAHASHI-ANDO, N.
    • Organizer
      BNERC and IJAA Joint International Symposium on Advanced Science and Nanotechnology.
    • Place of Presentation
      Tokyo, Toyo University
    • Related Report
      2012 Research-status Report
  • [Presentation] Construction of screening system for T-2 toxin detoxifying microorganisms and characterization of crude detoxifying enzymes.2012

    • Author(s)
      KOBAYASHI, M., TANAKA, A., ECHIGO, A., USAMI, R., MINEGHISHI H. and TAKAHASHI-ANDO, N.
    • Organizer
      BNERC and IJAA Joint International Symposium on Advanced Science and Nanotechnology.
    • Place of Presentation
      Tokyo, Toyo University
    • Related Report
      2012 Research-status Report
  • [Presentation] 出芽酵母の遺伝子多重破壊株によるトリコテセン感受性の解析2012

    • Author(s)
      山根 由美、山内 皓太、関本 陽介、渡井 智裕、峯岸 宏明、宇佐美 論、安藤 直子
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学(京都市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 放線菌由来抗がん性物質Collismycin Aの作用機構解析

    • Author(s)
      井上尭、川谷誠、室井誠、二村友史、青野晴美、浦本昌和、本郷やよい、越野広雪、五十嵐康弘、安藤直子、長田裕之
    • Organizer
      化学生物学研究会
    • Place of Presentation
      北里大学(東京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 多重遺伝子破壊酵母によるトリコテセン簡易検出系の構築

    • Author(s)
      田中彰、杉山智樹、鎌田賢太朗、多久島遼、宇佐美論、吉田泰彦、越後輝敦、峯岸宏明、安藤直子
    • Organizer
      日本マイコトキシン学会第73回学術講演会
    • Place of Presentation
      大阪府立大学(大阪府)
    • Related Report
      2013 Annual Research Report
  • [Presentation] トリコテセン自己耐性遺伝子Tri101のHEK293細胞への導入とC-3位アセチル化体の毒性評価

    • Author(s)
      多久島遼、田中彰、鎌田賢太朗、安藤直子
    • Organizer
      日本マイコトキシン学会第73回学術講演会
    • Place of Presentation
      大阪府立大学(大阪府)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 異なるFusarium属菌の遺伝子破壊株とフィーディングを用いた非天然型トリコテセンの創成

    • Author(s)
      鎌田賢太朗、田中彰、杉山智樹、多久島遼、安藤直子
    • Organizer
      日本マイコトキシン学会第73回学術講演会
    • Place of Presentation
      大阪府立大学(大阪府)
    • Related Report
      2013 Annual Research Report
  • [Presentation] トリコテセンC-4位アセチル化酵素TRI7の性状解析

    • Author(s)
      杉山智樹、田中彰、安藤直子
    • Organizer
      日本マイコトキシン学会第73回学術講演会
    • Place of Presentation
      大阪府立大学(大阪府)
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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