Construction of trichothecene detection system and screening system for detoxifying microorganisms
| Project/Area Number |
23580185
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Toyo University |
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Principal Investigator |
ANDO Naoko 東洋大学, 理工学部, 准教授 (70360485)
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| Co-Investigator(Kenkyū-buntansha) |
MINEGISHI Hiroaki 東洋大学, バイオ・ナノエレクトロニクス研究センター, 研究助手 (30440019)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | カビ毒 / トリコテセン / 検出系 / 解毒分解 / スクリーニング / 食の安全 / 高感度検出系の構築 / 解毒微生物 / マイコトキシン / 解毒・分解 |
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| Research Abstract |
Trichothecenes are mycotoxins which are produced by Fusarium and other genera. They contaminate important crops, and can cause serious health problems in humans and livestock. In this research, we found 22 trichothecene resistance genes in Saccharomyces cerevisiae. We constructed multiple gene deletion mutants, and among them, the triple mutant which deleted pdr5, erg6, and rpb4, showed the highest sensitivity toward various trichothecenes. We constructed an effective detective system using disc diffusion test, which allows us to detect deoxynivalenol contaminated with the provisional level in wheat and wheat flower. Next, we screened for microoganisms which can detoxify T-2 toxin, deoxynivalenol and 4-acetylnivalenol. We collected ~10,000 microorganisms mainly from soil, and we found that there were more than 10% microorganisms can deacetylate T-2 toxin and 4-acetyl nivalenol, which resulted in reducing toxicity. However, no organisms can detoxify deoxynivalenol.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Development of a highly sensitive yeast bioassay for trichothecene detection2013
Author(s)
TANAKA, A., YAMANE, Y., KOMIYA, Y., YAMAUCHI, K., SUGIYAMA, T., ECHIGO, A., USAMI, R., YOSHIDA, Y, ABE, F., MINEGISI, H. and TAKAHASHI-ANDO, N.
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Journal Title
JSM Mycotoxins
Volume: 63
Issue: 2
Pages: 161-170
DOI
NAID
ISSN
0285-1466, 1881-0128
Related Report
Peer Reviewed
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[Journal Article] Functional screening for resistance genes against trichothecenes in the library of Saccharomyces cerevisiae deletion mutants.2013
Author(s)
1. TAKAHASHI-ANDO, N., TANAKA, A., SEKIMOTO, Y., YAMAUCHI, K., ECHIGO, A., USAMI, R., ABE, F. and MINEGISHI, H.
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Journal Title
Mycotoxins
Volume: 63
Pages: 9-15
NAID
Related Report
Peer Reviewed
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[Presentation] Identification of a target for collismycin A, a cytotoxic microbial product, by proteomic profiling.2013
Author(s)
KAWATANI, M., INOUE, G., MUROI, M., FUTAMURA, Y., AONO, H., HONGO, Y., KOSHINO, H., IGARASHI, Y, TAKAHASHI-ANDO, N., OSADA, H.
Organizer
AACR Molecular Targets and Cancer Therapeutics
Place of Presentation
Hynes Convention Center (Boston)
Related Report
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[Presentation] Screening for resistant genes against trichothecenes and magnetic particles in the library of Saccharomyces cerevisiae deletion mutants.2012
Author(s)
KOMIYA, Y., TANAKA, A., YAMANE Y., SYGIYAMA, T., ECHIGO, A., USAMI, R., MINEGHISHI H. and TAKAHASHI-ANDO, N.
Organizer
BNERC and IJAA Joint International Symposium on Advanced Science and Nanotechnology.
Place of Presentation
Tokyo, Toyo University
Related Report
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[Presentation] Construction of screening system for T-2 toxin detoxifying microorganisms and characterization of crude detoxifying enzymes.2012
Author(s)
KOBAYASHI, M., TANAKA, A., ECHIGO, A., USAMI, R., MINEGHISHI H. and TAKAHASHI-ANDO, N.
Organizer
BNERC and IJAA Joint International Symposium on Advanced Science and Nanotechnology.
Place of Presentation
Tokyo, Toyo University
Related Report
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