Anti-diabetes and its mechanism using food factors via incretin action.

• Project/Area Number: 23580187
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Chubu University
• Principal Investigator: TSUDA Takanori 中部大学, 応用生物学部, 准教授 (90281568)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 食品成分 / 糖尿病 / 食品 / 食品因子

Research Abstract

Increase endogenous GLP-1 secretion from intestinal L cells by food factors is one of the crucial targets for prevention and treatment of diabetes. In the present study, we demonstrate that curcumin, a yellow pigment, significantly increases GLP-1 secretion in L cells model, and we clarified the structure activity relationship using curcumin derivatives. Also, concerning the secretory mechanism, the significant increase in GLP-1 secretion by curcumin involved the CaMKII pathway, and was independent of extracellular signal-regulated kinase, PKC, and the cAMP/PKA-related pathway.

Research Products

• [Journal Article] Stimulation of glucose uptake by theasinensins through the AMP-activated protein kinase pathway in rat skeletal muscle cells (2014)
  • Author(s): Ju Qiu, J., Maekawa, K., Kitamura, Y., Miyata, Y., Tanaka, T., Tanaka , T., Soga, M., Tsuda, T., Matsui, T.
  • Journal Title: Biochem. Pharmacol Volume: 87 Pages: 344-351
  • Peer Reviewed
• [Journal Article] Stimulation of glucose uptake by theasinensins through the AMP-activated protein kinase pathway in rat skeletal muscle cells. (2014)
  • Author(s): Ju Qiu, J., Maekawa, K., Kitamura, Y., Miyata, Y., Tanaka, T., Tanaka , T., Soga, M., Tsuda, T., Matsui, T.
  • Journal Title: Biochem. Pharmacol. Volume: 87 Issue: 2 Pages: 344-351
  • DOI: 10.1016/j.bcp.2013.10.029
  • Peer Reviewed
• [Journal Article] Curcumin stimulates glucagon-like peptide-1 secretion in GLUTag cells via Ca^<2+>/calmodulin-dependent kinase II activation (2013)
  • Author(s): Takikawa, M., Kurimoto, Y., Tsuda, T.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 435 Pages: 165-70
  • Peer Reviewed
• [Journal Article] Black soybean seed coat extract ameliorates hyperglycemia and insulin sensitivity via the activation of AMP-activated protein kinase in diabetic mice (2013)
  • Author(s): Kurimoto, Y., Shibayama, Y., Inoue, S., Soga, M., Takikawa, M., Ito, C., Nanba, F., Yoshida, T., Yamashita, Y., Ashida, H., Tsuda, T.
  • Journal Title: J. Agric. Food Chem Volume: 61 Pages: 5558-64
  • Peer Reviewed
• [Journal Article] 10-hydroxy-2-decenoic acid, a unique medium-chain fatty acid, activates AMP-activated protein kinase in L6 myotubes and mice (2013)
  • Author(s): Takikawa, M., Kumagai, A., Hirata, H., Soga, M., Yamashita, Y., Ueda, M., Ashida, H., Tsuda, T.
  • Journal Title: Mol. Nutr. Food. Res Volume: 57 Pages: 1794-802
  • Peer Reviewed
• [Journal Article] Curcumin stimulates glucagon-like peptide-1 secretion in GLUTag cells via Ca2+/calmodulin-dependent kinase II activation. (2013)
  • Author(s): Takikawa, M., Kurimoto, Y., Tsuda, T.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 435 Issue: 2 Pages: 165-170
  • DOI: 10.1016/j.bbrc.2013.04.092
  • Peer Reviewed
• [Journal Article] Black soybean seed coat extract ameliorates hyperglycemia and insulin sensitivity via the activation of AMP-activated protein kinase in diabetic mice. (2013)
  • Author(s): Kurimoto, Y., Shibayama, Y., Inoue, S., Soga, M., Takikawa, M., Ito, C., Nanba, F., Yoshida, T., Yamashita, Y., Ashida, H., Tsuda, T.
  • Journal Title: J. Agric. Food Chem. Volume: 61 Issue: 23 Pages: 5558-5564
  • DOI: 10.1021/jf401190y
  • Peer Reviewed
• [Journal Article] 10-hydroxy-2-decenoic acid, a unique medium-chain fatty acid, activates AMP-activated protein kinase in L6 myotubes and mice. (2013)
  • Author(s): Takikawa, M., Kumagai, A., Hirata, H., Soga, M., Yamashita, Y., Ueda, M., Ashida, H., Tsuda, T.
  • Journal Title: Mol. Nutr. Food. Res. Volume: 57 Issue: 10 Pages: 1794-1802
  • DOI: 10.1002/mnfr.201300041
  • Peer Reviewed
• [Journal Article] Dietary anthocyanin-rich plants: Biochemical basis and recent progress in health benefits studies (2012)
  • Author(s): Tsuda, T.
  • Journal Title: Mol. Nutr. Food Res Volume: 56 (Invited review) Pages: 159-70
  • Peer Reviewed
• [Journal Article] Anthocyanins as Functional Food Factors - Chemistry, Nutrition and Health Promotion - (2012)
  • Author(s): Tsuda, T.
  • Journal Title: Food Sci. Technol. Res Volume: 18 (Invited review) Pages: 315-24
  • NAID: 10030922596
  • Peer Reviewed
• [Journal Article] Genistein and daidzein, typical soy isoflavones, inhibit TNF-α-mediated downregulation of adiponectin expression via different mechanisms in 3T3-L1 adipocytes (2012)
  • Author(s): Yanagisawa, M., Sugiya, M., Iijima, H., Nakagome, I., Hirono, S., Tsuda, T.
  • Journal Title: Mol. Nutr. Food Res Volume: 56 Pages: 1783-93
  • Peer Reviewed
• [Journal Article] Anthocyanins as Functional Food Factors<br>&#8212; Chemistry, Nutrition and Health Promotion &#8212; (2012)
  • Author(s): Tsuda, T.
  • Journal Title: Food Science and Technology Research Volume: 18 Issue: 3 Pages: 315-324
  • DOI: 10.3136/fstr.18.315
  • NAID: 130001922858
  • ISSN: 1344-6606, 1881-3984
  • Peer Reviewed
• [Journal Article] Genistein and daidzein, typical soy isoflavones, inhibit TNF-alpha-mediated downregulation of adiponectin expression via different mechanisms in 3T3-L1 adipocytes. (2012)
  • Author(s): Yanagisawa, M., Sugiya, M., Iijima, H., Nakagome, I., Hirono, S., Tsuda, T.
  • Journal Title: Mol. Nutr. Food Res. Volume: 56 Issue: 12 Pages: 1783-1793
  • DOI: 10.1002/mnfr.201200284
  • Peer Reviewed
• [Journal Article] Dietary anthocyanin-rich plants: Biochemical basis and recent progress in health benefits studies. (2012)
  • Author(s): Tsuda, T.
  • Journal Title: Mol. Nutr. Food Res. Volume: 56 Issue: 1 Pages: 159-170
  • DOI: 10.1002/mnfr.201100526
  • Peer Reviewed
• [Journal Article] Brazilian propolis-derived components inhibit TNF-α-mediated downregulation of adiponectin expression via different mechanisms in 3T3-L1 adipocytes (2011)
  • Author(s): Ikeda, R, Yanagisawa, M., Takahashi, N., Kawada, T., Kumazawa, S., Yamaotsu, N., Nakagome, I., Shuichi Hirono, S., Tsuda, T.
  • Journal Title: Biochim. Biophys. Acta Volume: 1810 Pages: 695-703
  • Peer Reviewed
• [Journal Article] A black soybean seed coat extract prevents obesity and glucose intolerance by up-regulating uncoupling proteins and down-regulating inflammatory cytokines in high-fat diet-fed mice (2011)
  • Author(s): Kanamoto, Y., Yamashita, Y., Namba, F., Yoshida, T., Tsuda, T., Fukuda, I., Nakamura-Tsuruta, S., and Ashida, H.
  • Journal Title: J. Agric. Food Chem Volume: 59 Pages: 8985-93
  • Peer Reviewed
• [Journal Article] Oral toxicological studies of black soybean (Glycine max) hull extract:Acute studies in rats and mice, and chronic studies in mice. 2011.11 (2011)
  • Author(s): Fukuda, I., Tsutsui, M., Yoshida, T., Toda, T., Tsuda, T., Ashida, H.
  • Journal Title: Food Chem. Toxicol Volume: 49 Pages: 3272-78
  • Peer Reviewed
• [Journal Article] Brazilian propolis-derived components inhibit TNF- a -mediated downregulation of adiponectin expression via different mechanisms in 3T3-L1 adipocytes (2011)
  • Author(s): Ikeda R、Yanagisawa M、Takahashi N、Kawada T 、 Kumazawa S 、 Yamaotsu N 、Nakagome I 、 Hirono S 、 Tsuda T
  • Journal Title: Biochimica et Biophysica Acta - General Subjects Volume: 1810(7) Issue: 7 Pages: 695-703
  • DOI: 10.1016/j.bbagen.2011.04.007
  • Peer Reviewed
• [Journal Article] A black soybean seed coat extract prevents obesity and glucose intolerance by up-regulating uncoupling proteins and down-regulating inflammatory cytokines in high-fat diet-fed mice. (2011)
  • Author(s): Kanamoto, Y., Yamashita, Y., Namba, F., Yoshida, T., Tsuda, T., Fukuda, I., Nakamura-Tsuruta, S., and Ashida, H.
  • Journal Title: J. Agric. Food Chem. Volume: 59 Issue: 16 Pages: 8985-8993
  • DOI: 10.1021/jf201471p
  • Peer Reviewed
• [Journal Article] Oral toxicological studies of black soybean (Glycine max) hull extract:Acute studies in rats and mice, and chronic studies in mice. (2011)
  • Author(s): Fukuda, I., Tsutsui, M., Yoshida, T., Toda, T., Tsuda, T., Ashida, H.
  • Journal Title: Food Chem. Toxicol. Volume: 49 Issue: 12 Pages: 3272-3278
  • DOI: 10.1016/j.fct.2011.09.022
  • Peer Reviewed
• [Presentation] 血管弛緩性ペプチドTrp-His(WH)のAMP キナーゼ活性化作用とそのメカニズム (2014)
  • Author(s): 曽我実、大橋彩加、谷口恵実、滝川雅仁、松井利郎、津田孝範
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学
• [Presentation] GLP-1 の分泌を促す食品因子の解明とその機序 (2014)
  • Author(s): 加藤正樹、谷翼、津田孝範
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学
• [Presentation] 血管弛緩性ペプチドTrp-His（WH）のAMPキナーゼ活性化作用とそのメカニズム (2014)
  • Author(s): 曽我 実、大橋 彩加、谷口 恵実、滝川 雅仁、松井 利郎、津田 孝範
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学
• [Presentation] GLP-1の分泌を促す食品因子の解明とその機序 (2014)
  • Author(s): 加藤正樹、谷翼、津田 孝範
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学
• [Presentation] 血管弛緩性ペプチドTrp-His(WH)によるAMP キナーゼ活性化作用とその機序 (2013)
  • Author(s): 曽我 実、大橋彩加、谷口恵実、滝川雅仁、松井利郎、津田孝範
  • Organizer: 第67回日本栄養・食糧学会大会
  • Place of Presentation: 名古屋大学
• [Presentation] GLP-1の分泌を促進する食品因子の検討とそのメカニズム解明 (2013)
  • Author(s): 滝川雅仁、栗本雄太、津田孝範
  • Organizer: 第67回日本栄養・食糧学会大会
  • Place of Presentation: 名古屋大学
• [Presentation] 黒大豆成分の摂取による血糖値上昇抑制作用とその作用機序解明 (2013)
  • Author(s): 栗本 雄太、柴山 勇樹、井上聖哉、曽我実、滝川 雅仁、伊藤千秋、難波文男、吉田正、山下陽子、芦田均、津田孝範
  • Organizer: 日本食品科学工学会第60回大会
  • Place of Presentation: 実践女子大学
• [Presentation] Black soybean seed coat extract ameliorates hyperglycemia and insulin sensitivity via the activation of AMP-activated protein kinase in diabetic mice (2013)
  • Author(s): Tsuda, T. et al.
  • Organizer: 7th International workshop on Anthocyanins
  • Place of Presentation: Porto 大学, Portugal
• [Presentation] Prevention of diabetes using food factors via activation of AMPK and GLP-1 secretion (2013)
  • Author(s): Tsuda, T.
  • Organizer: Annual Meeting of the International Society for Nutraceuticals and Functional Foods -Functional Foods, Nutraceuticals, Natural Health Products and Dietary Supplements-
  • Place of Presentation: Taipei International Convention Center, Taiwan
• [Presentation] GLP-1 の分泌を促進する食品因子の検討とその機序解明 (2013)
  • Author(s): 滝川 雅仁、栗本 雄太、津田 孝範
  • Organizer: 日本農芸化学会2013 年度大会
  • Place of Presentation: 東北大学
• [Presentation] 血管弛緩性ペプチドTrp-His（WH）によるAMPキナーゼ活性化作用とその機序 (2013)
  • Author(s): 曽我 実、大橋 彩加、谷口 恵実、滝川 雅仁、松井 利郎、津田 孝範
  • Organizer: 第67回日本栄養・食糧学会大会
  • Place of Presentation: 名古屋大学
• [Presentation] Black soybean seed coat extract ameliorates hyperglycemia and insulin sensitivity via the activation of AMP-activated protein kinase in diabetic mice. (2013)
  • Author(s): Kurimoto, K., Shibayama, Y., Inoue, S., Soga, M., Takikawa, M., Ito, I., Nanba, F., Yoshida, T., Yamashita, Y., Ashida, H., Tsuda, T.
  • Organizer: 7th International workshop on Anthocyanins
  • Place of Presentation: Porto, Portgal
• [Presentation] Prevention of diabetes using food factors via activation of AMPK and GLP-1 secretion. (2013)
  • Author(s): Tsuda, T.
  • Organizer: 2013 Annual Meeting of the International Society for Nutraceuticals and Functional Foods
  • Place of Presentation: Taipei,Taiwan
• [Presentation] GLP-1の分泌を促進する食品因子の検討とその機序解明 (2013)
  • Author(s): 滝川 雅仁、栗本 雄太、津田 孝範
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台
• [Presentation] ローヤルゼリー由来中鎖脂肪酸のAMP キナーゼ活性化作用とそのメカニズム解明 (2012)
  • Author(s): 滝川雅仁、曽我実、熊谷綾、平田晴実、上田学、芦田均、津田孝範
  • Organizer: 第66回日本栄養・食糧学会大会
  • Place of Presentation: 東北大学
• [Presentation] 黒大豆成分の血糖値上昇抑制作用とその作用機序 (2012)
  • Author(s): 栗本雄太、滝川雅仁、柴山勇樹、曽我実、山下陽子、 芦田均、津田孝範
  • Organizer: 第66回日本栄養・食糧学会大会
  • Place of Presentation: 東北大学
• [Presentation] Prevention of diabetes using food factors via activation of AMPK (2012)
  • Author(s): Tsuda, T.
  • Organizer: Annual Meeting of the International Society for Nutraceuticals and Functional Foods
  • Place of Presentation: Coutyard King Kamehameha Hotel, Cona, HI, USA
• [Presentation] ローヤルゼリー由来中鎖脂肪酸のAMP キナーゼ活性化作用とその機序解明 (2012)
  • Author(s): 滝川 雅仁、曽我実、熊谷綾、平田晴実、上田学、芦田均、津田孝範
  • Organizer: 日本農芸化学会2012 年度大会
  • Place of Presentation: 京都女子大学
• [Presentation] 黒大豆成分の血糖値上昇抑制作用とその作用機構の解明 (2012)
  • Author(s): 栗本 雄太、滝川 雅仁、柴山 勇樹、曽我実、山下陽子、芦田均、津田孝範
  • Organizer: 日本農芸化学会2012 年度大会
  • Place of Presentation: 京都女子大学
• [Presentation] すいおうのGLP-1 分泌促進作用と血糖値上昇抑制作用 (2012)
  • Author(s): 永峰里花、上野栞、田頭英樹、友澤寛、鍔田仁人、比良徹、原博、津田孝範
  • Organizer: 第66回日本栄養・食糧学会大会
  • Place of Presentation: 仙台
• [Presentation] 甘藷若葉末（すいおう）に含まれるGLP-1分泌促進成分に関する研究 (2012)
  • Author(s): 山田菜央、比良徹、永峰里花、鍔田仁人、津田孝範、原 博
  • Organizer: 第42 回日本栄養・食糧学会北海道支部大会
  • Place of Presentation: 帯広
• [Presentation] アントシアニンのサイエンス－「これまで」と「これから」－ (2012)
  • Author(s): 津田孝範
  • Organizer: 第1回日本アントシアニン研究会
  • Place of Presentation: 東京
  • Invited
• [Presentation] Prevention of diabetes using food factors via activation of AMPK. (2012)
  • Author(s): Tsuda, T.
  • Organizer: 2012 Annual Meeting of the International Society for Nutraceuticals and Functional Foods
  • Place of Presentation: Cona, HI, USA
• [Presentation] 黒大豆成分の血糖値上昇抑制作用とメカニズムの解明 (2011)
  • Author(s): 栗本雄太、井上聖哉、滝川雅仁、上田学、芦田均、津田孝範
  • Organizer: 第65回日本栄養・食糧学会大会
  • Place of Presentation: お茶ノ水女子大学
• [Presentation] 植物由来成分と脂肪細胞機能調節、糖尿病予防 第65 回日本栄養・食糧学会大会シンポジウム-1「肥満の基礎と臨床への展開」 (2011)
  • Author(s): 津田孝範
  • Organizer: 第65回日本栄養・食糧学会大会
  • Place of Presentation: お茶ノ水女子大学
• [Presentation] Prevention of type2 diabetes using plant-based food factors via activation of AMP-activated protein kinase (2011)
  • Author(s): Tsuda, T., Takikawa, M.
  • Organizer: 11^<th> Asian Congress of Nutrition
  • Place of Presentation: Singapore International Convention & Exibition Centre, Singapore
• [Presentation] Prevention of type2 diabetes using anthocyanin-rich berries via activation of AMP-activated protein kinase (2011)
  • Author(s): Tsuda, T.
  • Organizer: 2011International Congress on Food Factors-Food for Wellbeing-from function to processing
  • Place of Presentation: Howard Civil Service International House, Taipei, Taiwan
• [Presentation] 植物由来成分と脂肪細胞機能調節、糖尿病予防 (2011)
  • Author(s): 津田孝範
  • Organizer: 第65回日本栄養・食糧学会大会シンポジウム-1「肥満の基礎と臨床への展開」(招待講演)
  • Place of Presentation: お茶の水女子大学（東京）
• [Presentation] Prevention of type2 diabetes using plant-based food factors via activation of AMP-activated protein kinase. (2011)
  • Author(s): Tsuda, T., Takikawa, M.
  • Organizer: 11th Asian Congress of Nutrition
  • Place of Presentation: Suntec convention centter (Singapore)
• [Presentation] Brazilian propolis-derived components inhibit TNF-&#61537;-mediated downregulation of adiponectin expression via different mechanisms in 3T3-L1 adipocytes. (2011)
  • Author(s): Ikeda, R, Yanagisawa, M., Takahashi, N., Kawada, T., Kumazawa, S., Yamaotsu, N., Nakagome, I., Shuichi Hirono, S., Tsuda, T.
  • Organizer: 2011 Annual Meeting of the International Society for Nutraceuticals and Functional Foods
  • Place of Presentation: Royton Sapporo (Sapporo)
• [Presentation] Prevention of type2 diabetes using anthocyanin-rich berries via activation of AMP-activated protein kinase. (2011)
  • Author(s): Tsuda, T.
  • Organizer: 2011 International Congress on Food Factors-Food for Wellbeing-from function to processing(招待講演)
  • Place of Presentation: Taipei convention center (Taipei, Taiwan)
• [Presentation] ブラジル産プロポリスに含まれる2種の桂皮酸誘導体は、異なる機構を介してアディポネクチンの発現低下を抑制する (2011)
  • Author(s): 池田理恵、柳沢政由、高橋信之、河田照雄、熊澤茂則、山乙教之、中込泉、広野修一、津田孝範
  • Organizer: 第2回岐阜薬科大学機能性健康食品学術講演会
  • Place of Presentation: ホテルグランベール岐山（岐阜）
• [Book] ニュートリゲノミクスを基盤としたバイオマーカーの開発 (2013)
  • Author(s): 津田孝範
  • Publisher: シーエムシー出版
• [Book] ニュートリゲノミクスを基盤としたバイオマーカーの開発－未病診断とテーラーメイド食品開発へ向けて－ (2013)
  • Author(s): 津田孝範
  • Total Pages: 210
  • Publisher: シーエムシー出版
• [Book] 機能性食品の作用と安全性百科 (2012)
  • Author(s): 津田孝範
  • Total Pages: 405
  • Publisher: 丸善出版
• [Book] ポリフェノール:薬用植物および食品の機能性成分 (2012)
  • Author(s): 津田孝範
  • Publisher: シーエムシー出版
• [Book] ポリフェノール：薬用植物および食品の機能性成分 (2012)
  • Author(s): 津田孝範
  • Total Pages: 277
  • Publisher: シーエムシー出版
• [Remarks] 中部大学応用生物学部津田研究室
  • URL: http://stu.isc.chubu.ac.jp/bio/public/Food_Nutr_Sci/labo/tsuda_lab/index.html