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Functional analysis of the drought-induced LEA protein from Lombardy poplar

Research Project

Project/Area Number 23580217
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Forest science
Research InstitutionForestry and Forest Products Research Institute

Principal Investigator

NISHIGUCHI Mitsuru  独立行政法人森林総合研究所, 生物工学研究領域, 主任研究員 (80353796)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsポプラ / LEA / 環境ストレス / 遺伝子組換え / 耐塩性 / 発現機構 / 樹木 / 発現誘導
Research Abstract

To elucidate the function of LEA proteins inferred to be involved in the response to environmental stress in woody plants, the cDNAs of LEA proteins were isolated from Lombardy poplar and were investigated. Among of the poplar LEA genes, the expression of PnLEA1 was induced by drought, high salinity and low temperature. We found that PnLEA1 plays an important role for initial adaptation to salinity stress, because the expression of PnLEA1 recovered the delay in cell growth of Escherichia coli under high salinity. However, the PnLEA1-overexpressed poplar plants were withered after about 14 days without watering as well as empty vector control plants.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (8 results)

All 2014 2013 2012 Other

All Presentation (8 results)

  • [Presentation] 乾燥ストレス応答に関わるポプラのLEAタンパク質遺伝子の発現と性質2014

    • Author(s)
      西口満
    • Organizer
      第125回日本森林学会大会
    • Place of Presentation
      大宮ソニックシティ(埼玉県さいたま市)
    • Year and Date
      2014-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 水欠乏により誘導されるポプラのLEAタンパク質の機能解析2013

    • Author(s)
      西口満
    • Organizer
      第124回日本森林学会大会
    • Place of Presentation
      岩手大学(岩手県盛岡市)
    • Year and Date
      2013-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] ポプラの乾燥誘導性LEAタンパク質遺伝子の機能解析2012

    • Author(s)
      西口満
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2012-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 遺伝子組換え樹木の創出のためのポプラ環境ストレス耐性遺伝子の探索2012

    • Author(s)
      西口満、古川原聡、横田智、毛利武、田原恒、掛川弘一、楠城時彦
    • Organizer
      第123回日本森林学会大会
    • Place of Presentation
      宇都宮大学
    • Year and Date
      2012-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 遺伝子組換え樹木の創出のためのポプラ環境ストレス耐性遺伝子の探索2012

    • Author(s)
      西口満、古川原聡、横田智、毛利武、田原恒、掛川弘一、楠城時彦
    • Organizer
      日本森林学会
    • Place of Presentation
      宇都宮大学(栃木県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 乾燥ストレス応答に関わるポプラのLEAタンパク質遺伝子の発現と性質

    • Author(s)
      西口満
    • Organizer
      日本森林学会
    • Place of Presentation
      大宮ソニックシティー(埼玉県さいたま市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ポプラの乾燥誘導性LEAタンパク質遺伝子の機能解析

    • Author(s)
      西口満
    • Organizer
      日本分子生物学会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 水欠乏により誘導されるポプラのLEA タンパク質の機能解析

    • Author(s)
      西口満
    • Organizer
      日本森林学会
    • Place of Presentation
      岩手大学(岩手県盛岡市)
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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