Development of simple methods for identification of genetic leneages of Platypus quercivorus in Japan

Research Project

Project/Area Number	23580219
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Forest science
Research Institution	Forest Research and Management Organization
Principal Investigator	Hamaguchi Keiko 国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員等 (60343795)
Co-Investigator(Kenkyū-buntansha)	後藤秀章国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員等 (10353682)
Project Period (FY)	2012-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	ナラ枯れ / カシノナガキクイムシ / 遺伝的系統 / PCR-RFLP / 特異的プライマー / 地域変異 / 系統識別
Outline of Final Research Achievements	Platypus quercivorus is the vector insect of the japanese oak wilt disease.　There are several genetic leneages of the beetle in japan, however, the classification of those leneages needs taxonomist or DNA sequencer. More simple and rapid methods for identification of the beetle leneages are required to promote the study of the beetle and the damage control based on the information of the leneages. In this study, we developed the simple methods for identification of the genetic leneages of the beetle by PCR-RFLP and PCR using specific primers. In addition, we selected the effective traps to collect the beetle specimens for DNA analyses in the field.