| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
Cellulose was completely dissolved in the 60 wt% lithium bromide aqueous solution in the temperature 120 degrees. Then the cellulose solution was poured into glass container containing large excess amount of salt (NaCl) particles that was heated at 120 degrees in advance. The cellulose was regenerated from the solution by cooling down to room temperature and removing the slats by washing with water. The cellulose gel thus obtained has a uniform and large pore size more than 0.1 mm in diameter. The particle size of salts used in the process leads to the control of morphology and mechanical properties of the gel. The gels were seeded with rabbit chondrocyte and cultured in vitro for three weeks, and proliferation of chondrocyte and its differentiation to cartilagines were observed. In consequence, the cellulose gel has a possibility using as a three-dimensional scaffold for cell culture of tissue engineering.
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