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Studies on molecluar mechanism of lipid droplet formation in telesot oocytes

Research Project

Project/Area Number 23580242
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General fisheries
Research InstitutionHokkaido University

Principal Investigator

TODO TAKASHI  北海道大学, 大学院水産科学研究院, 准教授 (60303111)

Co-Investigator(Renkei-kenkyūsha) HARA Akihiko  北海道大学, 名誉教授 (40091483)
HIRAMATSU Naoshi  北海道大学, 大学院水産科学研究院, 准教授 (10443920)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords卵形成 / 卵成長 / 卵母細胞 / 油球 / 脂質 / リポタンパク質 / 卵黄 / 水産学 / タンパク質 / 魚類 / 生理学 / 水差学 / 発生・分化
Research Abstract

In teleost fishes, high amount of lipids and proteins are accumulated in oocytes during their growth phase; they are later utilized as energy and nutrient resources by developing embryos and larvae. However, little is known about the origin of such lipids and the mechanisms underlying their accumulation into oocytes. In the present study, we aimed to clarify the mechanisms at the molecular levels. As the results, we confirmed that, for the first time, plasma very low density lipoprotein is the major carrier of neutral lipid to oocyte lipid droplets, and updated the model for the oocyte lipidation.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report

Research Products

(31 results)

All 2014 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (23 results) Remarks (1 results)

  • [Journal Article] Molecular cloning and partial characterization of an ovarian receptor with seven ligand repeats, an orthologue of low-density lipoprotein receptor, in the cutthroat trout (Oncorhynchus clarki)2013

    • Author(s)
      Luo, W., Ito, Y., Mizuta, H., Massaki, K., Hiramatsu, N., Todo, T., Reading, B. J., Sullivan, C. V., Hara, A.
    • Journal Title

      Comparative Biochemistry and Physiology Part A

      Volume: 166 Pages: 263-271

    • DOI

      10.1016/j.cbpa.2013.06.026

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ovarian expression and localization of a vitellogenin receptor with eight ligand binding repeats in the cutthroat trout (Oncorhynchus clarki)2013

    • Author(s)
      Mizuta, H., Luo, W., Ito, Y., Mushirobira, Y., Todo, T., Hara, A., Reading, B. J., Sullivan, C. V., Hiramatsu, N.
    • Journal Title

      Comparative Biochemistry and Physiology, Part B

      Volume: 166 Pages: 81-90

    • DOI

      10.1016/j.cbpb.2013.07.005

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiple ovarian lipoprotein receptors in teleosts2013

    • Author(s)
      Hiramatsu N, Luo W, Reading B. J., Sullivan C. V., Mizuta H, Ryu Y. -W., Nishimiya 0, Todo T, Hara A
    • Journal Title

      Fish Physiol. Biochem

      Volume: 39 Pages: 29-32

    • DOI

      10.1007/s10695-012-9612-6

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Changes in levels of dual vitellogenin transcripts and proteins in cutthroat trout <i>Oncorhynchus clarki</i> during ovarian development2013

    • Author(s)
      莚平裕次、水田紘子、羅〓〓、盛田祐加、澤口小有美、松原孝博、平松尚志、東藤孝、原彰彦
    • Journal Title

      NIPPON SUISAN GAKKAISHI

      Volume: 79(2) Issue: 2 Pages: 175-189

    • DOI

      10.2331/suisan.79.175

    • NAID

      10031138611

    • ISSN
      0021-5392, 1349-998X
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Cloning and Transcript Expression of Genes Encoding Two Types of Lipoprotein Lipase in the Ovary of Cutthroat Trout, (Oncorhynchus clarki)2013

    • Author(s)
      Ryu, Y.W. 他7名
    • Journal Title

      Zoological Science

      Volume: 39(2) Issue: 3 Pages: 224-237

    • DOI

      10.2108/zsj.30.224

    • NAID

      40019603064

    • ISSN
      0289-0003
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of vitellogenin and its derived yolk proteins in cloudy catshark(Scyliorhinus torazame).2013

    • Author(s)
      Yamane K, Yagai T, Nishimiya O, Sugawara R, Amano H, Fujita T, Hiramatsu N, Todo T, Matsubara T, Hara A.
    • Journal Title

      Fish Physiol Biochem

      Volume: 39(2) Pages: 373-390

    • DOI

      10.1007/s10695-012-9706-1

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] カットスロートトラウトOncorhynchus clarkiにおける2型ビテロジェニン転写産物および蛋白質の卵発達に伴う発現変化.2013

    • Author(s)
      筵平裕次・水田紘子・羅ブンシュ・盛田祐加・澤口小有美・松原孝博・平松尚志・東藤孝・原彰彦
    • Journal Title

      日本水産学会誌

      Volume: 79(2) Pages: 175-189

    • NAID

      10031138611

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] カットスロートトラウト(Oncorhynchus clarki)における血漿リボ蛋白質の分離とアポリポ蛋白質Eの検出2014

    • Author(s)
      筵平裕次
    • Organizer
      平成26年度日本水産学会春季大会
    • Place of Presentation
      北海道大学函館キャンパス(北海道函館市)
    • Related Report
      2013 Final Research Report
  • [Presentation] カットスロートトラウト(Oncorhynchus clarki)における血漿リポ蛋白質の分離とアポリポ蛋白質Eの検出2014

    • Author(s)
      筵平裕次・竹下眞広・東藤孝・原彰彦・平松尚志
    • Organizer
      平成26年度日本水産学会春季大会
    • Place of Presentation
      北海道大学函館キャンパス(北海道函館市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ligand binding properties of ovarian lipoprotein receptors in the cutthroat trout (Oncorhynchus clarki)2013

    • Author(s)
      Yuji Mushirobira
    • Organizer
      The 10^<th> International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
    • Place of Presentation
      Jeju National University, Jeju, Korea
    • Related Report
      2013 Final Research Report
  • [Presentation] Yolk formation in fish: Multiple vitellogenins and their receptors2013

    • Author(s)
      Naoshi Hiramatsu
    • Organizer
      Diversification in Inland Finfish Aquaculture II (DIFAII)
    • Place of Presentation
      University of South Bohemia, Vodnany, Czech Republic
    • Related Report
      2013 Final Research Report
  • [Presentation] サケ科魚類のビテロジェニンに結合するリポ蛋白質受容体の探索と性状解析2013

    • Author(s)
      筵平裕次
    • Organizer
      平成25 年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都品川区)
    • Related Report
      2013 Final Research Report
  • [Presentation] カットスロートトラウト卵巣におけるクラスリン重鎖転写産物および同翻訳産物の局在に関する組織学的解析2013

    • Author(s)
      水田紘子
    • Organizer
      平成25 年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都品川区)
    • Related Report
      2013 Final Research Report
  • [Presentation] Yolk formation in fish: Multiple vitellogenins and their receptors.2013

    • Author(s)
      Hiramatsu, N., Mizuta, H., Luo, W., Nishimiya, O., Wu, Meiqin, Mushirobira, Y., Reading, B.J., Sullivan, C.V., Todo, T., Hara, A.
    • Organizer
      Diversification in Inland Finfish Aquaculture II (DIFAII)
    • Place of Presentation
      University of South Bohemia, Vodnany, Czech Republic
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ligand binding properties of ovarian lipoprotein receptors in the cutthroat trout (Oncorhynchus clarki).2013

    • Author(s)
      Mushirobira, Y., Mizuta, H., Luo, W., Todo, T., Hara, A., Hiramatsu, N.
    • Organizer
      The 10th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
    • Place of Presentation
      Jeju National University, Jeju, Korea
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヌタウナギの2種エストロジェン受容体の機能解析.2013

    • Author(s)
      西宮攻・勝義直・稲川裕之・平松尚志・東藤孝・原彰彦
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] サケ科魚類のビテロジェニンに結合するリポ蛋白質受容体の探索と性状解析.2013

    • Author(s)
      筵平裕次・水田紘子・羅ブンシュ・東藤孝・原彰彦・平松尚志
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] カットスロートトラウト卵巣におけるクラスリン重鎖転写産物および同翻訳産物 の局在に関する組織学的解析.2013

    • Author(s)
      水田紘子・東藤孝・原彰彦・平松尚志
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] カットスロートトラウト卵巣における常染色体劣勢高コレステロール血症原因蛋白質(ARH)のcDNA クローニング及び遺伝子発現解析2012

    • Author(s)
      水田紘子
    • Organizer
      平成24 年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都品川区)
    • Related Report
      2013 Final Research Report
  • [Presentation] Molecular clonig and characterization of two estrogen subtypes form a hagfish, Eptatretus burger.2012

    • Author(s)
      Nishimiya O., Inagawa H., Katsu Y., Hiramatsu N., Todo T., Hara A.
    • Organizer
      The 9th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
    • Place of Presentation
      長崎大学東京オフィス(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] Dual vitellogenin in cutthroat trout (Oncorhynchus clarki): purification and changes in serum protein levels during reproductive cycle.2012

    • Author(s)
      Mushirobira Y., Mizuta H., Luo W., Morita Y., Sawaguchi S., Matsubara T., Hiramatsu N., Todo T., Hara A
    • Organizer
      The 9th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
    • Place of Presentation
      長崎大学東京オフィス(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] カットスロートトラウトにおける常染色体劣勢高コレステロール血症原因蛋白質(ARH)のcDNAクローニング及び遺伝子発現解析2012

    • Author(s)
      水田紘子
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都品川区)
    • Related Report
      2011 Research-status Report
  • [Presentation] Expression of genes involved in oocyte lipidation in cutthroat trout, Oncorhynchus clarki2011

    • Author(s)
      Yong-Woon Ryu
    • Organizer
      9^<th> International Symposium on Reproductive Physiology of Fish
    • Place of Presentation
      Lulu Convention Centre, Cochin, India
    • Related Report
      2013 Final Research Report
  • [Presentation] Molecular cloning and localization of two classical ovarian lipoprotein receptors in cutthroat trout, Oncorhynchus clarki2011

    • Author(s)
      Hiroko Mizuta
    • Organizer
      9^<th> International Symposium on Reproductive Physiology of Fish
    • Place of Presentation
      Lulu Convention Centre, Cochin, India
    • Related Report
      2013 Final Research Report
  • [Presentation] Multiple ovarian lipoprotein receptors in teleosts2011

    • Author(s)
      Naoshi Hiramatsu
    • Organizer
      9^<th> International Symposium on Reproductive Physiology of Fish
    • Place of Presentation
      Lulu Convention Centre, Cochin, India
    • Related Report
      2013 Final Research Report
  • [Presentation] A Novel class of ovarian liporotein receptor in cutthroat trout: Molecular cloning and expression analysis.2011

    • Author(s)
      Naoshi Hiramatsu
    • Organizer
      9th International Symposium on Reproductive Physiology of Fish(招待講演)
    • Place of Presentation
      Lulu Convention Centre, Cochin, India
    • Related Report
      2011 Research-status Report
  • [Presentation] Molecular cloning and localization of two classical ovarian lipoprotein receptors in cutthroat trout Oncorhynchus Clarki.2011

    • Author(s)
      Hiroko Mizuta
    • Organizer
      9th International Symposium on Reproductive Physiology of Fish
    • Place of Presentation
      Lulu Convention Centre, Cochin, India
    • Related Report
      2011 Research-status Report
  • [Presentation] Expression of genes involved in oocyte lipidation in cutthroattrout, Onchorhychus clarki.2011

    • Author(s)
      Yon-Woon Ryu
    • Organizer
      9th International Symposium on Reproductive Physiology of Fish
    • Place of Presentation
      Lulu Convention Centre, Cochin, India
    • Related Report
      2011 Research-status Report
  • [Presentation] カットスロートトラウト(Oncorhychus clarki)における2型ビテロジェニン遺伝子の発現解析2011

    • Author(s)
      莚平裕次
    • Organizer
      平成23年度日本水産学会北海道支部大会
    • Place of Presentation
      北海道大学(函館市)
    • Related Report
      2011 Research-status Report
  • [Presentation] カットスロートトラウト卵巣におけるリポ蛋白質受容体の局在解析2011

    • Author(s)
      水田紘子
    • Organizer
      平成23年度日本水産学会北海道支部大会
    • Place of Presentation
      北海道大学(函館市)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.geocities.jp/hlaboratory/top.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-04   Modified: 2019-07-29  

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