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Investigation of resistance mechanism of ayu against cold water disease by comprehensive analysis of antimicrobial peptides

Research Project

Project/Area Number 23580254
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General fisheries
Research InstitutionHiroshima University

Principal Investigator

IIJIMA NORIAKI  広島大学, 生物圏科学研究科, 教授 (90136143)

Co-Investigator(Kenkyū-buntansha) FURUSAWA Shuichi  広島大学, 大学院生物圏科学研究科, 教 授 (80130037)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords魚病 / 生体防御 / 抗菌ペプチド / アユ / 魚病細菌 / 殺菌効果 / 自然免疫
Research Abstract

We report here the isolation of two novel antimicrobial peptides from the spleen of Ayu, Plecoglossus altivelis. Antimicrobial peptides were purified from the acid extract of Ayu spleen with ion-exchange chromatography, gel-filtrarion chromatography, and two-step reversed-phase HPLC. Peptide sequences were determined by a combination of Edman degradation and ESI mass spectrometry. Two antimicrobial peptides, named plecoglosin-1 and -2, consist of 11 and 10 amino acids, respectively. Synthetic plecoglosin-1 and -2 displayed broad-spectrum bactericidal activity against Gram-negative and Gram-positive bacteria including Escherichia coli, Bacillus subtilis, and fish and crustacean pathogens.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (4 results)

All 2013 2012

All Presentation (4 results)

  • [Presentation] マダイ及びアユ抗菌ペプチド遺伝子のクローニングと発現2013

    • Author(s)
      濱野太肇・乗富修平・国吉久人・藤川愉吉・飯島憲章(広大院生物圏)
    • Organizer
      日本水産学会中国・四国支部例会
    • Place of Presentation
      宇和島市総合福祉センター
    • Year and Date
      2013-11-16
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] アユ表皮粘液における抗菌ペプチドの網羅的解析2013

    • Author(s)
      坂口裕紀・山口翔(広大院生物圏)・網本智子(広大技術センター)・内田直行(日大生物資源)・飯島憲章(広大院生物圏)
    • Organizer
      日本水産学会中国・四国支部例会
    • Place of Presentation
      宇和島市総合福祉センター
    • Year and Date
      2013-11-16
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] アユ脾臓組織における抗菌ペプチドの網羅的解析2012

    • Author(s)
      藤本実希・青木翼(広大院生物圏)・網本智子(広大技術センター)・内田直行(日生物資源)・影山哲史(岐阜県河川研)・飯島憲章(広大院生物圏)
    • Organizer
      日本水産学会中国・四国支部例会
    • Place of Presentation
      福山大学工学部
    • Year and Date
      2012-12-01
    • Related Report
      2013 Final Research Report
  • [Presentation] アユ脾臓組織における 抗菌ペプチドの網羅的解析2012

    • Author(s)
      藤本実希・青木 翼・ 網本智子・内田直行・影山哲史・飯島憲章
    • Organizer
      日本水産学会中国・四国支部例会
    • Place of Presentation
      福山大学
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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