Microbioassay for the analysis of toxic factors produced by red tide phytoplankton
| Project/Area Number |
23580255
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Nagasaki University |
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Principal Investigator |
ODA Tatsuya 長崎大学, 水産・環境科学総合研究科, 教授 (60145307)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Kenichi 長崎大学, 水産・環境科学総合研究科, 准教授 (90363473)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | プランクトン / 赤潮 / 毒性 / 培養細胞 / バイオアッセイ / 細胞培養 |
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| Research Abstract |
Harmful algal blooms (HABs) are one of serious environmental problems. To investigate the toxic mechanism of HABs species, direct exposure experiments against fish or shellfish have been conducted. However, such exposure experiments generally require special animal rearing facilities and mass-culture of phytoplankton. Usage of appropriate cultured cells as an alternative for whole living animals seems to be a promising strategy. We evaluated lactate dehydrogenase (LDH)-release assay as a simple estimation method for toxic potential of harmful algal species at cellular level. Live cell suspension of Karenia mikimotoi (strain SUO-1), which is highly toxic to rotifer and has potent hemolytic activity, induced the release of significant levels of LDH from all the cell lines tested in cell-density and time-dependent manners, whereas another strain of K. mikimotoi (strain FUK), which is less-toxic to rotifer and has low hemolytic activity, showed fairly low activities.
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] Application of LDH-Release Assay to Cellular-Level Evaluation of the Toxic Potential of Harmful Algal Species2013
Author(s)
Yanan Zou, Daekyung Kim, Motoaki Yagi, Yasuhiro Yamasaki, Jun Kurita, Takaji Iida, Yukihiko Matsuyama, Kenichi Yamaguchi, and Tatsuya Oda
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 77
Issue: 2
Pages: 345-352
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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[Journal Article] Quercitrin protects against ultraviolet B-1 induced cell death in vitro and in an in vivo zebrafish model2012
Author(s)
Hye-Mi Yang, Young-Min Ham, Weon-Jong Yoon, Seong Woon Roh, You-Jin Jeon, Tatsuya Oda,Sung-Myung Kang, Min-Cheol Kang, Eun-A Kim, Daekyung Kim, Kil-Nam Kim
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Journal Title
Journal of Photochemistry and Photobiology B
Volume: 114
Pages: 126-131
DOI
Related Report
Peer Reviewed
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[Journal Article] Neuroprotective Effects of Nannochloropsis oculata Against AAPH-induced Oxidative DNA Damage in HT22 Cells2012
Author(s)
Kil-Nam Kim, Seon-Heui Cha, Eun-A Kim, Min-Cheol Kang, Hye-Mi Yang, Min-Joo Kim, Hye-Young Yang, Seong Woon Roh, Won-Kyo Jung, Soo-Jin Heo, Daekyung Kim, You-Jin Jeon, and Tatsuya Oda
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Journal Title
International Journal of Pharmacology
Volume: 8
Issue: 6
Pages: 527-534
DOI
Related Report
Peer Reviewed
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[Journal Article] Cell contanct-dependent lethal effect of the dinoflagellate Heterocapsa circularisquama on phytoplankton-phytoplankton interaction2011
Author(s)
Y. Yamasaki, Y. Zou, J. Go, T. Shikata, Y. Matsuyama, K. Nagai, Y. Shimasaki, K. Yamaguchi, Y. Oshima, T. Oda, T. Honjo
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Journal Title
Journal of Sea Research
Volume: 65
Pages: 76-83
Related Report
Peer Reviewed
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[Journal Article] Cell contanct-dependent lethal effect of the dinoflagellate Heterocapsa circularisquama on phytoplankton-phytoplankton interaction.2011
Author(s)
Yasuhiro Yamasaki, Yanan Zou, Jyoji Go, Tomoyuki Shikata, Yukihiko Matsuyama, Kiyohito Nagai, Yohei Shimasaki, Kenichi Yamaguchi, Yuji Oshima, Tatsuya Oda, Tsuneo Honjo.
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Journal Title
Journal of Sea Research.
Volume: 65
Issue: 1
Pages: 76-83
DOI
Related Report
Peer Reviewed
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[Journal Article] Comparative Study on the Toxic Effects of Red Tide Flagellates Heterocapsa circularisquama and Chattonella marina on the Short-Necked Clam (Ruditapes philippinarum)2011
Author(s)
Daekyung Kim, Kwang-Sik Choi, Hyun-Ki Hong,Zedong Jiang, Yanan Zou, Kyu-Sung Choi, Yasuhiro Yamasaki, Yukihiko Matsuyama, Kenichi Yamaguchi, and Tatsuya Oda.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 75
Issue: 10
Pages: 2052-2055
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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