| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
|
|---|
| Research Abstract |
To monitor the expression of cytokine genes in Japanese pufferfish, a novel platform for quantitative multiplexed analysis was developed. This custom-designed multiplex RT-PCR assay was used to analyze the expression profiles of 19 cytokine genes, including pro-inflammatory, anti-inflammatory, T-cell proliferation/differentiation, B-cell activation/differentiation, NK cell stimulation, induction of anti-viral activity, and monocyte/macrophage progenitor cell proliferation cytokines in head kidney cells under immune stimulatory conditions. The expression profiles were dissimilar in the unstimulated control and immune-stimulated cells. Moreover, increased expression profile was observed due to different stimulations for IL-1b, IL-6, IL-10, IL-12p35, IL-12p40, IL-21, TNF-a, TNF-N, I-IFN-1 and IFN-g genes. These results suggest that cytokine genes could be used as biomarkers to know the innate immune status of fish.
|
