The establishment of the method for cell culture and a study on the function of a new aquaporin in dolphins

• Project/Area Number: 23580265
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General fisheries
• Research Institution: Nihon University
• Principal Investigator: SUZUKI Miwa 日本大学, 生物資源科学部, 講師 (70409069)
• Co-Investigator(Kenkyū-buntansha): ITOU Takuya 日本大学, 生物資源科学部, 伊藤 琢也(ITOU, TAKUYA) 日本大学・生物資源科学部・准教授 研究者番号:20307820 (20307820)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 鯨類 / 浸透圧調節 / 細胞培養 / アクアポリン / 海水適応 / イルカ / 浸透圧耐性 / ノックダウン / バンドウイルカ / 浸透圧

Research Abstract

Our previous data suggested alternative splicing of aquaporin (AQP) -2 in bottle nose dolphins. As aqp2 gene has response element to hypertonicity, we hypothesized the alternatively spiced-AQP2 (AQP2') has some functions for cellular osmoregulation. Experiments were carried out to test the hypothesis and interesting results were obtained: (1) Culture technique of primary cells from several organs of the dolphin was established. (2) AQP2 was expressed only in the kidney, whereas AQP2' expression was confirmed in all organs tested. (3) Genomic analyses showed AQP2' sequence is highly and broadly conserved in cetacean species, but not in other mammals. (4) Expression of AQP2 and AQP2' were increased in response to increase in Na+ concentration in medium. (5) Knockdown for AQP2 and AQP2' by small interfering RNA resulted in severe shrinkage and lowering cellular viability in dolphins' cells. Collectively, there data suggested AQP2' may contribute to cellular osmotic tolerance.

Research Products

• [Journal Article] Activation of systemic, but not local, renin-angiotensin system is associated with up-regulation of TNF-αduring prolonged fasting in northern elephant seal pups (2013)
  • Author(s): Suzuki M, Vázquez-Medina JP, Viscarra JA, Soñanez-Organis JG, Crocker DE, Ortiz RM.
  • Journal Title: Journal of Experimental Biology Volume: 216 Pages: 3215-3221
  • Peer Reviewed
• [Journal Article] Molecular characterization and validation of commercially available methods for haptoglobin measurement in bottlenose dolphin (2013)
  • Author(s): Segawa T, Amatsuji H, Suzuki K, Suzuki M, Yanagisawa M, Itou T, Sakai T, Nakanishi T.
  • Journal Title: Results in Immunology Volume: 3 Pages: 57-63
  • Peer Reviewed
• [Journal Article] Characterization of the circulating serum amyloid A in bottlenose dolphins (2013)
  • Author(s): Segawa T, Otsuka T, Itou T, Suzuki M, Karatani N, Sakai T.
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 152 Pages: 218-224
  • Peer Reviewed
• [Journal Article] Activation of systemic, but not local, renin-angiotensin system is associated with up-regulation of TNF-α during prolonged fasting in northern elephant seal pups. (2013)
  • Author(s): Suzuki M, Vazquez-Medina JP, Viscarra JA, Sonanez-Organis JG, Crocker DE, Ortiz RM
  • Journal Title: J. Exp. Biol. Volume: 216 Pages: 3215-3221
  • DOI: 10.1242/jeb.085225
  • Peer Reviewed
• [Journal Article] Secretory patterns of catecholamines in Indo-Pacific bottlenose dolphins (2012)
  • Author(s): Suzuki M, Nozawa A, Ueda K, Bungo T, Terao H, Asahina K.
  • Journal Title: General and Comparative Endocrinology Volume: 177 Pages: 76-81
  • Peer Reviewed
• [Journal Article] Hematopoietic cell populations in dolphin bone marrow : Analysis of colony formation and differentiation (2011)
  • Author(s): Segawa T, Itoh T, Suzuki M, Moritomo T, Nakahishi T, Sakai T.
  • Journal Title: Results in Immunology Volume: 1 Pages: 1-5
  • Peer Reviewed
• [Presentation] Activation of systemic renin-angiotensin system is a causal factor of insulin resistance mediated by TNF-αupregulation & Acrp30 suppression in post-weaning fasted northern elephant seal pups? (2013)
  • Author(s): Suzuki M, Vázquez-Medina JP, Viscarra JA, Soñanez-Organis JG, Crocker DE, Ortiz RM.
  • Organizer: The 20th Biennial Conference of the Biology of Marine Mammals
  • Place of Presentation: Dunedin, NZ
  • Year and Date: 2013-12-12
• [Presentation] 絶食中のキタゾウアザラシのRASおよび免疫活性とインシュリン抵抗性 (2013)
  • Author(s): 鈴木美和, Vázquez-Medina JP, Viscarra JA, Soñanez-Organis JG, Crocker DE, Ortiz RM.
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学,品川,東京
  • Year and Date: 2013-03-26
• [Presentation] Activation of systemic renin-angiotensin system is a causal factor of insulin resistance mediated by TNF-α upregulation & Acrp30 suppression in post-weaning fasted northern elephant seal pups? (2013)
  • Author(s): Suzuki M, Vazquez-Medina JP, Viscarra JA, Sonanez-Organis JG, Crocker DE, Ortiz RM
  • Organizer: The 20 th Biennial Conference of the Biology of Marine Mammals
  • Place of Presentation: Otago University, Dunedin, New Zealand
• [Presentation] Secretion and function of arginine-vasopressin in bottlenose dolphin (2012)
  • Author(s): Suzuki M, Masaki K, Tsurumaki H, Asahina K
  • Organizer: The 19th Biennial Conference on the Biology of Marine Mammals
  • Place of Presentation: Florida, USA
• [Presentation] Secretion and function of arginine-vasopressin in bottlenose dolphin (2011)
  • Author(s): Suzuki M, Masaki K, Tsurumaki H, Itou T.
  • Organizer: The 19th Biennial Conference on the Biology of Marine Mammals
  • Place of Presentation: Florida, USA
  • Year and Date: 2011-11-29
• [Book] 水族館と海の生き物たち (2014)
  • Author(s): 杉田治男（編）
  • Total Pages: 193
  • Publisher: 恒星社厚生閣
• [Book] "海獣・鳥類のしくみ"『水族館と海の生き物たち』,杉田治男編著,第14章 (2012)
  • Author(s): 鈴木美和
  • Publisher: 東海大学出版会
• [Book] "鯨類は水を飲まないのか?—浸透圧調節—"『ケトスの知恵』,村山司・森阪匡通編著 (2012)
  • Author(s): 鈴木美和
  • Publisher: 東海大学出版会
• [Book] ケトスの知恵 (2012)
  • Author(s): 村山司，森阪匡通（編）
  • Total Pages: 212
  • Publisher: 東海大学出版会