| Project/Area Number |
23580273
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Fisheries Research Agency |
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Principal Investigator |
OKUZAWA Koichi 独立行政法人水産総合研究センター, 増養殖研究所, 主幹研究員 (00211813)
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| Co-Investigator(Kenkyū-buntansha) |
GEN Koichiro 水産総合研究, 西海区水産研究 所, 主任研究員 (80372051)
KAZETO Yukinori 水産総合研究, 増養殖研究所, 主任研究員 (60399996)
TANAKA Hideki 水産総合研究, 増養殖研究所, グループ長 (00372029)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | マダイ / 生殖腺刺激ホルモン / ラジオイムノアッセイ / 組換えタンパク / 濾胞刺激ホルモン / 黄体形成ホルモン / 初回成熟 / puberty / 組換えホルモン / 生殖線刺激ホルモン |
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| Research Abstract |
The two gonadotropins, follicle stimulating hormone (FSH) and luteinizing hormone (LH), play pivotal roles in the puberty in fish. We have newly established the method for measuring the FSH of the red seabream and elucidated the seasonal changes of FSH concentrations in the plasma of the red seabream with this method. Also, we found that gonadotropin-releasing hormone stimulates LH secretion but does not stimulate FSH secretion from the pituitary in the red seabream through the several in vivo and in vitro studies. In addition, we have established two stable cell lines that secrete FSH and LH of the red seabream, respectively and succeeded in obtaining sufficient amount of recombinant hormones (30 - 60 mg) that can be utilized for the studies for artificial induction of sexual maturation in fish.
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