Study of characteristics and control of bacteriocin-resistant Listeria monocytogenes in seafood
| Project/Area Number |
23580274
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
YAMAZAKI KOJI 北海道大学, 大学院水産科学研究院, 准教授 (40250500)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | リステリア / ナイシン / バクテリオシン / 耐性菌 / リステリア菌 |
|---|
| Research Abstract |
This study was investigated the characterization of bacteriocin-resistant Listeria monocytogenes and the development of inhibition method using bacteriocin (nisin A) against L.monocytogenes in seafood products. Nisin-resistant L.monocytogenes showed that the affinity between the cell surface of the resistant strains and nisin molecule was reduced, compared with those of wild strains. And protein profiles on the cytoplasmic membrane and the structure of peptidoglycan layer of the nisin-resistant strains would be also changed. The combination of nisin A and pectin digests was effective for the control of L.monocytogenes growth in raw salmon roes seasoned with soy-sauce at refrigeration temperature, suggesting that the combination of them will be a promising tool to ensure food safety to L.monocytogenes contamination in seafood.
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Report
(4 results)
Research Products
(12 results)
