| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
Growth in fish is regulated by the growth hormone (GH)-insulin-like growth factor (IGF) axis. Little is known concerning the in vivo levels of global metabolites and the mechanism of enhancement of growth in GH transgenic fish. The present study examined the charged metabolites levels in GH transgenic coho salmon (Oncorhynchus kisutch) by metabolomic analysis.The most notable difference found between transgenic and NT fish was that glycolysis metabolite levels changed in the muscle of transgenic fish. In addition, the change in some metabolite levels in the transgenic fish muscle was enhanced by ration-restriction. However, a few metabolites levels of the TCA cycle were found to decrease in the transgenic fish muscle. These effects observed in muscle were different from effects seen in liver. The results suggest that GH transgenesis can improve the use of carbohydrates as a source of energy associated with rapid growth.
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