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Toxicity and food safety assessment of hybrid pufferfish

Research Project

Project/Area Number 23580283
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Fisheries chemistry
Research InstitutionNagasaki University

Principal Investigator

TAKATANI Tomohiro  長崎大学, 大学院水産・環境科学総合研究科, 教授 (90304972)

Co-Investigator(Kenkyū-buntansha) ARAKAWA Osamu  長崎大学, 大学院水産・環境科学総合研究科, 教授 (40232037)
Co-Investigator(Renkei-kenkyūsha) SUZUKI Shigenori  独立行政法人水産総合研究センター, 増養殖研究所, 主任研究員 (60463105)
MOCHIOKA Noritaka  九州大学, 大学院農学研究院, 准教授 (40212261)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords食品衛生 / フグ毒(TTX) / 交雑フグ / 人工交雑フグ / フグ毒
Research Abstract

We examined to clarify the toxicity, toxin accumulation ability, and the inherited characteristics of hybrid pufferfish, as well as the transfer/accumulation/elimination mechanisms of TTX in the pufferfish body. 20 natural hybrid specimens were collected from coastal waters of Japan, and their parent species were identified by analysis of those mitochondrial and nuclear DNA. Moreover, anatomical distributions of toxicity were clarified by LC/MS analysis. Five hybrids species were contained in 20 specimens, and they were identified as "shimatora", "magoma", "matora", "toragoma" and "torama", respectively. When TTX was administrated to artificial hybrid "torama" and "torakusa", transferred first to the liver and then to the skin and ovary. On the other hand, TTX was transferred also to the testis in male "kusatora".

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (17 results)

All 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (10 results)

  • [Journal Article] 天然トラフグ肝臓の毒性分布2013

    • Author(s)
      谷口香織,高尾秀樹,新名真也,山中祐二,岡田行長,中島梨花,王俊杰,辰野竜平,阪倉良孝,高谷智裕,荒川修,野口玉雄
    • Journal Title

      食品衛生学雑誌

      Volume: 54 Pages: 128-133

    • NAID

      130003369303

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Change in the transfer profile of orally administered tetrodotoxin to non-toxic cultured pufferfish Takifugu rubripes depending of its development stage2013

    • Author(s)
      R. Tatsuno, M. Shikina, Y. Shirai, J. Wang, K. Soyano, G. N. Nishihara, T. Takatani, O. Arakawa
    • Journal Title

      Toxicon

      Volume: 65 Pages: 76-80

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Difference in the localization of tetrodotoxin between the female and male pufferfish Takifugu niphobles, during spawning2012

    • Author(s)
      S. Itoi, S. Yoshikawa, R. Tatsuno, M. Suzuki, K. Asahina, S. Yamamoto, S. Takanashi, T. Takatani, O. Arakawa, Y. Sakakura, H. Sugita
    • Journal Title

      Toxicon

      Volume: 60 Pages: 1000-1004

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tetrodotoxin Poisoning Due to Smooth-backed Blowfish Lagocephalus inermis and Toxicity of L. inermis Caught off the Kyushu Coast, Japan2012

    • Author(s)
      Y. Nagashima, T. Matsumoto, K. Kadoyama, S. Ishizaki, S. Taniyama, T. Takatani, O. Arakawa, M. Terayama
    • Journal Title

      J. Food. Hyg. Soc. Japan

      Volume: 53 Pages: 85-90

    • NAID

      130002109178

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transfer Profile of Orally and Intramuscularly Administered Tetrodotoxin to Artificial Hybrid Specimens of the Pufferfish Takifugu rubripes and Takifugu porphyreus2012

    • Author(s)
      J-J. Wang, T. Araki, R. Tatsuno, S. Nina, K. Ikeda, T. Takatani, O. Arakawa
    • Journal Title

      J. Food. Hyg. Soc. Japan

      Volume: 53 Pages: 33-38

    • NAID

      130002109402

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transfer Profile of Orally and Intramuscularly Administered Tetrodotoxin to Artificial Hybrid Specimens of the Pufferfish Takifugu rubripes and Takifugu porphyreus.2012

    • Author(s)
      J-J. Wang, T. Araki, R. Tatsuno, S. Nina, K. Ikeda, T. Takatani, O. Arakawa
    • Journal Title

      Food Hygiene and Safety Science

      Volume: 53 Pages: 33-38

    • NAID

      130002109402

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transfer profile of intramuscularly administered tetrodotoxin to artificial hybrid specimens of pufferfish, Takifugu rubripes and Takifugu niphobles2011

    • Author(s)
      J-J. Wang, T. Araki, R. Tatsuno, S. Nina, K. Ikeda, M. Hamasaki, Y. Sakakura, T. Takatani, O. Arakawa
    • Journal Title

      Toxicon

      Volume: 58 Pages: 565-569

    • NAID

      120006985277

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 天然トラフグ肝臓の毒性分布2013

    • Author(s)
      谷口香織,高尾秀樹,新名真也,山中祐二,岡田行長,中島梨花,王俊杰,辰野竜平,高谷智裕,荒川修,野口玉雄
    • Organizer
      日本食品衛生学会
    • Place of Presentation
      沖縄コンベンションセンター,沖縄
    • Related Report
      2013 Final Research Report
  • [Presentation] テトロドトキシンを給餌した腐肉食性小型巻貝2種の毒性2013

    • Author(s)
      辰野竜平,反町太樹,谷山茂人,大城直雅,久保弘文,高谷智裕,荒川修
    • Organizer
      日本食品衛生学会
    • Place of Presentation
      沖縄コンベンションセンター,沖縄
    • Related Report
      2013 Final Research Report
  • [Presentation] 天然ヒガンフグの麻痺性貝毒蓄積能2013

    • Author(s)
      山中祐二,新名真也,山下洋平,辰野竜平,高谷智裕,荒川修
    • Organizer
      日本食品衛生学会
    • Place of Presentation
      沖縄コンベンションセンター,沖縄
    • Related Report
      2013 Final Research Report
  • [Presentation] 天然ヒガンフグの麻痺性貝毒蓄積能2013

    • Author(s)
      山中祐二,新名真也,山下洋平,辰野竜平,高谷智裕,荒川 修
    • Organizer
      日本食品衛生学会
    • Place of Presentation
      沖縄(沖縄コンベンションセンター)
    • Related Report
      2013 Annual Research Report
  • [Presentation] テトロドトキシンを給餌した腐肉食性小型巻貝2種の毒性2013

    • Author(s)
      辰野竜平,反町太樹,谷山茂人,大城直雅,久保弘文,高谷智裕,荒川 修
    • Organizer
      日本食品衛生学会
    • Place of Presentation
      沖縄(沖縄コンベンションセンター)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 天然トラフグ肝臓の毒性分布2013

    • Author(s)
      谷口香織,髙尾秀樹,新名真也,山中祐二,岡田幸長,中島梨花,王 俊杰,辰野竜平, 阪倉良孝,高谷智裕,荒川 修,野口玉雄
    • Organizer
      日本食品衛生学会
    • Place of Presentation
      沖縄(沖縄コンベンションセンター)
    • Related Report
      2013 Annual Research Report
  • [Presentation] クサフグ×トラフグ人工交雑個体に筋肉内投与したテトロドトキシンの動態2012

    • Author(s)
      新名真也,荒木泰一朗,王俊杰,辰野竜平,濱崎将臣,山中祐二,高谷智裕,荒川修
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学,東京
    • Related Report
      2013 Final Research Report
  • [Presentation] トラフグ×マフグ人工交雑個体の消化管および筋肉内に投与したテトロドトキシンの動態2012

    • Author(s)
      王俊杰,荒木泰一朗,辰野竜平,新名真也,池田光壱,高谷智裕,荒川修
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学,東京
    • Related Report
      2013 Final Research Report
  • [Presentation] トラフグ×マフグ人工交雑個体の消化管および筋肉内に投与したテトロドトキシンの動態

    • Author(s)
      王俊杰,荒木泰一朗,辰野竜平,新名真也,池田光壱,高谷智裕,荒川修
    • Organizer
      日本水産学会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] クサフグ×トラフグ人工交雑個体に筋肉内投与したテトロドトキシンの動態

    • Author(s)
      新名真也,荒木泰一朗,王俊杰,辰野竜平,濱崎将臣,山中祐二,高谷智裕,荒川修
    • Organizer
      日本水産学会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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