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A study on ASP toxin production and dynamics in toxin producing organisms

Research Project

Project/Area Number 23580285
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Fisheries chemistry
Research InstitutionKitasato University

Principal Investigator

KOTAKI Yuichi  北里大学, 海洋生命科学部, 准教授 (30113278)

Co-Investigator(Kenkyū-buntansha) KOBIYAMA Atsushi  北里大学, 海洋生命科学部, 准教授 (60337988)
YASUMOTO Ko  北里大学, 海洋生命科学部, 講師 (00448200)
TERADA Ryuta  鹿児島大学, 水産学部, 准教授 (70336329)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords記憶喪失性貝毒 / ドウモイ酸 / イソドウモイ酸 / Pseudo-nitzschia / Nitzschia / navis-varingica / ハナヤナギ / 黒潮 / 細菌 / 分布特性 / 珪藻 / multiseries
Research Abstract

Distribution, ASP toxin production characteristics and the dynamics of these toxins were surveyed for the toxin producing diatom, Pseudo-nitszchia multiseries, Nitzschia navis-varingica and red alga Chondria armata. It was hypothesized that N. navis-varingica is a tropical species and expanded its distribution to eastern Japan along the warm water current "Kuroshio" which flows from the east coast of the Philippines to Japan. The characteristics of growth and the geographical comparison in the toxin profiles and genetic markers in N. navis-varingica strains along the "Kuroshio" supported the above hypothesis. N. navis-varingica shows the toxin profiles composed of domoic acid and isodomoic acids A and B. Factors controlling the toxin profiles were searched, resulting in finding the important roles of genetic difference and environmental bacteria. Bacterial effect on the toxin production seemed to be the most obvious in P. multiseries culture than in other organisms cultures.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (21 results)

All 2014 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (10 results) Book (1 results)

  • [Journal Article] Morphology and molecular phylogeny of Nitzschia bizertensis sp. nov. - a new domoic acid-producer2014

    • Author(s)
      Donia Bouchouicha Smida, Nina Lundholm, Wiebe H.C.F. Kooistra, Inès Sahraoui, Maria Valeria Ruggiero, Yuichi Kotaki, Marianne Ellegaard, Christophe Lambert, Hassine Hadj Mabrouk, Asma Sakka Hlail
    • Journal Title

      Harmful Algae

      Volume: 32 Pages: 49-63

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Morphology and molecular characterization of Pseudo-nitzschia (Bacillariophyceae) from Malaysian Borneo, including thenew species Pseudo-nitzschia circumpora sp. nov2012

    • Author(s)
      Hong-Chang Lim, Chui-Pin Leaw, Suriyanti Nyun-Pau Su, Sing-Tung Teng, Gires Usup, Normawaty Mohammad-Noor, Nina Lundholm, Yuichi Kotaki, Po-Teen Lim
    • Journal Title

      J. Phycol.

      Volume: 48 Issue: 5 Pages: 1232-1247

    • DOI

      10.1111/j.1529-8817.2012.01213.x

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Two new ASP toxin production types in strains of Nitzschia navis-varingica from the Philippines2012

    • Author(s)
      Marc Lawrence J. Romero, Yuichi Kotaki, Juan Relox, Jr., Nina Lundholm, Yoshinobu Takata, Masaaki Kodama, Yasuwo Fukuyo, and Yuichi Kotaki
    • Journal Title

      Coast. Mar. Sci

      Volume: 35(1) Pages: 67-69

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Screening of diatoms that produce ASP toxins in southernmost Asian waters2012

    • Author(s)
      Hikmah Thoha, Yuichi Kotaki, Lily Panggabean, Nina Lundholm, Hisao Ogawa, Lim Po Teen, Yoshinobu Takata, Masaaki Kodama, Yasuwo Fukuyo
    • Journal Title

      Coast. Mar. Sci

      Volume: 35(1) Pages: 34-38

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Morphological observation of two species of Pseudo-nitzschia (Bacillariophyceae)2012

    • Author(s)
      Hong-Chang Lim, Po-Teen Lim, Suriyanti Nyun-Pau Su, Yuichi Kotaki, and Chui-Pin Leaw
    • Journal Title

      Coast. Mar. Sci

      Volume: 35(1) Pages: 52-57

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Screening of diatoms that produce ASP toxins in Southernmost Asian waters2012

    • Author(s)
      Hikmah Thoha, Yuichi Kotaki*, Lily PanggabeanNina Lundholm, Hisao Ogawa ,Po Teen Lim, Yoshinobu Takata, Masaaki Kodama and Yasuwo Fukuyo
    • Journal Title

      Coastal Marine Science

      Volume: 35 Pages: 34-38

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Two new ASP toxin production types in strains of Nitzschia navis-varingica from the Philippines2012

    • Author(s)
      Marc Lawrence J. ROMERO, Yuichi KOTAKI*, Juan RELOX, Jr ., Nina LUNDHOLM, Yoshinobu TAKATA, Masaaki KODAMA and Yasuwo FUKUYO
    • Journal Title

      Coastal Marine Science

      Volume: 35 Pages: 67-69

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Toxin production and temperature-induced morphological variation of the diatom Pseudo-nitzschia seriata from the Arctic2011

    • Author(s)
      Lene Rusz Hansen, Stine i Soylu, Yuichi Kotaki, Øjvind Moestrup, Nina Lundholm
    • Journal Title

      Harmful Algae

      Volume: 10 (6) Issue: 6 Pages: 689-696

    • DOI

      10.1016/j.hal.2011.05.004

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transcellular transport of domoic acid across intestinal Caco-2 cell monolayers2011

    • Author(s)
      Osamu Kimura, Yuichi Kotaki, Naoya Hamaue, Koichi Haraguchi, Tetsuya Endo
    • Journal Title

      Food and Chem Toxicol

      Volume: 49 Issue: 9 Pages: 2167-2171

    • DOI

      10.1016/j.fct.2011.06.001

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Unique amnesic shellfish toxin composition found in the South East Asian diatom Nitzschia navis-varingica2011

    • Author(s)
      Romero, M.L.J.
    • Journal Title

      Harmful Algae

      Volume: VOL.10 Issue: 5 Pages: 456-462

    • DOI

      10.1016/j.hal.2011.02.006

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] わが国の記憶喪失性貝毒生産珪藻Nitzschia navis-varingicaの分布特性2013

    • Author(s)
      小瀧裕一・吉田健一・境野智・品田祥汰・一番ヶ瀬裕司・松井麟太郎
    • Organizer
      平成25年度日本水産学会秋期大会
    • Place of Presentation
      三重大学
    • Year and Date
      2013-09-21
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 震災後の記憶喪失性貝毒生産底生珪藻Nitzschia navis-varingicaの分布調査2013

    • Author(s)
      小瀧裕一・一番ヶ瀬裕司・松井麟太郎・吉田健一・Lundholm Nina
    • Organizer
      平成25年度日本水産学会春期大会
    • Place of Presentation
      東京海洋大学品川キャンパス
    • Year and Date
      2013-03-29
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 記憶喪失性貝毒生産底生珪藻Nitzschia navis-varingicaの分布および毒生産特性-62012

    • Author(s)
      小瀧裕一・木田龍介・影山一成・原口浩一・Lundholm Nina
    • Organizer
      平成24年度日本水産学会秋期大会
    • Place of Presentation
      水産大学校下関市
    • Year and Date
      2012-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Factors affecting the ASP toxin production of a benthic diatom Nitzschia navis-varingica2012

    • Author(s)
      Yuichi Kotaki, Marc Lawrence J. Romero, Nina Lundholm, Juan R. Relox, Atsushi Kobiyama, Koichi Haraguchi
    • Organizer
      15^<th> International Conference on Harmful Algae, CECO
    • Place of Presentation
      Gyeongnam, Korea
    • Related Report
      2013 Final Research Report
  • [Presentation] Factors affecting the ASP toxin production of a benthic diatom Nitzschia navis-varingica2012

    • Author(s)
      Yuichi Kotaki, Marc Lawrence J. Romero, Nina Lundholm, Juan R. Relox, Atsushi Kobiyama, Koichi Haraguchi
    • Organizer
      The 15th International Conference on Harmful Alagae
    • Place of Presentation
      CECE, Gyeongnam, Korea
    • Related Report
      2012 Research-status Report
  • [Presentation] 記憶喪失性貝毒生産底生珪藻Nitzschia navis-varingicaの分布および毒生産特性-62012

    • Author(s)
      小瀧裕一・木田龍介・影山一成・原口浩一・Lundholm Nina
    • Organizer
      平成24年度日本水産学会秋期大会
    • Place of Presentation
      水産大学校
    • Related Report
      2012 Research-status Report
  • [Presentation] 記憶喪失性貝毒生産底生珪藻Nitzschia navis-varingicaの分布と毒生産特性-52011

    • Author(s)
      小瀧裕一・冨山崇・澤口敏哉・Lundholm Nina
    • Organizer
      平成23年度日本水産学会秋季大会
    • Place of Presentation
      長崎大学文教キャンパス
    • Year and Date
      2011-10-01
    • Related Report
      2013 Final Research Report
  • [Presentation] Diatom factory producing amnesic shellfish poisoning (ASP) toxin domoic acid and its isomers, BIT's2011

    • Author(s)
      Yuichi Kotaki
    • Organizer
      1^<st> Annual Congress of Marine Biotechnology-2011
    • Place of Presentation
      Dalian, China
    • Year and Date
      2011-04-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 記憶喪失性貝毒生産珪藻Nitzschia navis-varingicaの分布および毒生産特性ー52011

    • Author(s)
      小瀧裕一・冨山崇・澤口敏弥・Lundholm Nina
    • Organizer
      日本水産学会平成23年度秋季大会
    • Place of Presentation
      長崎大学文教キャンパス
    • Related Report
      2011 Research-status Report
  • [Presentation] 震災後の記憶喪失性貝毒生産珪藻出現調査

    • Author(s)
      小瀧裕一・吉田健一・ 境野智・品田祥太
    • Organizer
      岩手県水産技術センター公開合同セミナー(第3回)「震災後3年、海はどう変わったのか?~よみがえる環境と生物~」
    • Place of Presentation
      岩手県大船渡地区合同庁舎
    • Related Report
      2013 Annual Research Report
  • [Book] Ecology and oceanography of harmful merine microalgae (projecto-2). In Coastal Marine Science in Southeast Asia (Synthesis Report of the Core University Program of the Japan Society for the Promotion of Science: Caoastal Marine Science (2001-2010)2011

    • Author(s)
      Yasuwo Fukuyo, Masaaki Kodama, Takuo Omura, Ken Furuya, Elsa F. Furio, Mirriam Cayme, Lim Po Teen, Dao Viet Ha, Yuichi Kotaki, Kazumi Matsuoka, Mitsunori Iwataki et al.
    • Total Pages
      26
    • Publisher
      Terrapub, Tokyo
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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