| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
An isolation method of microsatellite DNA markers was developed for the evaluation of genetic diversity of organisms inhabiting rural areas. This method was composed of five processes such as DNA extraction, DNA fragmentation, screening of microsatellite regions, etc. Applying this method, nineteen to forty of novel microsatellite DNA markers were isolated and characterized for seven species of fish, dragonfly and mysid collected from agricultural canals. Furthermore, these markers were available for practical analysis of their genetic diversity and the structures of their populations. The development of the markers for other species based on this method and the investigation of genetic diversity using the developed markers are expected in the near future.
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