Analysis of epigenetic characters in bovine IVF embryos.

• Project/Area Number: 23580384
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Iwate University
• Principal Investigator: SAWAI Ken 岩手大学, 農学部, 准教授 (90390864)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: IVF胚 / 遺伝子発現 / 体外発生 / ウシ / アポトーシス / 発生工学 / 初期胚 / 体外受精

Research Abstract

The objectives of this study were to determine the effects of protein components, growth factors and progesterone (P4) on development competence and expression of apoptosis-related genes of bovine IVF embryos. Our results suggest differences in levels of transcripts of apoptosis related genes in bovine embryos cultured with or without GF. Protein components, growth factors and P4 in culture medium improve the development competence and change the gene transcript of bovine IVF embryos.

Research Products

• [Presentation] 体外発生培地へのプロジェステロン添加がウシ体外受精胚の発生におよぼす影響 (2013)
  • Author(s): 櫻井伸行,羽田雅紀,橋爪力,澤井健
  • Organizer: 第106回日本繁殖生物学会
  • Place of Presentation: 東京農工大(東京都)
  • Year and Date: 2013-09-13
• [Presentation] Epigenetic abnormality in bovine cloned embryos, and approaches for the overcoming (2013)
  • Author(s): Sawai, K.
  • Organizer: 2013 Biomodulation Symposium, Joining of Korea and Japan for the study on"From Germplasm to Cloning"
  • Place of Presentation: Seoul, Republic of Korea
  • Year and Date: 2013-06-05
• [Presentation] ウシ体細胞クローン胚のエピジェネティクス特性とその人為的制御 (2013)
  • Author(s): 澤井健
  • Organizer: NIAS/NILGS合同シンポジウム「動物生殖技術研究の現状と今後の展望」
  • Place of Presentation: エポカルつくば(茨城県)
  • Year and Date: 2013-02-26
• [Presentation] 体外発生培地へのプロジェステロン添加がウシ体外受精胚の発生におよぼす影響 (2013)
  • Author(s): 櫻井伸行, 羽田雅紀, 橋爪 力, 澤井 健
  • Organizer: 第106回日本繁殖生物学会
  • Place of Presentation: 東京農工大学
• [Presentation] Epigenetic abnormality in bovine cloned embryos, and approaches for the overcoming. (2013)
  • Author(s): Sawai, K
  • Organizer: 2013 Biomodulation Symposium, Joining of Korea and Japan for the study on "From Germplasm to Cloning"
  • Place of Presentation: Seoul National University, Seoul, Korea
  • Invited
• [Presentation] 体外発生培地への成長因子添加はウシ体外受精胚のアポトーシスを抑制する (2012)
  • Author(s): 舘山奈江,櫻井伸行,熊谷仁,寺田幸弘,澤井健
  • Organizer: 第15回日本IVF学会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2012-09-29
• [Presentation] Effects of protein components and growth factors on development competence and gene expression of bovine IVF embryos (2011)
  • Author(s): Tateyama, N., Hashizume, T. and Sawai, K.
  • Organizer: 2^<nd> World Congress on Reproductive Biology
  • Place of Presentation: Cairns, Australia
• [Presentation] Effects of protein components and growth factors on development competence and gene expression of bovine IVF embryos. (2011)
  • Author(s): Tateyama, N., Hashizume, T. and Sawai, K.
  • Organizer: 2nd World Congress on Reproductive Biology
  • Place of Presentation: Cairns convention centre (オーストラリア)
• [Presentation] 体外発生培地への成長因子添加はウシ体外受精胚のアポトーシスを抑制する.
  • Author(s): 舘山奈江, 櫻井伸行, 熊谷仁, 寺田幸弘, 澤井 健
  • Organizer: 第15回日本IVF学会
  • Place of Presentation: 大阪国際会議場（大阪府）
• [Presentation] ウシ体細胞クローン胚のエピジェネティクス特性とその人為的制御
  • Author(s): 澤井 健
  • Organizer: NIAS/NILGS合同シンポジウム「動物生殖技術研究の現状と今後の展望」
  • Place of Presentation: エポカルつくば（茨城県）
  • Invited