| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
The analysis of the conservation state of existing mammoth soft tissue (skin, muscle and bone marrow) by PCR and the development of the method to collect somatic cell nuclei from bone marrow were examined. In results, we succeeded to develop the efficient method to collect somatic cell nuclei from bone marrow. Also we succeeded to have new, well conserved mammoth's skin, muscle, hypodermis and bone marrow from Sakha (Yakutia) Republic. Furthermore, to improve developmental ability of reconstructed embryos, we tried to co-inject mitochondria which derived from cells of same species with donor cell nucleus. However the developmental ability of reconstructed embryos was not improved.
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