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In vitro growth of porcine growing oocytes

Research Project

Project/Area Number 23580397
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied animal science
Research InstitutionKinki University

Principal Investigator

HASHIMOTO Shu  近畿大学, 生物理工学部, 研究員 (30570949)

Co-Investigator(Kenkyū-buntansha) HOSOI Yoshihiko  近畿大学, 生物理工学部, 教授 (70192739)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords発生工学 / 生殖工学 / 生殖医療 / 卵母細胞
Research Abstract

We have focused attention on the mitochondrial activity of in vitro grown (IVG) oocytes and shown that L-Carnitine, which acts as a carrier of fatty acids across the inner mitochondrial membrane for subsequent beta-oxidation, supported the viability of growing oocytes. Moreover, we have successfully obtained fertilized ova by ICSI which developed from growing oocytes in vitro.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (32 results)

All 2014 2013 2012 2011

All Journal Article (14 results) (of which Peer Reviewed: 13 results,  Open Access: 1 results) Presentation (13 results) (of which Invited: 2 results) Book (5 results)

  • [Journal Article] Possible selection of viable human blastocysts after vitrification by monitoring morphological changes2014

    • Author(s)
      Maezawa T, Yamanaka M, Hashimoto S, Amo A, Ohgaki A, Nakaoka Y, Fukuda A, Ikeda T, Inoue M, Morimoto Y.
    • Journal Title

      J Assist Reprod Genet

      Volume: (in press) Issue: 8 Pages: 1099-1104

    • DOI

      10.1007/s10815-014-0260-x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cryopreservation in ART and concerns with contamination during cryobanking2014

    • Author(s)
      Larman MG, Hashimoto S, Morimoto Y, Gardner DK.
    • Journal Title

      Reprod Med Biol

      Volume: (in press) Issue: 3 Pages: 107-117

    • DOI

      10.1007/s12522-014-0176-2

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oral melatonin supplementation improves oocyte and embryo quality in women undergoing in vitro fertilization-embryo transfer2014

    • Author(s)
      Nishihara T, Hashimoto S, Ito K, Nakaoka Y, Matsumoto K, Hosoi Y, Morimoto Y.
    • Journal Title

      Gynecological Endocrinology

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oral melatonin supplementation improves oocyte and embryo quality in women undergoing in vitro fertilization-embryo transfer2014

    • Author(s)
      Nishihara T, Hashimoto S, Ito K, Nakaoka Y, Matsumoto K, Hosoi Y, Morimoto Y
    • Journal Title

      Gynecological Endocrinology.

      Volume: n press Issue: 5 Pages: 359-362

    • DOI

      10.3109/09513590.2013.879856

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A closed system supports the developmental competence of human embryos after vitrification2013

    • Author(s)
      Hashimoto S, Amo A, Hama S, Ohsumi K, Nakaoka Y, Morimoto Y.
    • Journal Title

      J Assist Reprod Genet

      Volume: 30 Issue: 3 Pages: 371-376

    • DOI

      10.1007/s10815-012-9928-2

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Growth retardation in human blastocysts increases the incidence of abnormal spindles and decreases implantation potential after vitrification2013

    • Author(s)
      Hashimoto S, Amo A, Hama S, Ito K, Nakaoka Y, Morimoto Y.
    • Journal Title

      Hum Reprod

      Volume: 28 Issue: 6 Pages: 1528-1535

    • DOI

      10.1093/humrep/det059

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Good thermally conducting material supports follicle morphologies of porcine ovaries cryopreserved with ultrarapid vitrification2013

    • Author(s)
      Hashimoto S, Suzuki N, Amo A, Yamochi T, Hosoi Y, Morimoto Y.
    • Journal Title

      J Reprod Dev

      Volume: 59 Pages: 496-499

    • NAID

      130003361046

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hippo Signaling Disruption and Akt Stimulation of Ovarian Follicles for Infertility2013

    • Author(s)
      Kawamura K, Cheng Y, Suzuki N, Deguchi M, Sato Y, TakaeS, Ho CH, Kawamura N, Tamura M, Hashimoto S, Sugishita Y, Morimoto Y, Hosoi Y, Yoshioka N, Ishizuka B, Hsueh AJ.
    • Journal Title

      Proc Nat Acad Sci USA

      Volume: 110 Issue: 43 Pages: 17474-17479

    • DOI

      10.1073/pnas.1312830110

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cysteamine Supplementation During In Vitro Maturation (IVM) of Rabbit Oocyte Improves the Developmental Capacity after Intracytoplasmic Sperm Injection2013

    • Author(s)
      Miyamoto Y, Teramura T, Izumi H, Hashigami Y, Hashimoto S, Fukuda K, Morimoto Y, Hosoi Y.
    • Journal Title

      Reprod Med Biol

      Volume: 12 Issue: 4 Pages: 179-185

    • DOI

      10.1007/s12522-013-0157-x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selection of high potential embryos by culture in poly-(dimethylsiloxane) microwells and time-lapse imaging2012

    • Author(s)
      Hashimoto S, Kato N, Saeki K, Morimoto Y.
    • Journal Title

      Fertil Steril

      Volume: 97 Issue: 2 Pages: 332-337

    • DOI

      10.1016/j.fertnstert.2011.11.042

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Assessment of long term function of heterotopic transplants of vitrified ovarian tissue in cynomolgus monkeys.2012

    • Author(s)
      Suzuki N, Hashimoto S et al.
    • Journal Title

      Human Reproduction

      Volume: 27 Issue: 8 Pages: 2420-2429

    • DOI

      10.1093/humrep/des178

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] がん・生殖医療の実践に基づいた化学療法後の妊孕性温存の可能性について2012

    • Author(s)
      鈴木直、吉岡伸人、杉下陽堂、高江正道、洞下由記、五十嵐豪、橋本周、森本義晴、石塚文平
    • Journal Title

      癌と化学療法

      Volume: 39 Pages: 151-157

    • Related Report
      2013 Final Research Report
  • [Journal Article] 酸素消費量に基づいた凍結融解胚の選別2012

    • Author(s)
      橋本 周、山中昌哉、天羽杏実、森本義晴
    • Journal Title

      J Mamm Ova Res

      Volume: 29 Pages: 175-179

    • NAID

      10031117489

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Developmental assessment of human vitrified-warmed blastocycts based on oxygen consumption2011

    • Author(s)
      Masaya Yamanaka
    • Journal Title

      Hum. Reprod.

      Volume: 26 Issue: 12 Pages: 3366-3371

    • DOI

      10.1093/humrep/der324

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒト発育遅延胚の発育経過のイメージング:紡錘体形成異常の増加と着床能の低下2013

    • Author(s)
      橋本周、山縣一夫、森本義晴
    • Organizer
      第31回日本受精着床学会総会・学術講演会
    • Place of Presentation
      別府国際コンベンションセンター(大分県別府市)
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト発育遅延胚では紡錘体形成異常が増加し、着床能が低下した2013

    • Author(s)
      橋本周、天羽杏実、濱聡子、伊藤啓二朗、中岡義晴、森本義晴
    • Organizer
      第31回日本受精着床学会総会・学術講演会
    • Place of Presentation
      別府国際コンベンションセンター(大分県別府市)
    • Related Report
      2013 Final Research Report
  • [Presentation] Does growth retardation in human blastocysts decrease implantation potential after embryo transfer through an increase of the abnormal spindles?2013

    • Author(s)
      Hashimoto S, Amo A, Ito K, Nakaoka Y, Morimoto Y
    • Organizer
      The 29th Annual Meeting of The European Society of Human Reproduction and Embryology
    • Place of Presentation
      ExCel London (London, UK)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒト発育遅延胚では紡錘体形成異常が増加し、着床能が低下した2013

    • Author(s)
      橋本 周、天羽杏実、濱 聡子、伊藤啓二朗、中岡義晴、森本義晴
    • Organizer
      第31回日本受精着床学会学術講演会
    • Place of Presentation
      別府B-Con Plaza (大分県別府市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒト発育遅延胚の発育経過のイメージング:紡錘体形成異常の増加と着床能の低下2013

    • Author(s)
      橋本 周、山縣一夫、森本義晴
    • Organizer
      第31回日本受精着床学会学術講演会
    • Place of Presentation
      別府B-Con Plaza (大分県別府市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Mitochondrial DNA Increases Even in the Final Stretch of Human Oocyte Growth2013

    • Author(s)
      Hashimoto S, Goto H, Amo A, Yamochi T, Mitani T, Inoue M, Morimoto Y
    • Organizer
      The 4th International Symposium on Dynamics of Mitochondria
    • Place of Presentation
      沖縄残波岬ロイヤルホテル (沖縄県中頭郡読谷村)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development estimation of human frozen-thawed blastocysts based on their oxygen consumption2012

    • Author(s)
      Hashimoto S.
    • Organizer
      The 4th Japan-Korea ART Conference 2012
    • Place of Presentation
      大阪大学中之島センター(大阪府大阪市)
    • Year and Date
      2012-03-24
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト胚個別培養系の構築と経時的な形態変化を取り入れた胚の発育能力の予測に関して2012

    • Author(s)
      橋本周、加藤暢宏、佐伯和弘、森本義晴
    • Organizer
      第30回日本受精着床学会総会・学術講演会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト初期胚発育過程における染色体挙動変化の可視化2012

    • Author(s)
      橋本周、中岡義晴、天羽杏実、中野達也、山縣一夫、森本義晴
    • Organizer
      第30回日本受精着床学会総会・学術講演会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Related Report
      2013 Final Research Report
  • [Presentation] Live cell imaging reveals human chromosome dynamics during embryo development2012

    • Author(s)
      Hashimoto S, Nakaoka Y, Amo A, Nakano T, Yamagata K, Morimoto Y.
    • Organizer
      The 28th Annual Meeting of ESHRE
    • Place of Presentation
      Istanbul Congress Center (トルコ、イスタンブール)
    • Related Report
      2013 Final Research Report
  • [Presentation] Live cell imaging reveals human chromosome dynamics during embryo development2012

    • Author(s)
      Hashimoto S, Nakaoka Y, Amo A, Nakano T, Yamagata K, Morimoto Y
    • Organizer
      The 28th Annual Meeting of The European Society of Human Reproduction and Embryology
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒト胚個別培養系の構築と経時的な形態変化を取り入れた胚の発育能力の予測に関して2012

    • Author(s)
      橋本 周、加藤暢宏、佐伯和弘、森本義晴
    • Organizer
      第30回日本受精着床学会学術講演会
    • Place of Presentation
      大阪国際会議場(大阪府)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Development estimation of human frozen-thawed...2012

    • Author(s)
      Shu Hashimoto
    • Organizer
      The 4th Japan-Korea ART Conference 2012(招待講演)
    • Place of Presentation
      大阪大学中之島センター(大阪府)
    • Related Report
      2011 Research-status Report
  • [Book] 卵巣組織の超急速凍結法, 卵巣組織凍結・移植新しい妊孕性温存療法の実践2013

    • Author(s)
      橋本周、鈴木直、森本義晴
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2013 Final Research Report
  • [Book] 卵巣組織のガラス化保存法: Cryo Support (Cryo Kit Type M), 卵巣組織凍結・移植新しい妊孕性温存療法の実践2013

    • Author(s)
      杉下陽堂、橋本周、星名真理子、川越雄太、吉岡伸人、高江正道、洞下由記、五十嵐豪、津田千春、矢持隆之、竹之下誠、細井美彦、河村和弘、森本義晴、鈴木直
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2013 Final Research Report
  • [Book] 卵巣組織凍結, がん・生殖医療:妊孕性温存の診療2013

    • Author(s)
      橋本周、鈴木直、河村和弘、杉下陽堂、森本義晴
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2013 Final Research Report
  • [Book] がん・生殖医療:妊孕性温存の診療2013

    • Author(s)
      橋本 周、鈴木 直、河村和弘、杉下陽堂、森本義晴
    • Total Pages
      289
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2013 Annual Research Report
  • [Book] 卵巣組織凍結・移植 新しい妊孕性温存療法の実践2013

    • Author(s)
      橋本 周、鈴木 直、森本義晴
    • Total Pages
      153
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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