| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
The effects of metabolism of purine derivatives on bovine embryo development were investigated. When de novo purine synthesis was inhibited, the embryo development after maternal-to-zygotic transition (MZT) was severely compromised. Moreover, when the purine derivatives (adenine or hypoxanthine) were supplemented in the presence of the inhibitor for de novo purine synthesis, the inhibitory effect disappeared. These results suggested that purines are necessary for bovine embryo development after MZT. However, excess amount of purine derivatives (especially adenine) deteriorated bovine embryo development. In the presence of excess amount of adenine after fertilization, the cleavage of the embryo was arrested and the fragmentation of nuclear was induced.
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