| Project/Area Number |
23580400
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Gifu University |
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Principal Investigator |
NAGANO Isao 岐阜大学, 医学(系)研究科(研究院), 准教授 (40283296)
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| Co-Investigator(Kenkyū-buntansha) |
WU Zhiliang 岐阜大学, 医学系研究科, 助 教 (90313874)
KAMATARI Yuji 岐阜大学, 生命科学総合研究 支援センター, 助教 (70342772)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 旋毛虫 / 免疫抑制 / 分泌タンパク質 / 組換えタンパク質 / サイトカイン / マクロファージ / 高次構造解析 / X線結晶構造解析 / 立体構造解析 |
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| Research Abstract |
Our previous study indicated that Trichinella pseudospirlais infection can ameliorate autoimmune encephalomyelitis through suppressing Th17 and Th1 responses. In the present study, we investigated the immunomodulatory function of the recombinant T. pseudospiralis 53kDa excretory-secretory protein (Tp53), including the effect on the production of IL-6 of macrophage and IL-17, IFN-gamma and IL-4 of splenocyte in vitro and in vivo. Results suggested the recombinant Tp53 has immune regulatory function of inhibiting IL-6 production of macrophage, and suppressing Th17 and Th1 responses of splenocyte, suggesting Tp53 possesses immunomodulation function to suppress Th17 response via inhibiting IL-6 and IL-17 positive feedback loop.
For structural studies, we have established expression and purification system using E. coli and pCold-GST vector. We have started the crystallization of Tp53. However, we have not obtain any crystal yet.
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