| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Neutrophil accumulation is a critical event in the pathogenesis of lung inflammation. Myeloperoxidase (MPO) and phagocyte NADPH oxidase, major constituents of neutrophils that generate reactive oxygen species (ROS), contribute to microbial killing. This study examined the role of these enzymes in neutrophil recruitment into the zymozan- or nonviable Candida albicans (nCA)-exposed lung of mice. Mice deficient in MPO and phagocyte NADPH oxidase that pulmonary received zymosan and nCA showed more severe pneumonia than wild-type mice. This was associated with higher levels of several inflammatory mediators in the lungs of these mutant mice. Enhanced production of chemokine by the mutant neutrophils, concomitant with up-regulation of Syk/ERK/NF-kB pathway, may at least partly contribute to exacerbated inflammation in the mutant mice. Thus, loss of ROS production by neutrophils causes significant abnormalities in both host defense and inflammation.
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