| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
Histone Deacetylases (HDACs) are known to play important roles during preimplantation development. However, the roles of acetylation of non-histone proteins mouse embryos still remains poorly understood. In this study, we have focused on acetylation of alpha-tubulin and HDACs in terms of oocyte quality during one-cell stage. First, we found that treatment with NAM, nicotinamide, an inhibitor for Class III HDACs inhibited cellular fragmentation and spindle elongation after postovulatory in vitro aging. Also, the alpha-tubulin increased acetylation during aging, suggesting not only histone but non-histone protein acetylation also increases with oocyte aging. Further, once an oocyte has been activated, histone and nonhistone proteins are hyperacetylated partly due to a reduction of HDAC activity. TSA treatment of zygotes enhances their acetylation. Thus, we provide the evidence that protein acetylation play important roles for oocyte quality which affect subsequent embryonic development.
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